Intrathecal lidocaine pretreatment attenuates immediate neuropathic pain by modulating Nav1.3expression and decreasing spinal microglial activation

Cheng, Kuang‐I; Lai, Chung-Sheng; Wang, Fuyuan; Wang, Hung-Chen; Chang, Lin‐Li; Ho, Shung‐Tai; Tsai, Hung-Pei; Kwan, Andy C.C.

doi:10.1186/1471-2377-11-71

Cited by 17 publications

(12 citation statements)

References 37 publications

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“…Lidocaine, a common local anesthetic in clinical practice, was diluted with 0.9% saline to the concentration of 100 μg/10 μl. Each rat was given 100 μg according to previous studies (Ma et al, 2003 ; Cheng et al, 2011 ). Ketamine, a noncompetitive N-methyl-D-aspartate receptor (NMDAR) antagonist for treating NP (Kozek et al, 2006 ), was diluted with 0.9% saline to the concentration of 10 μg/10 μl.…”

Section: Methodsmentioning

confidence: 99%

8-O-Acetyl Shanzhiside Methylester From Lamiophlomis Rotata Reduces Neuropathic Pain by Inhibiting the ERK/TNF-α Pathway in Spinal Astrocytes

Zhang

Bai

et al. 2018

Front. Cell. Neurosci.

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Lamiophlomis rotata (L. rotata; Benth.) Kudo is an effective traditional herb in the clinical treatment of chronic pain syndromes in China. 8-O-acetyl shanzhiside methylester (8-OaS), a chief component in L. rotata, possesses potent immunosuppressive activities and favorable analgesic effects. This study was proposed to compare the analgesic effects of 8-OaS with those of lidocaine and ketamine in a spinal nerve ligation (SNL) model by behavioral tests, and then investigated its effects upon the expression of spinal glial fibrillary acidic protein (GFAP), phosphorylated extracellular regulated protein kinases (pERK) and tumor necrosis factor-alpha (TNF-α) via immunofluorescence staining and western blot analyses. The data showed consecutive intrathecal injection of 8-OaS for 2 weeks brought about remarkable palliation of neuropathic pain (NP), possessing similar anti-allodynia effects with those of lidocaine and ketamine. Two weeks after surgery, pERK within the spinal dorsal horn was mainly expressed in astrocytes more than neurons and microglia, and 8-OaS inhibited spinal astrocytic activation and TNF-α expression. Finally, co-treatment of 8-OaS and PD98059 (an Extracellular signal-regulated kinase, ERK inhibitor) did not lead to remarkable increase in pain relief or TNF-α expression comparing to rats treated with 8-OaS or PD98059 alone. In conclusion, the anti-nociceptive effects of 8-OaS in the condition of NP relied on the inhibition of SNL-induced astrocyte activation, probably via the down-regulation of the ERK/TNF-α pathway.

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Section: Methodsmentioning

confidence: 99%

8-O-Acetyl Shanzhiside Methylester From Lamiophlomis Rotata Reduces Neuropathic Pain by Inhibiting the ERK/TNF-α Pathway in Spinal Astrocytes

Zhang

Bai

et al. 2018

Front. Cell. Neurosci.

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“…Paw withdrawal latency (PWL) was described previously to evaluate the thermal hyperalgesia (14). Thermal hyperalgesia was assessed by placing the hind paw on a radiant heat source and measuring the PWL under low-intensity heat set to a cut-off time of 30 seconds using an IITC analgesiometer (IITC360, Woodland Hills, CA).…”

Section: Assessment Ofneuropathic Pain and Behavioral Testingmentioning

confidence: 99%

Neuroprotective and Anti-Inflammatory Activities of Atorvastatin in a Rat Chronic Constriction Injury Model

