Intraspecific variation in thermotolerance and morphology of the invasive European green crab, Carcinus maenas, on the west coast of North America

Kelley, Amanda L.; Rivera, Catherine E. de; Buckley, Bradley A.

doi:10.1016/j.jembe.2011.08.005

Cited by 46 publications

(45 citation statements)

References 66 publications

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“…S3). However, it contrasts with a value of 34.7°C found by a previous study for 6°C-acclimated animals collected 3 years previously from the same site (Kelley et al, 2011). While the methods of these two studies differ, this discrepancy suggests that the green crab population may not be stable over time at this specific site, an artificial lagoon with a recent history of heavy C. maenas removal, surrounded by suburban housing and potentially isolated from the surrounding environment (Ritter, 1970) (C.K.T., unpublished observation).…”

Section: Discussion Eurythermalitycontrasting

confidence: 99%

Master of all trades: thermal acclimation and adaptation of cardiac function in a broadly distributed marine invasive species, the European green crab,Carcinus maenas

Tepolt

Somero

2014

Journal of Experimental Biology

128

126

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As global warming accelerates, there is increasing concern about how ecosystems may change as a result of species loss and replacement. Here, we examined the thermal physiology of the European green crab (Carcinus maenas Linnaeus 1758), a globally invasive species, along three parallel thermal gradients in its native and invasive ranges. At each site, we assessed cardiac physiology to determine heat and cold tolerance and acclimatory plasticity. We found that, overall, the species is highly tolerant of both heat and cold, and that it survives higher temperatures than co-occurring native marine crustaceans. Further, we found that both heat and cold tolerance are plastic in response to short-term acclimation (18-31 days at either 5 or 25°C). Comparing patterns within ranges, we found latitudinal gradients in thermal tolerance in the native European range and in the invasive range in eastern North America. This pattern is strongest in the native range, and likely evolved there. Because of a complicated invasion history, the latitudinal pattern in the eastern North American invasive range may be due either to rapid adaptation post-invasion or to adaptive differences between the ancestral populations that founded the invasion. Overall, the broad thermal tolerance ranges of green crabs, which may facilitate invasion of novel habitats, derive from high inherent eurythermality and acclimatory plasticity and potentially adaptive differentiation among populations. The highly flexible physiology that results from these capacities may represent the hallmark of a successful invasive species, and may provide a model for success in a changing world. KEY WORDS: Acclimatory plasticity, Cardiac physiology, Local adaptation, Species invasion, Thermal tolerance INTRODUCTIONEnvironmental temperature exerts a pervasive influence on the physiology of ectothermic animals, from the level of molecular processes to that of broad-scale biogeographic patterning (Hochachka and Somero, 2002). As global temperatures rise, there is increasing concern about the ability of ectothermic animals to survive in their warming environments, and considerable effort is being expended to identify the physiological traits that may be of greatest importance in adapting to rising temperatures and to learn how these traits differ among species (Somero, 2010).One aspect of success in a changed thermal environment comprises a combination of broad intrinsic tolerance of acute changes in temperature and a high capacity for acclimatization (phenotypic 'plasticity') during extended exposure to a new thermal regime (Simons, 2011;Healy and Schulte, 2012). These two abilities may largely govern how eurythermal an ectothermic species is, and thus help determine its chances for success in a warming world, particularly over short time scales. RESEARCH ARTICLEStanfordBecause maintenance of broad thermal tolerance may be metabolically costly and, indeed, unnecessary for animals inhabiting narrow thermal niches, ectothermic species range from extreme eurytherms to na...

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Section: Discussion Eurythermalitycontrasting

confidence: 99%

Master of all trades: thermal acclimation and adaptation of cardiac function in a broadly distributed marine invasive species, the European green crab,Carcinus maenas

