Intraspinal transplantation of subventricular zone-derived neural progenitor cells improves phrenic motor output after high cervical spinal cord injury

Sandhu, Milap S; Ross, Heather H.; Lee, Kun‐Ze; Ormerod, Brandi K.; Reier, Paul J.; Fuller, David D.

doi:10.1016/j.expneurol.2016.06.007

Cited by 13 publications

(8 citation statements)

References 58 publications

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“…(2007) and Vroemen et al. (2003), among others, have already reported (Akesson et al., 2007, Boido et al., 2011, Cummings et al., 2006, Jin et al., 2016, Karimi-Abdolrezaee et al., 2006, Macias et al., 2006, Nishimura et al., 2013, Okada et al., 2005, Pallini et al., 2005, Parr et al., 2007, Pfeifer et al., 2004, Pfeifer et al., 2006, Piltti et al., 2013, Salazar et al., 2010, Sandhu et al., 2017, Vroemen et al., 2003). The littermate approach implemented might partially explain the long-term survival and high level of integration of NPCs.…”

Section: Discussionmentioning

confidence: 75%

“…NPCs can self-renew and differentiate into neurons and glial cells (Morshead et al., 1994) as well as form neurospheres in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) (Morshead et al., 1994, Reynolds and Weiss, 1992). NPCs transplanted into SCI survive (Akesson et al., 2007, Boido et al., 2011, Cummings et al., 2006, Jin et al., 2016, Karimi-Abdolrezaee et al., 2006, Macias et al., 2006, Nishimura et al., 2013, Okada et al., 2005, Pallini et al., 2005, Parr et al., 2007, Pfeifer et al., 2004, Pfeifer et al., 2006, Piltti et al., 2013, Salazar et al., 2010, Sandhu et al., 2017, Vroemen et al., 2003), migrate (Karimi-Abdolrezaee et al., 2006, Mligiliche et al., 2005, Nishimura et al., 2013, Pallini et al., 2005, Salazar et al., 2010), integrate (Boido et al., 2011, Salazar et al., 2010, Vroemen et al., 2003), and form functional synapses with neurons and act as neuronal relays (Ben-Hur, 2010, Bonner et al., 2011, Cummings et al., 2005, Hong et al., 2014, Karimi-Abdolrezaee et al., 2006, Lu et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Conflicting results concerning the differentiation potential of transplanted NPCs have been reported. While differentiation into all neural lineages has been observed (Boido et al., 2011, Brock et al., 2017, Hong et al., 2014), some authors could only detect differentiation into astrocytes (Macias et al., 2006, Mligiliche et al., 2005, Pallini et al., 2005, Ricci-Vitiani et al., 2006, Sandhu et al., 2017). Specific differentiation into glia but not neurons has also been described (Karimi-Abdolrezaee et al., 2006, Parr et al., 2007, Pfeifer et al., 2004, Pfeifer et al., 2006, Piltti et al., 2013, Salewski et al., 2015, Vroemen et al., 2003).…”

Section: Introductionmentioning

confidence: 99%

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Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery

et al. 2019

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Summary Long-term survival and integration of neural progenitor cells (NPCs) transplanted following spinal cord injury (SCI) have been observed. However, questions concerning the differentiation choice, the mechanism of action, and the contribution of NPCs to functional recovery remains unanswered. Therefore, we investigated the differentiation of NPCs, global transcriptomal changes in transplanted NPCs, the effect of NPCs on neuroinflammation, and the causality between NPC transplantation and functional recovery. We found that NPCs transplanted following SCI differentiate mainly into oligodendrocytes and enhance myelination, upregulate genes related to synaptic signaling and mitochondrial activity, and downregulate genes related to cytokine production and immune system response. NPCs suppress the expression of pro-inflammatory cytokines/chemokines; moreover, NPC ablation confirm that NPCs were responsible for enhanced recovery in hindlimb locomotor function. Understanding the reaction of transplanted NPCs is important for exploiting their full potential. Existence of causality implies that NPCs are useful in the treatment of SCI.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery

et al. 2019

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“…A variety of cell types (e.g., Schwann cells, olfactory ensheathing cells, neural stem/ progenitor cells, and bone marrow stromal cells) have been transplanted into the injured spinal cord [10][11][12][13][14][15]. The goal of cellular transplantation is to replace the lost neural cells and reconnect the segmented spinal cord.…”

