Intrathecal Production of Specific Antibodies against Borrelia burgdorferi in Patients with Lymphocytic Meningoradiculitis (Bannwarth's Syndrome)

Wilske, Bettina; Schierz, G; Preac-Mursic, V.; Buscb, Käthe von; Kühbeck, R.; Pfister, Hans‐Walter; Einhäupl, Karl M.

doi:10.1093/infdis/153.2.304

Cited by 200 publications

(70 citation statements)

References 15 publications

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“…The two syphilis sera showed weak reactions: one with IgG against the proteins 4 = stages of the disease and in part also between clinical settings within the same stage we find considerable variations in number and nature of polypeptides bound and of binding isotypes. The number of polypeptides detected in this study is similar (16,17) or higher than in other reports (18)(19)(20). This might be due to several causes.…”

Section: Patientssupporting

confidence: 79%

“…Furthermore, we have shown that patients with stage I may exhibit distinct antibody patterns, depending on their clinical setting: sera of patients with ECM recognize the proteins 3 1 and 29 kD, but patients with headaches do not. The antibody pattern of the only child with meningitis is similar to that of reported patients (17,20). Despite divergences between the patients with different manifestations, we cannot discriminate a clear-cut antibody pattern for each setting in stage 11.…”

Section: Patientssupporting

confidence: 63%

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Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

1989

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ABSTRACT. We analyzed by means of immunoblot technique the patterns of antibodies binding to polypeptides of Borrelia burgdorferi B31 in the sera of 21 children with different stages of Lyme disease. All sera but one recognized the flagellar protein 41 kD and all but two the 83-kD protein. The number of proteins recognized rose from clinical stage I to stage 111. The polypeptides of the mol wt 55 and 31 kD were exclusively bound by IgM and the proteins 66, 58, 39, and 36 kD exclusively by IgG. Based on the number of proteins visualized by single sera, IgM was the predominant isotype in stages I and I1 peaking in stage 11, whereas in stage I11 IgG predominated. Considering the number of proteins recognized and the corresponding antibody isotype, a serologic differentiation between the three stages of the disease is feasible: within stage I and within stage I11 patients with different clinical signs had distinct antibody patterns. No clearcut pattern could be discriminated in stage I1 for patients with different settings. Immunoblotting yields a possible distinction between active infection and serological scar by the detection of specific antibody patterns. (Pediatr Res 26:377-382, 1989)

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Section: Patientssupporting

confidence: 79%

Section: Patientssupporting

confidence: 63%

Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

1989

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“…There are currently no FDA-approved tests to measure intrathecal production of antibodies in CSF. Methods that have been used to detect antibodies in CSF include capture immunoassays, CSF/ serum indices determined by ELISA, and Western immunoblots (69,113,364). Determination of intrathecal production of antibodies can be accomplished by measuring the CSF/ serum index of B. burgdorferi sensu lato antibodies.…”

Section: Immunologic Diagnosis Of B Burgdorferi Sensu Lato Infectionmentioning

confidence: 99%

Diagnosis of Lyme Borreliosis

et al. 2005

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A large amount of knowledge has been acquired since the original descriptions of Lyme borreliosis (LB) and of its causative agent, Borrelia burgdorferi sensu stricto. The complexity of the organism and the variations in the clinical manifestations of LB caused by the different B. burgdorferi sensu lato species were not then anticipated. Considerable improvement has been achieved in detection of B. burgdorferi sensu lato by culture, particularly of blood specimens during early stages of disease. Culturing plasma and increasing the volume of material cultured have accomplished this. Further improvements might be obtained if molecular methods are used for detection of growth in culture and if culture methods are automated. Unfortunately, culture is insensitive in extracutaneous manifestations of LB. PCR and culture have high sensitivity on skin samples of patients with EM whose diagnosis is based mostly on clinical recognition of the lesion. PCR on material obtained from extracutaneous sites is in general of low sensitivity, with the exception of synovial fluid. PCR on synovial fluid has shown a sensitivity of up to >90% (when using four different primer sets) in patients with untreated or partially treated Lyme arthritis, making it a helpful confirmatory test in these patients. Currently, the best use of PCR is for confirmation of the clinical diagnosis of suspected Lyme arthritis in patients who are IgG immunoblot positive. PCR should not be used as the sole laboratory modality to support a clinical diagnosis of extracutaneous LB. PCR positivity in seronegative patients suspected of having late manifestations of LB most likely represents a false-positive result. Because of difficulties in direct methods of detection, laboratory tests currently in use are mainly those detecting antibodies to B. burgdorferi sensu lato. Tests used to detect antibodies to B. burgdorferi sensu lato have evolved from the initial formats as more knowledge on the immunodominant antigens has been collected. The recommendation for two-tier testing was an attempt to standardize testing and improve specificity in the United States. First-tier assays using whole-cell sonicates of B. burgdorferi sensu lato need to be standardized in terms of antigen composition and detection threshold of specific immunoglobulin classes. The search for improved serologic tests has stimulated the development of recombinant protein antigens and the synthesis of specific peptides from immunodominant antigens. The use of these materials alone or in combination as the source of antigen in a single-tier immunoassay may someday replace the currently recommended two-tier testing strategy. Evaluation of these assays is currently being done, and there is evidence that certain of these antigens may be broadly cross-reactive with the B. burgdorferi sensu lato species causing LB in Europe

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“…In all of these disorders, the intrathecal IgG-antibody response is specific to the underlying infectious agent (17)(18)(19)(20)(21). Therefore, it is conceivable that the persistent IgG response in MS targets disease-relevant antigens.…”

Section: Introductionmentioning

confidence: 99%

Identification of Epstein-Barr virus proteins as putative targets of the immune response in multiple sclerosis

Cepok¹,

Zhou²,

Srivastava³

et al. 2005

J. Clin. Invest.

267

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MS is a chronic inflammatory and demyelinating disease of the CNS with as yet unknown etiology. A hallmark of this disease is the occurrence of oligoclonal IgG antibodies in the cerebrospinal fluid (CSF). To assess the specificity of these antibodies, we screened protein expression arrays containing 37,000 tagged proteins. The 2 most frequent MS-specific reactivities were further mapped to identify the underlying high-affinity epitopes. In both cases, we identified peptide sequences derived from EBV proteins expressed in latently infected cells. Immunoreactivities to these EBV proteins, BRRF2 and EBNA-1, were significantly higher in the serum and CSF of MS patients than in those of control donors. Oligoclonal CSF IgG from MS patients specifically bound both EBV proteins. Also, CD8 + T cell responses to latent EBV proteins were higher in MS patients than in controls. In summary, these findings demonstrate an increased immune response to EBV in MS patients, which suggests that the virus plays an important role in the pathogenesis of disease.

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Intrathecal Production of Specific Antibodies against Borrelia burgdorferi in Patients with Lymphocytic Meningoradiculitis (Bannwarth's Syndrome)

Cited by 200 publications

References 15 publications

Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

Diagnosis of Lyme Borreliosis

Identification of Epstein-Barr virus proteins as putative targets of the immune response in multiple sclerosis

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