Tepolt

Somero

2014

Journal of Experimental Biology

128

126

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“…Nonetheless, the lack of difference in CTMax values suggests limited acclimatory plasticity (in accordance with Stillman and Somero 2000;Tomanek 2010) related to different habitats and no local adaptation in upper tolerance limits. There is mixed evidence in relation to intraspecific differences in ectothermic organisms coming from different environments (e.g., MacIsaac et al 1985;Smale and Rabeni 1995;Lohr et al 1996;Strange et al 2002;Winne and Keck 2005;Fangue et al 2006;Kelley et al 2011). It is possible that intraspecific differences due to latitude or habitat depend on how steep the thermal gradient is, leading or not to local adaptation of thermal tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Studies in several populations and species have shown that there is a great variation in HSP expression patterns, mainly in three types of categories: as a function of thermal history, correlating to microhabitat, and between species . These types of studies have been performed in several marine organisms such as fish (e.g., Dietz and Somero 1992;Norris and Hightower 2002;Buckley and Hofmann 2002;Fangue et al 2006), bivalves (e.g., Chapple et al 1998;Buckley et al 2001;Helmuth and Hofmann 2001;Encomio and Chu 2005), snails (e.g., Somero 1999, 2002;Tomanek and Sanford 2003;Clark and Peck 2009), and crustaceans (e.g., Botton et al 2006;Kelley et al 2011). Since most of the studies on intraspecific differences in thermal tolerance focus on latitudinal, intertidal distribution, and seasonal comparisons (see Hofmann 1999), it is important to approach intraspecific differences that have been less explored in marine organisms, especially sex and size differences.…”

Section: Introductionmentioning

confidence: 99%

Thermal tolerance of the crab Pachygrapsus marmoratus: intraspecific differences at a physiological (CTMax) and molecular level (Hsp70)

Madeira

Narciso

Cabral

et al. 2012

Cell Stress and Chaperones

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Temperature is one of the most important variables influencing organisms, especially in the intertidal zone. This work aimed to test physiological and molecular intraspecific differences in thermal tolerance of the crab Pachygrapsus marmoratus (Fabricius, 1787). The comparisons made focused on sex, size, and habitat (estuary and coast) differences. The physiological parameter was upper thermal limit, tested via the critical thermal maximum (CTMax) and the molecular parameter was total heat shock protein 70 (Hsp70 and Hsp70 plus Hsc70) production, quantified via an enzyme-linked imunosorbent assay. Results showed that CTMax values and Hsp70 production are higher in females probably due to different microhabitat use and potentially due to different hormonal regulation in males and females. Among females, non-reproducing ones showed a higher CTMax value, but no differences were found in Hsp70, even though reproducing females showed higher variability in Hsp70 amounts. As reproduction takes up a lot of energy, its allocation for other activities, including stress responses, is lower. Juveniles also showed higher CTMax and Hsp70 expression because they occur in greater shore heights and ageing leads to alterations in protein synthesis. Comparing estuarine and coastal crabs, no differences were found in CTMax but coastal crabs produce more Hsp70 than estuarine crabs because they occur in drier and hotter areas than estuarine ones, which occur in moister environments. This work shows the importance of addressing intraspecific differences in the stress response at different organizational levels. This study shows that these differences are key factors in stress research, climate research, and environmental monitoring.

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“…Morphospecies status of individuals with different morphology is occasionally become difficult to be defined (Heinrichs et al, 2004). However, large amount of studies proved that morphological variations are observed within species in a wide range of animal phyla (Kelley et al, 2011;Hepp et al, 2012). According to Tzeng et al (2000), morphological variations among geographically separated populations involve their genetic components which are also variable.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains

Nuryanto

Pulungsari

2017

J Bio Bio Edu

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Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was checked for giant gourami sequences available in GenBank. Kimura 2-Parameter genetic distances were calculated in MEGA6 software. Haplotype and nucleotide diversity within population and Φ st -value among populations were estimated in Arlequin software. Phylogenetic relationship was reconstructed using the neighbor-joining method in MEGA6 software based on Kimura 2-parameter model with 1000 pseudobootstraps. Taxonomic identification results in 99% sequences homology to Osphronemus goramy sequences (accession number KU984978.1 and AY763768.1), means that all strains belong to single species. Low genetic distances, medium haplotype and low-level nucleotide diversity were observed among strains. Pairwise Φ st -comparison indicates no genetic differences among Sumatera strain, whereas strong genetic structures observed between Sumatera and Mandiangin strains. The phylogenetic tree showed that Mandiangin formed separate subclades from other strains with bootstraps value of 100%. This finding has important implication for breeding sciences and efforts.

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Intraspecific variation in thermotolerance and morphology of the invasive European green crab, Carcinus maenas, on the west coast of North America

Cited by 46 publications

References 66 publications

Master of all trades: thermal acclimation and adaptation of cardiac function in a broadly distributed marine invasive species, the European green crab,Carcinus maenas

Master of all trades: thermal acclimation and adaptation of cardiac function in a broadly distributed marine invasive species, the European green crab,Carcinus maenas

Thermal tolerance of the crab Pachygrapsus marmoratus: intraspecific differences at a physiological (CTMax) and molecular level (Hsp70)

Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains

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