Intratumoral genetic heterogeneity and number of cytogenetic aberrations provide additional prognostic significance in chronic lymphocytic leukemia

Abstract

The number of cytogenetic aberrations and the size of intratumoral genetic subclones should be comprehensively considered to determine the prognosis for CLL.Genet Med 19 2, 182-191.

The percentage of cells with 17p deletion and the size of 17p deletion subclones show prognostic significance in chronic lymphocytic leukemia

“…In accordance with that report, we defined 30% as a cutoff value to identify different cytogenetic clones and investigated whether intratumoral genetic subclones might partially contribute to the prognostic heterogeneity of patients with del(17p). Our results indicated that patients with a del(17p) as a minor clone had a survival advantage compared with patients with a del(17p) as a major clone, in agreement with prior studies . Therefore, integrating analyses of the size of intratumoral genetic subclones identified by FISH provides more precise prognostic stratification of CLL patients with del(17p).…”

“…The majority of patients (93%) with del(17p) harbored other cytogenetic abnormalities. Recently, Yi et al 23 reported that the sizes of intratumoral genetic subclones have significant impact on the prognosis of CLL. In accordance with that report, we defined 30% as a cutoff value to identify different cytogenetic clones and investigated whether intratumoral genetic subclones might partially contribute to the prognostic heterogeneity of patients with del(17p).…”

“…Our results indicated that patients with a del(17p) as a minor clone had a survival advantage compared with patients with a del(17p) as a major clone, in agreement with prior studies. 23,24 Therefore, integrating analyses of the size of intratumoral genetic subclones identified by FISH provides more precise prognostic stratification of CLL patients with del(17p).…”

Distinct clinical characteristics draw a new prognostic model for splenic marginal zone lymphoma in HBV high prevalent region

“…The DNA probes included the loci centromere 12 (CEP12), 13q14.3 (LSI D13S25 and RB-1), 14q32 (LSI IGHC/IGHV), 17p13 (LSI TP53), and 11q22 (LSI ATM). Sample preparations and hybridizations were conducted following the manufacturer's recommendations and as previously described [ 27 , 28 ]. IGH genes usage were detected Sanger sequencing as previous described [ 29 ].…”

Two Distinct <b><i>BCL2</i></b> Rearrangements, Each Observed in 2 Independent Subclones, Evolving from a Founder Clone with Trisomy 12 in a Unique Case of Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

“…Cytogenetic aberrations observed in CLL/SLL provide valuable prognostic information including trisomy 12 and the deletions 13q14.3, 6q21, 11q22-23 ( ATM ), and 17p13 ( TP53 ) [1,2,3,4,5,6]. Importantly, CLL/ SLL can also acquire cytogenetic aberrations that result in clonal evolution and disease progression [7,8].…”