Intraspecific Relationship of Plasmopara halstedii Isolates Differing in Pathogenicity and Geographic Origin Based on ITS Sequence Data

Spring, Otmar; Bachofer, Mark; Thines, Marco; Riethmüller, Alexandra; Göker, Markus; Oberwinkler, Franz

doi:10.1007/s10658-005-5996-9

Cited by 49 publications

(25 citation statements)

References 26 publications

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“…Either the isolates used in our study were different from the ones used by Delmotte et al (2008), or the intrarace variance in the EST-derived marker may explain the different results reported. However, the distinctiveness of the 7xx races compared to those of races 100 or 3xx has recently been shown based on ITS sequence data (Spring et al, 2006). No correlation was detected between aggressiveness traits (Table 2) and EST genotypes.…”

Section: Discussionmentioning

confidence: 93%

“…Today, the total P. halstedii races can overcome the resistance genes in seven of the nine downy mildew differential lines of H. annuus, and the Pl2 and Pl6 genes have each been overcome by eight races . Moreover, Spring et al (2006) differentiated between populations of pathotypes 100, 310 and 330 and a group of populations representing pathotypes 700, 701, 703, 710 and 730. Also, Delmotte et al (2008) identified three genetically differentiated groups of isolates organized around the first three races described in France: 100, 710 and 703.…”

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confidence: 99%

“…Concerning this pathosystem, several studies have been carried on virulence (Delmotte et al, 2008;Gulya, 2007;Spring et al, 2006;Tourvieille de Labrouhe et al, 2000, 2010 and more recently on aggressiveness (Sakr, 2009;2010). Virulence has been defined as specific, diseasecausing abilities and aggressiveness as non-specific, diseasecausing abilities (Van der Plank, 1968).…”

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confidence: 99%

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Aggressiveness in Plasmopara halstedii (sunflower downy mildew)

Sakr¹

2011

The Plant Pathology Journal

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Aggressiveness was studied in seven Plasmopara halstedii (sunflower downy mildew) pathotypes: 100, 300, 304, 314, 704, 710 and 714. Aggressiveness criteria including percentage infection, latent period, sporulation density and reduction of hypocotyl length (dwarfing) were analysed in one sunflower inbred line showing a high level of quantitative resistance. Genetic relationships were detected between the seven pathotypes using 12 ESTderived markers. Pathotypes 100, 300, 304 and 314 were characterized with shorter latent period and higher sporulation density than pathotypes 710, 704 and 714. All pathotypes showed high percentage infection values and caused a large reduction in seedling size except for pathotype 314 involved in dwarfing. Pathotypes 714, 704 and 314 had an intermediary genetic position between the pathotypes 100 and 710. No correlation was detected between aggressiveness traits and EST genotypes.

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Section: Discussionmentioning

confidence: 93%

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confidence: 99%

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confidence: 99%

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Aggressiveness in Plasmopara halstedii (sunflower downy mildew)

Sakr¹

2011

The Plant Pathology Journal

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“…However, Table 2 Fatty acid composition (ratio of total peak area) assayed by GC analysis of sporangia and sporangiophores of P. angustiterminalis propagated on sunflower lines and (Thines 2007). The total ITS length (3190 bp) exceeded the known size found so far within the Plasmopara genus by >20% (Spring et al 2006;Voglmayr et al 2006). For the phylogenetic analysis only conserved regions were used in the model to exclude the influences of in/dels (Mitchell and Zuccaro 2006).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of a Plasmopara angustiterminalis isolate from Xanthium strumarium

Komjáti

Walcz²,

Virányi

et al. 2007

Eur J Plant Pathol

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Leaves of Xanthium strumarium infected with downy mildew were collected in the vicinity of a sunflower field in southern Hungary in 2003. Based on phenotypic characteristics of sporangiophores, sporangia and oospores as well as host preference the pathogen was classified as Plasmopara angustiterminalis. Additional phenotypic characters were investigated such as the size of sporangia, the number of zoospores per sporangium and the time-course of their release. Infection studies revealed infectivity of the P. angustiterminalis isolate to both X. strumarium and Helianthus annuus. Inoculation of the sunflower inbred line, HA-335 with resistance to all known P. halstedii pathotypes, resulted in profuse sporulation on cotyledons and formation of oospores in the bases of hypocotyls. Infections of sunflower differential lines often led to damping-off. Molecular genetic analysis using simple sequence repeat primers and nuclear rDNA sequences revealed clear differences to Plasmopara halstedii, the downy mildew pathogen of sunflower.

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“…Whereas this primer has so far not found use in oomycete community studies, it has been used successfully to amplify the DNA of a wide range of oomycetes in phylogenetic research (Spring et al 2006, Thines 2007.…”

Section: Introductionmentioning

confidence: 99%

Oomycete-specific ITS primers for identification and metabarcoding

Riit¹,

Tedersoo²,

Drenkhan³

et al. 2016

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Microbial metabarcoding studies using high throughput sequencing technologies generate unprecedented amounts of DNA sequence data and make it possible to determine not only the composition of the communities but also the underlying factors powering the evolution of these communities. Despite the potential of community level studies in helping to better understand the ecology of pathogens and to manage the losses caused by them, very few oomycete addressing metabarcoding studies have been carried out and with highly variable results. The aim of this study was to develop new oomycete-specific ITS region PCR primers with improved specificity for metabarcoding and identification of oomycetes. The modified ITS1oo and the newly developed ITS3oo primers show improved in silico specificity for oomycetes and when paired with the universal ITS4 successfully amplified the DNA from all eleven tested oomycete species from six genera. High throughput sequencing of 20 soil samples from forest nurseries and bordering areas, using the primer pair ITS1oo/ITS4, recovered more than 400 oomycete OTUs, which is a significant increase over previous studies, and indicates the ability of the new method to detect various oomycete groups from complex substrates. The average fraction of oomycete reads per soil samples was 32-36%, with a maximum of 69%. The recovered oomycete OTUs represented the groups Lagenidiales, Peronosporales, Pythiales and Saprolegniales, with Pythiales dominating in all samples. In addition, the new primers were successfully used in identifying pathogens directly from infected plant tissues with Sanger sequencing. The pathogen was identified to the species or genus level in four samples out of six. In conclusion, the developed oomycete-specific primers provide a reliable method for the identification and metabarcoding of oomycetes.Copyright Taavi RESEARCH ARTICLETaavi Riit et al. / MycoKeys 14: 17-30 (2016) 18

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Intraspecific Relationship of Plasmopara halstedii Isolates Differing in Pathogenicity and Geographic Origin Based on ITS Sequence Data

Cited by 49 publications

References 26 publications

Aggressiveness in Plasmopara halstedii (sunflower downy mildew)

Aggressiveness in Plasmopara halstedii (sunflower downy mildew)

Characteristics of a Plasmopara angustiterminalis isolate from Xanthium strumarium

Oomycete-specific ITS primers for identification and metabarcoding

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