Intrathecal transplantation of Wharton’s jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury

Mohamadi, Yousef; Moghahi, Seyed Mohammad Hossein Noori; Mousavi, Mahboubeh; Borhani-Haghighi, Maryam; Abolhassani, Farid; Kashani, Iraj Ragerdi; Hassanzadeh, Gholamreza

doi:10.1016/j.jchemneu.2019.01.011

Cited by 29 publications

(21 citation statements)

References 41 publications

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“…Because WJ-MSCs exert neuroprotective and neurotrophic effects, attempts have been made to also utilize them in spinal cord injury treatment. After the intrathecal transplantation of WJ-MSCs into rats with spinal cord injury, in several studies, the observed effects were similar, namely, an improved locomotor recovery and higher amount of spared gray matter compared to the controls [10,83,84]. Krupa et al [83] tested two dosages of cells (0.5 M and 1.5 M), which were administered one to three times in weekly intervals.…”

Section: Selected Animal Modelsmentioning

confidence: 99%

“…Compared to the treatment with stem cells, the use of culture medium improved axonal sprouting and reduced the number of reactive astrocytes; therefore, the use of CM instead of the cells themselves seems to be an attractive alternative for spinal injury treatment [84]. Mohamadi et al [10] focused on the molecular aspects of WJ-MSCs' anti-inflammatory potential. They found that stem cell administration caused a decrease in expression of inflammasome complex components, illustrating an anti-inflammatory potential of WJ-MSCs [10].…”

Section: Selected Animal Modelsmentioning

confidence: 99%

“…Mohamadi et al [10] focused on the molecular aspects of WJ-MSCs' anti-inflammatory potential. They found that stem cell administration caused a decrease in expression of inflammasome complex components, illustrating an anti-inflammatory potential of WJ-MSCs [10].…”

Section: Selected Animal Modelsmentioning

confidence: 99%

“…Recently, the perinatal tissues, such as the umbilical cord, have become of interest in regards to cellular therapies; because they are typically discarded after birth, their utilization as an MSC source is not ethically controversial and their collection does not involve a painful procedure. Moreover, it is suggested that MSCs from such neonatal tissues may have higher stemness potential than other MSCs [10]. Apart from this, contrary to bone marrow-derived MSCs (BM-MSCs), they do not exhibit contact-inhibited cell growth and their proliferation rate is higher than for BM-MSCs [11].…”

Section: Introductionmentioning

confidence: 99%

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Human Wharton’s Jelly—Cellular Specificity, Stemness Potency, Animal Models, and Current Application in Human Clinical Trials

Stefańska

Ożegowska

Hutchings

et al. 2020

JCM

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Stem cell therapies offer a great promise for regenerative and reconstructive medicine, due to their self-renewal and differentiation capacity. Although embryonic stem cells are pluripotent, their utilization involves embryo destruction and is ethically controversial. Therefore, adult tissues that have emerged as an alternative source of stem cells and perinatal tissues, such as the umbilical cord, appear to be particularly attractive. Wharton’s jelly, a gelatinous connective tissue contained in the umbilical cord, is abundant in mesenchymal stem cells (MSCs) that express CD105, CD73, CD90, Oct-4, Sox-2, and Nanog among others, and have the ability to differentiate into osteogenic, adipogenic, chondrogenic, and other lineages. Moreover, Wharton’s jelly-derived MSCs (WJ-MSCs) do not express MHC-II and exhibit immunomodulatory properties, which makes them a good alternative for allogeneic and xenogeneic transplantations in cellular therapies. Therefore, umbilical cord, especially Wharton’s jelly, is a promising source of mesenchymal stem cells.

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Section: Selected Animal Modelsmentioning

confidence: 99%

Section: Selected Animal Modelsmentioning

confidence: 99%

Section: Selected Animal Modelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human Wharton’s Jelly—Cellular Specificity, Stemness Potency, Animal Models, and Current Application in Human Clinical Trials

Stefańska

Ożegowska

Hutchings

et al. 2020

JCM

View full text Add to dashboard Cite

show abstract

“…Adipose tissue (AD)-derived MSCs were found to ameliorate chronic colitis by the reduction of total number of macrophages and M1 macrophages, mediated through the inhibitory action of PGE-2 on inflammasome complex formation in macrophages (10). In a rat spinal cord injury model, transplantation of Wharton’s jelly-derived MSCs was found to suppress the NMRP1 inflammasome and improve motor function recovery (11). Moreover, human umbilical cord (HUC)-derived MSCs have been shown to effectively reduce inflammatory responses through blocking NLRP3 inflammasome activation for therapies in type 2 diabetes (12).…”

Section: Introductionmentioning

confidence: 99%

Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages

Kim

et al. 2019

IJSC

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Background and ObjectivesPerivascular stem cells (PVCs) have been identified as precursors of mesenchymal stem cells (MSCs) that offer promising prospects for application in the development of cellular therapies. Although PVCs have been demonstrated to have greater therapeutic potential compared to bone marrow and adipose tissue-derived MSCs in various diseases, the regulatory role of PVCs on inflammasome activation during macrophage-mediated inflammatory responses has not been investigated.Methods and ResultsIn this study, we found that the PVC secretome effectively alleviates secretion of both caspase-1 and interleukin-1β in lipopolysaccharide-primed and activated human and murine macrophages by blocking inflammasome activation and attenuating the production of mitochondrial reactive oxygen species (ROS). We further showed that the PVC secretome significantly reduces inflammatory responses and endoplasmic reticulum stress in peritoneal macrophages in a mouse model of monosodium urate-induced peritonitis. A cytokine antibody array analysis revealed that the PVC secretome contains high levels of serpin E1 and angiogenin, which may be responsible for the inhibitory effects on mitochondrial ROS generation as well as on inflammasome activation.ConclusionsOur results suggest that PVCs may be therapeutically useful for the treatment of macrophage- and inflammation-mediated diseases by paracrine action via the secretion of various biological factors.

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Mesenchymal stem cells (MSCs) and MSC‐derived exosomes in animal models of central nervous system diseases: Targeting the NLRP3 inflammasome

et al. 2023

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The NLRP3 (NOD‐, LRR‐, and pyrin domain‐containing protein 3) inflammasome is a multimeric protein complex that is engaged in the innate immune system and plays a vital role in inflammatory reactions. Activation of the NLRP3 inflammasome and subsequent release of proinflammatory cytokines can be triggered by microbial infection or cellular injury. The NLRP3 inflammasome has been implicated in the pathogenesis of many disorders affecting the central nervous system (CNS), ranging from stroke, traumatic brain injury, and spinal cord injury to Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, and depression. Furthermore, emerging evidence has suggested that mesenchymal stem cells (MSCs) and their exosomes may modulate NLRP3 inflammasome activation in a way that might be promising for the therapeutic management of CNS diseases. In the present review, particular focus is placed on highlighting and discussing recent scientific evidence regarding the regulatory effects of MSC‐based therapies on the NLRP3 inflammasome activation and their potential to counteract proinflammatory responses and pyroptotic cell death in the CNS, thereby achieving neuroprotective impacts and improvement in behavioral impairments.

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Intrathecal transplantation of Wharton’s jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury

Cited by 29 publications

References 41 publications

Human Wharton’s Jelly—Cellular Specificity, Stemness Potency, Animal Models, and Current Application in Human Clinical Trials

Human Wharton’s Jelly—Cellular Specificity, Stemness Potency, Animal Models, and Current Application in Human Clinical Trials

Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages

Mesenchymal stem cells (MSCs) and MSC‐derived exosomes in animal models of central nervous system diseases: Targeting the NLRP3 inflammasome

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