2018
DOI: 10.1016/j.jare.2017.10.007
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Intraspecific variations in Cyt b and D-loop sequences of Testudine species, Lissemys punctata from south Karnataka

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Cited by 14 publications
(7 citation statements)
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“…Thus, for processed food, the approach based on a single locus could require a second step with a complementary marker for the identification in the case of sister species or complex evolutionary patterns. In our study, the new primer pair is designed on D-loop, a regulatory non coding region with high potential power for species identification (Karaiskou et al, 2004;Lalitha & Chandavar, 2018). Such region has proven to be helpful for discriminating, to species level, the complex of S. colias-S. japonicus-S. australasicus, as already found for Thunnus genus and S. japonicus haplotypes (Mariani et al, 2015;Viñas & Tudela, 2009;Yan et al, 2015).The two-step barcoding approach, already suggested for other taxa (Jin et al, 2018;Pawlowski et al, 2012), consists in a first step, in which universal primers are used to reach at least the genus level, followed by specific primers targeting problematic taxa.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, for processed food, the approach based on a single locus could require a second step with a complementary marker for the identification in the case of sister species or complex evolutionary patterns. In our study, the new primer pair is designed on D-loop, a regulatory non coding region with high potential power for species identification (Karaiskou et al, 2004;Lalitha & Chandavar, 2018). Such region has proven to be helpful for discriminating, to species level, the complex of S. colias-S. japonicus-S. australasicus, as already found for Thunnus genus and S. japonicus haplotypes (Mariani et al, 2015;Viñas & Tudela, 2009;Yan et al, 2015).The two-step barcoding approach, already suggested for other taxa (Jin et al, 2018;Pawlowski et al, 2012), consists in a first step, in which universal primers are used to reach at least the genus level, followed by specific primers targeting problematic taxa.…”
Section: Discussionmentioning
confidence: 99%
“…Although the CR is usually thought to be the fastest-evolving region of the mitogenome ( Brown, George & Wilson, 1979 ), few reports explored the usefulness of the CR in phylogenetic analyses of lizards ( Lalitha & Chandavar, 2018 ). Our study evidenced that this region may be highly informative for a variety of studies of the genetic variability of mitogenomes in lizards.…”
Section: Discussionmentioning
confidence: 99%
“…Additionally, here we have to note that only mitochondrial markers were used in this study. These are widely used in phylogenetic studies [37,[44][45][46][47], although they do not provide any information if hybridisation has taken place between certain lineages. Whereas hybridisation is highly plausible in the study area, since several sampled populations show mixed-lineage origin (see Fig.…”
Section: Colour Figure Onlinementioning
confidence: 99%