Intraspecific Variation in Genome Size: A Critical Reassessment

Greilhuber, Johann

doi:10.1006/anbo.1998.0725

Cited by 201 publications

(163 citation statements)

References 62 publications

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“…Our data on nuclear DNA content in L. serriola, with such limited variation among 10 samples, tend to support the view of Greilhuber (1998).…”

Section: Dna Content Variation In L Serriolasupporting

confidence: 83%

“…Although some works confirm the existence of noticeable intraspecific variability in the content of nuclear DNA (Ohri, 1998), other authors consider nuclear DNA content as a species-specific character (Greilhuber, 1998). Our data on nuclear DNA content in L. serriola, with such limited variation among 10 samples, tend to support the view of Greilhuber (1998).…”

Section: Dna Content Variation In L Serriolasupporting

confidence: 63%

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North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

Lebeda¹,

Doležalová²,

Kitner³

et al. 2011

Acta Hortic.

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“…Our data on nuclear DNA content in L. serriola, with such limited variation among 10 samples, tend to support the view of Greilhuber (1998).…”

Section: Dna Content Variation In L Serriolasupporting

confidence: 83%

Section: Dna Content Variation In L Serriolasupporting

confidence: 63%

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

Lebeda¹,

Doležalová²,

Kitner³

et al. 2011

Acta Hortic.

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“…Genome size has been demonstrated to often vary a factor 1.5-3 between taxa with identical chromosome numbers (Ohri 1998;Yokoya et al 2000;Zonneveld 2001;Zonneveld and van Iren 2001;Ellul et al 2002;Zonneveld et al 2003a, b;Hirsch et al 2004: Albach andGreilhuber 2004;Zonneveld and Duncan 2006). Moreover, Greilhuber (1998Greilhuber ( , 2005 has clearly shown that intraspecific variation of genome size is much less than assumed. The evolution of genome size (Cx-value, Greilhuber 1979) has received increased attention during recent years (Bennett and Leitch 2005).…”

Section: Introductionmentioning

confidence: 99%

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

Zonneveld

2008

Plant Syst Evol

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The taxonomy of all species of Narcissus (Amaryllidaceae), an important horticultural crop, has not been investigated recently. As a new approach, genome size was determined by flow cytometry with propidium iodide from 375 accessions. The somatic nuclear DNA contents (2C) were shown to range from 14 to 38 pg for the diploids. Narcissus assoanus and N. gaditanus are, based on their nuclear DNA content, removed from section Apodanthi and placed in a new section Juncifolii. The different ploidy levels and species involved were entangled for N. ''fernandesii'' s.l. and a new allotetraploid form is named here. Section Pseudonarcissus was much more heterogeneous in nuclear DNA content than expected. Sixty-five accessions of N. pseudonarcissus possessed, with 23.7 pg, similar amounts of DNA. However, several species from this section were clearly distinctive in nuclear DNA content. It runs from the diploid N. primigenius with 21.7 pg to the also diploid N. nevadensis with 38.2 pg. Also N. abscissus and N. moleroi are with about 26 pg clearly different from N. pseudonarcissus. For the first time, in 11 accessions, hexaploidy was found in N. pseudonarcissus ssp. bicolor. A new section Nevadensis with 30-39 pg of nuclear DNA was split off from the section Pseudonarcissus with now 21-27 pg. A nonoploid N. dubius with 96.3 pg has by far the highest amount of nuclear DNA and can be calculated to have the highest ploidy ever reported in Narcisssus. The total number of Narcissus species was determined as 36, nine more than in Flora Europaea and they were divided up in two subgenera and 11 sections. Flow cytometry is shown to produce easily obtainable and original systematic data that lead to new insights. Genome size or C-value turns out to be one of the most salient features to define the status of the species in the genus Narcissus.

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“…As the accurate amount of nuclear DNA content is extremely important to understand the hereditary constituent of an organism, flow cytometry, originally developed for medical studies, is an easy, rapid, accurate and convenient tool for estimating plant genome size, ploidy level, assessing DNA content and analyzing the cell cycle (Greilhuber, 1998;Winkelmann et al, 1998). Datta and Naug (1968) Voluminous information on the nuclear DNA contents in plants is being published by Bennett et al (2000) and Hanson et al (2001).…”

Section: Introductionmentioning

confidence: 99%

FLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA BETWEEN OKRA LANDRACES (<i>ABELMOSCHUS ESCULENTUS</i> L.)

Salameh¹

2014

American Journal of Agricultural and Biological Sciences

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Okra Abelmoschus esculentus L. (Moench), is an economically important vegetable crop grown in tropical and sub-tropical as well as Mediterranean countries. The genome size of the Abelmoschus genus species is still rare. Ploidy determinations have traditionally been done by counting chromosomes of stained root tips, but this method is laborious and often difficult with species which have small chromosomes and high ploidy levels and can lead to misclassified. Flow cytometry has been widely used in various aspects of plant research, such as the determination of the DNA nuclear content. Nuclear DNA size of fifteen okra genotype was measured using flow cytometry. The data showed variation between Turkish genotype and Jordanian genotype. Genome size of okra ranged from 3.98 pg 2C −1 in Jordanian landrace to 17.67pg 2C −1 in Turkish landrace. The 2C genome size in Mbp ranged from 3897-17321 among Abelmoschus esculentus genotypes. Further studies is recommended to accurate chromosome count to be linked with the genome size.

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Intraspecific Variation in Genome Size: A Critical Reassessment

Cited by 201 publications

References 62 publications

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

FLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA BETWEEN OKRA LANDRACES (<i>ABELMOSCHUS ESCULENTUS</i> L.)

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