Intraspecifïc variation in gel electrophoresis patterns of soluble mycelial proteins of Phytophthora megasperma isolated from alfalfa

Abstract

The electrophoretic patterns of the buffer-soluble proteins of 26 isolates of Phytophthora megasperma f. sp. medicaginis from diseased alfalfa plants and soil were studied. On the basis of the protein patterns, the isolates were divided into two groups which correlated with cardinal temperatures for growth, pathogenicity, and oogonia size. Group 1 (seven isolates) has minimum growth at 5 °C, optimum at 25–30 °C, and maximum at 35 °C, and is a highly pathogenic pathotype with small oogonia. Group 2 (19 isolates) has minimum growth at less than 5 °C, optimum at 20 °C, and maximum at 30 °C, and is a less pathogenic pathotype with large oogonia. The electrophoresis technique was useful in identifying differences among the Phytophthora isolates.

Plant Research Centre, Agriculture Canada

“…It has been reported that LDS-gel electrophoresis (Faris et al 1986) showed nearly identical protein patterns within each of the two morphological groups, with much larger differences between them. Isolates of Gl and G2 also differ in cardinal temperatures for growth as shown herein, and in pathogenicity (Faris et al 1983, Faris 1984 it seems that there are two distinct species of Phylophthora causing PRR of alfalfa.…”

Comparative studies on the effect of benomyl on growth and ultrastructureof two isolates ofphytophthorainfestansfrom Egypt

“…One of the promising physiological methods for the identification and classification of Phytophthora spp. is the gel electrophoresis of their soluble proteins (Faris et al 1986). It has proved more convenient than serology, although the latter has other advantages (Gallegly 1983).…”

Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina

“…Such methods cannot be used to study the interaction between different isolates of M. phaseolina, or with ecological studies where a small quantity of inoculum has to be detected. Biochemical methods such as protein electrophoresis and fatty acid and isozyme profiles have also been applied for the characterization and differentiation of fungal taxa (Buth, 1984;Faris et al, 1986;Micales et al, 1992). However, the use of these techniques is limited to taxonomic purposes and to identification and detection of the fungi from pure culture.…”