Section: Introductionmentioning

confidence: 99%

The Therapeutic Effectiveness of Delayed Fetal Spinal Cord Tissue Transplantation on Respiratory Function Following Mid-Cervical Spinal Cord Injury

et al. 2017

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Respiratory impairment due to damage of the spinal respiratory motoneurons and interruption of the descending drives from brainstem premotor neurons to spinal respiratory motoneurons is the leading cause of morbidity and mortality following cervical spinal cord injury. The present study was designed to evaluate the therapeutic effectiveness of delayed transplantation of fetal spinal cord (FSC) tissue on respiratory function in rats with mid-cervical spinal cord injury. Embryonic day-14 rat FSC tissue was transplanted into a C4 spinal cord hemilesion cavity in adult male rats at 1 week postinjury. The histological results showed that FSC-derived grafts can survive, fill the lesion cavity, and differentiate into neurons and astrocytes at 8 weeks post-transplantation. Some FSC-derived graft neurons exhibited specific neurochemical markers of neurotransmitter (e.g., serotonin, noradrenalin, or acetylcholine). Moreover, a robust expression of glutamatergic and γ-aminobutyric acid-ergic fibers was observed within FSC-derived grafts. Retrograde tracing results indicated that there was a connection between FSC-derived grafts and host phrenic nucleus. Neurophysiological recording of the phrenic nerve demonstrated that phrenic burst amplitude ipsilateral to the lesion was significantly greater in injured animals that received FSC transplantation than in those that received buffer transplantation under high respiratory drives. These results suggest that delayed FSC transplantation may have the potential to repair the injured spinal cord and promote respiratory functional recovery after mid-cervical spinal cord injury.

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“…Associated complications, however, are also rising, particularly in brain injuries (3). The incidence of neonatal brain injury occurs in >10% of all premature babies (4). Neonatal white matter injury (WMI) is a common type of brain injury in premature babies.…”

Section: Introductionmentioning

confidence: 99%

Effect of T helper cell 1/T helper cell 2 balance and nuclear factor-κB on white matter injury in premature neonates

Yuan

Cui

et al. 2018

Mol Med Report

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Incidence of white matter injury (WMI), which is featured as softening of white matter tissues, has recently increased. Previous studies have demonstrated a close correlation between T helper cell 1 and T helper cell 2 (Th1/Th2) imbalance and nuclear factor‑κB (NF‑κB) with brain disease. Their role in premature WMI, however, remains to be illustrated. Serum samples were collected from 60 premature WMI neonates, plus another control group of 60 premature babies without WMI. Patients were further divided into mild, moderate and severe WMI groups. Reverse transcription quantitative polymerase chain reaction was used to test mRNA expression levels of Th1/Th2 cytokines, including interleukin 2 (IL)‑2, tumor necrosis factor‑α (TNF‑α), IL‑4, IL‑10 and nuclear factor (NF)‑κB, whilst their serum levels were measured by ELISA. Their correlation with disease occurrence and progression were further analysed, to illustrate the effect of Th1/Th2 balance and NF‑κB on pathology of premature WMI. Serum levels of IL‑4 and IL‑10 were significantly decreased in premature WMI babies, whilst IL‑2, TNF‑α and NF‑κB were upregulated (P<0.05 vs. control group). With aggravated disease, IL‑4 and IL‑10 expression was further decreased while IL‑2, TNF‑α and NF‑κB were increased (P<0.05 vs. mild WMI group). Th1 cytokines IL‑2 and TNF‑α and NF‑κB were negatively correlated with Th2 cytokines IL‑4 and IL‑10. Disease severity was positively correlated with IL‑2, TNF‑α and NF‑κB expression, and was negatively correlated with IL‑4 and IL‑10 (P<0.05). Th1/Th2 imbalance and NF‑κB upregulation were observed in WMI pathogenesis, with elevated secretion of Th1 cytokines and decreased Th2 cytokines, suggesting that Th1/Th2 imbalance and NF‑κB upregulation may be a potential indicator for the early diagnosis and treatment of WMI pathogenesis and progression.

show abstract

Intraspinal transplantation of subventricular zone-derived neural progenitor cells improves phrenic motor output after high cervical spinal cord injury

Cited by 13 publications

References 58 publications

Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery

Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery

The Therapeutic Effectiveness of Delayed Fetal Spinal Cord Tissue Transplantation on Respiratory Function Following Mid-Cervical Spinal Cord Injury

Effect of T helper cell 1/T helper cell 2 balance and nuclear factor-κB on white matter injury in premature neonates

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