Chu

Chen

et al. 2012

Int J Immunopathol Pharmacol

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The first two authors contributed equally to this work Atorvastatin is an HMG-CoA reductase inhibitor used to treat hypercholesterolemic conditions associated with hypertension. This study aims to investigate the anti-inflammatory and neuroprotective effects of atorvastatin on peripheral neuropathic pain. Peripheral neuropathic pain was induced by chronic constriction injury (CCI) in Sprague-Dawley rats. Rats were divided into 3 groups including shamoperated, CCI, and atorvastatin-treated. Atorvastatin (10 mg/kg) or phosphate-buffered saline was orally administered for 2 weeks. All animals were assessed by neurobehavioral tests before surgery and at days 3,7,14 after surgery. Inflammatory and neuroprotective factors were evaluated by Western blot analysis. eNOS, COX2 and iNOS in the sciatic nerve were also studied using immunohistochemistry. Atorvastatin attenuated CCI-induced nociceptive sensitization and thermal hyperalgesia in a time-dependent manner. Atorvastatin improved CCI-induced neurobehavioral/inflammatory activity by inhibition of TGF-p, pIKB/lKB, NFKB, COX2, iNOS, EP1 and EP4 in the sciatic nerve. Atorvastatin was also found to increase neuroprotection factors pAktlAkt, eNOS and VEGF. Taken together, these data indicate that atorvastatin could protect the sciatic nerve against CCI-induced neuroinflammation and nociception.Neuropathic pain, largely resulting from primary lesions in the peripheral nerve or from malfunctions in the central nervous system (CNS), has an extremely negative impact on the quality of life of patients affected by this condition (1).Studies using the chronic constriction injury (CCI) model of peripheral nerve injury have provided a deeper understanding of nociception and the events contributing to the pathogenesis of chronic pain conditions (2). Inflammatory states within the

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“…Blockade of early spontaneous afferent activity with the sodium channel blocker tetrodotoxin (TTX) following SNL also suppresses neuropathic pain [ 12 ]. The blockade of signaling by TTX was accompanied by reduced activation of satellite glial cells in the dorsal root ganglia (DRG), which could explain its analgesic effect as activated glial cells play a central role in neuropathic pain [ 54 , 55 ]. Ketamine exerts at least part of its analgesic effects through antagonism of the N-Methyl-D-aspartate (NMDA) receptor.…”

Section: Discussionmentioning

confidence: 99%

Preemptive perineural bupivacaine attenuates the maintenance of mechanical and cold allodynia in a rat spinal nerve ligation model

et al. 2015

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BackgroundNeuropathic pain is evasive to treat once developed, however evidence suggests that local administration of anesthetics near the time of injury reduces the development of neuropathic pain. As abnormal electrical signaling in the damaged nerve contributes to the initiation and maintenance of neuropathic pain, local administration of anesthetics prior to injury may reduce its development. We hypothesized that local treatment with bupivacaine prior to nerve injury in a rat model of spinal nerve ligation (SNL) would attenuate the initiation and/or maintenance of neuropathic pain behaviors.MethodsOn the day prior to SNL, baseline measures of pre-injury mechanical, thermal, and/or cold sensitivity were recorded in adult male Sprague–Dawley rats. Immediately prior to SNL or sham treatment, the right L5 nerve was perineurally bathed in either 0.05 mL bupivacaine (0.5 %) or sterile saline (0.9 %) for 30 min. Mechanical allodynia, thermal hyperalgesia, and/or cold allodynia were then examined at 3, 7, 10, 14 and 21 days following SNL.ResultsRats exhibited both mechanical and cold allodynia, but not thermal hyperalgesia, within 3 days and up to 21 days post-SNL. No significant pain behaviors were observed in sham controls. Preemptive local bupivacaine significantly attenuated both mechanical and cold allodynia as early as 10 days following SNL compared to saline controls and were not significantly different from sham controls.ConclusionsThese data indicate that local treatment with bupivacaine prior to surgical manipulations that are known to cause nerve damage may protect against the maintenance of chronic neuropathic pain.

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Intrathecal lidocaine pretreatment attenuates immediate neuropathic pain by modulating Nav1.3expression and decreasing spinal microglial activation

Cited by 17 publications

References 37 publications

8-O-Acetyl Shanzhiside Methylester From Lamiophlomis Rotata Reduces Neuropathic Pain by Inhibiting the ERK/TNF-α Pathway in Spinal Astrocytes

8-O-Acetyl Shanzhiside Methylester From Lamiophlomis Rotata Reduces Neuropathic Pain by Inhibiting the ERK/TNF-α Pathway in Spinal Astrocytes

Neuroprotective and Anti-Inflammatory Activities of Atorvastatin in a Rat Chronic Constriction Injury Model

Preemptive perineural bupivacaine attenuates the maintenance of mechanical and cold allodynia in a rat spinal nerve ligation model

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