Intrathecal implantation surgical considerations in rodents; a review

Arman, Azim; Hutchinson, Mark R.

doi:10.1016/j.jneumeth.2021.109327

Cited by 2 publications

(3 citation statements)

References 53 publications

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“…It does not impose surgical stress on the study models and does not need the expensive indwelling catheter [1]. Placement of a catheter also induces immune reactions and has risk for spinal cord trauma, developing idiopathic hemorrhage or abscesses [9]. For single-shot injection, acute needle puncture is ideal, provided that tip placement can be confirmed.…”

Section: Discussionmentioning

confidence: 99%

“…Due to technical challenges requiring restraint methods like anesthesia, multiple puncture attempts might be required before successful insertion without any imaging aid, other drawback include a significant chance of damaging spinal or nerve roots. To address these challenges, imaging modality had been demonstrated in rodent models by Pasarikovski et al using real-time intraoperative imaging guidance like c-arm fluoroscopy [9,10]. The use of ultrasound demonstrated in this study provides visual guidance for needle puncture, reducing the number of attempts and providing evidences of successful intrathecal needle placement by visually ensuring that the needle tip remains in place during intrathecal administration.…”

Section: Discussionmentioning

confidence: 99%

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Ultrasound as a reliable guide for lumbar intrathecal injection in rats: A pilot study

Pryambodho,

Dilogo,

Tantri

et al. 2024

Preprint

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Lumbar intrathecal administration provides an ideal route for drug delivery into the central nervous system, especially when dorsal root ganglions are the main target for the therapy in rat model of chronic pain. Two main methods of lumbar intrathecal administrations are chronic catheter implantation and the acute needle puncture. Chronic catheter implantation involves surgical manipulation to insert micro indwelling catheter into the intrathecal space. However, this method is invasive, produces inflammatory reactions, and generates more surgical stress. Acute needle puncture is less invasive and cheaper but is technically challenging to perform. We performed an ultrasound-guided lumbar intrathecal injection in six male Sprague Dawley rat cadavers, on average weighing 250–300 grams. Fresh rat cadavers were positioned in a sternal recumbent position, vertebrae were palpated and scanned using a linear probe ultrasound. A 25G needle insertion was advanced with real-time ultrasound guidance, and placement was confirmed prior to dye injection (Methylene blue, Sigma Aldrich). Cadavers were then dissected, and the vertebrae were visually inspected for dye staining. All three cadavers that underwent intrathecal injection with sagittal and axial plane ultrasound guidance showed positive dye staining within the intrathecal space, confirming successful acute intrathecal administration. There was one successful intrathecal injection under sagittal plane-only ultrasound guidance. Ultrasound is a useful, operator-dependent tool to guide acute needle puncture intrathecal administration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ultrasound as a reliable guide for lumbar intrathecal injection in rats: A pilot study

Pryambodho,

Dilogo,

Tantri

et al. 2024

Preprint

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“…Following the European Directive 2010/63/EU (for review [ 12 ]) for the protection of laboratory animals and adhering to the 3Rs principle (replacement, reduction, and refinement) from The Principles of Humane Experimental Technique [ 13 ], in this work, we propose a new refined method to securely performed stereotaxic surgeries involving intracerebroventricular or intrathecal device implantation in long-term studies. These refinements focused on the main critical steps of intrathecal implantation in rodents (i.e., preoperative, intraoperative, and postoperative cares and procedures [ 14 ]) and were based on our experience in different studies conducted in our laboratory. Our proposed protocol introduces three significant changes.…”

Section: Introductionmentioning

confidence: 99%

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

Pérez-Martín¹,

Coto-Vilcapoma

Castilla-Silgado

et al. 2023

Animals

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Stereotaxic surgeries enable precise access to specific brain regions, being of particular interest for chronic intracerebroventricular drug delivery. However, the challenge of long-term studies at this level is to allow the implantation of drug storage devices and their correct intrathecal connection while guaranteeing animal welfare during the entire study period. In this study, we propose an optimized method for safe intrathecal device implantation, focusing on preoperative, intraoperative, and postoperative procedures, following the 3Rs principle and animal welfare regulations. Our optimized protocol introduces three main refinements. Firstly, we modify the dimensions of the implantable devices, notably diminishing the device-to-mouse weight ratio. Secondly, we use a combination of cyanoacrylate tissue adhesive and UV light-curing resin, which decreases surgery time, improves healing, and notably minimizes cannula detachment or adverse effects. Thirdly, we develop a customized welfare assessment scoresheet to accurately monitor animal well-being during long-term implantations. Taken together, these refinements positively impacted animal welfare by minimizing the negative effects on body weight, surgery-related complications, and anxiety-like behaviors. Overall, the proposed refinements have the potential to reduce animal use, enhance experimental data quality, and improve reproducibility. Additionally, these improvements can be extended to other neurosurgical techniques, thereby advancing neuroscience research, and benefiting the scientific community.

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Intrathecal implantation surgical considerations in rodents; a review

Cited by 2 publications

References 53 publications

Ultrasound as a reliable guide for lumbar intrathecal injection in rats: A pilot study

Ultrasound as a reliable guide for lumbar intrathecal injection in rats: A pilot study

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

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