Intratumoral microvessel density predicts local treatment failure of radically irradiated squamous cell cancer of the oropharynx

Aebersold, Daniel M.; Beer, Karl; Laissue, Jean A.; Hug, Stefan; Kollár, Attila; Greiner, Richard; Djonov, Valentin

doi:10.1016/s0360-3016(00)00573-3

Cited by 50 publications

(35 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For VEGF, a cutoff of 20% cytoplasmic staining in the tumor cells was used to distinguish low from high VEGF-expressing tumors, in order to be in consensus with the majority of the studies performed so far. [12][13][14] For Ki-67, nuclear staining was evaluated and a cutoff of 20% was used to separate tumors with low vs high mitotic rate, as described previously. 30,31 VEGF receptors are mainly expressed in tumor vasculature; 9 but their expression in cancer cells was also described.…”

Section: Antibody Expressionmentioning

confidence: 99%

“…9 Studies examining the association between VEGF immunohistochemical expression and patients' survival in head and neck cancer are more consensus than those examining microvessel density, pointing towards a correlation with poorer overall survival. [10][11][12][13][14][15][16] Also, many studies failed to show association of VEGF expression with higher microvessel density. 15,16 These aspects raise the possibility that VEGF, which is produced by neoplastic cells, might serve not only for vessel sprouting, but have other functions as well.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Potential autocrine function of vascular endothelial growth factor in head and neck cancer via vascular endothelial growth factor receptor-2

et al. 2005

View full text Add to dashboard Cite

Vascular endothelial growth factor is a peptide with well-defined actions on the vasculature and fundamental role in tumor angiogenesis. Its action in vascular endothelium is exerted in a paracrine manner. The immunohistochemical expression of this protein by cancer cells in head and neck squamous cell carcinoma was correlated with increased tumor aggressiveness and poor survival in previous studies. In the past years, an increasing amount of studies demonstrated potential autocrine action of vascular endothelial growth factor in various neoplasms. However, the existence and the impact of such autocrine action in head and neck cancer have not been demonstrated yet. In this retrospective study, we evaluated the expression of vascular endothelial growth factor and its receptors in neoplastic cells, in a cohort of patients with head and neck squamous cell carcinoma, and compared this expression with tumor aggressiveness, clinicopathologic parameters and outcome. High expression of vascular endothelial growth factor was strongly correlated with high expression of vascular endothelial growth factor receptor-2 (but not vascular endothelial growth factor receptor-1) on the cancer cells (Po0.001). The co-overexpression of both the protein and vascular endothelial growth factor receptor-2 was associated with higher tumor proliferation rate (Po0.001). The above cooverexpression also correlated with worse survival (log rank Po0.05) in patients with oral-larynx squamous cell carcinoma. Our results suggest that an autocrine vascular endothelial growth factor loop, mediated via vascular endothelial growth factor receptor-2, probably exists in head and neck squamous cell carcinoma. These observations support the hypothesis that the use of vascular endothelial growth factor receptor-2 inhibitors as adjuvant antiangiogenic therapy might have beneficial effects for these patients, by disrupting both paracrine (endothelial-dependent) and autocrine actions of vascular endothelial growth factor.

show abstract

Section: Antibody Expressionmentioning

confidence: 99%

mentioning

confidence: 99%

Potential autocrine function of vascular endothelial growth factor in head and neck cancer via vascular endothelial growth factor receptor-2

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Intratumoral microvessel density (IMD) represented a powerful, independent predictive factor for locoregional tumor control after radical irradiation for carcinoma of the oropharynx [24]. High vessel density was correlated with a worse prognosis than was scarce vascularization.…”

Section: Biologic Factors To Predict Treatment Resultsmentioning

confidence: 99%

Prognostic Factors in Squamous Cell Carcinoma of the Head and Neck

Wendt¹,

Bank²

2002

Oncol Res Treat

View full text Add to dashboard Cite

Therapy for squamous cell carcinoma of head and neck relies on surgery, radiotherapy and chemotherapy, mostly a combination thereof. In patients treated with curative intent, the intensity of therapy is adapted to the supposed prognosis and should be defined upon prognostic factors. Besides classical prognostic parameters, T, N and M stage, the presence of extranodal growth (extracapsular spread, ECS), tumor volume, lymph node burden, extent of tumor necrosis, histologic grading, but also type of treatment were determined in consideration of prognosis. The p53 status does not correlate with prognosis in most investigations. The tumor hypoxia seems to be of prognostic value, and strategies to overcome the adverse effect are currently investigated. Not all factors are relevant for all types of treatment. Besides ECS, these new factors so far have rarely been used to stratify prospective combined modality treatment according to the risk of locoregional and distant failure.

show abstract

“…Although there are substantial data implying that poorly vascularized tumors are resistant to chemotherapy and/or radiotherapy, no definitive conclusion has been drawn at present on the clinical usefulness of MVD as a marker for prognosis. There are some studies that did not show a relationship between MVD and survival (18,19) and a reverse relationship between MVD and survival has been reported by some groups (20,21).…”

Section: Discussionmentioning

confidence: 99%

Computer-Assisted Analysis of Biopsy Specimen Microvessels Predicts the Outcome of Esophageal Cancers Treated with Chemoradiotherapy

Zhang

Hironaka²,

Ohtsu³

et al. 2006

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: A computer-assisted microvessel analysis system was developed to evaluate correlations between the architecture of biopsy specimen microvessels and the outcome for patients with esophageal cancer treated with chemoradiotherapy. Experimental Design: Biopsy specimens from 51 patients with esophageal cancer (T 2-3 , any N, M 0 ) treated with chemoradiotherapy were immunostained with an anti-CD31 antibody and quantified using computerized image analysis.We evaluated the association of several microvessel factors with overall survival, including the ratio of total microvessel perimeter to total tumor area (TP/TA), the tumor hypoxic ratio, and the ratio of total microvessel number to total tumor area (TN/ TA). Results from traditional manual microvessel density (MVD) hotspot count and computerized hotspot count were compared and the relation between hotspot MVD count and survival rate was evaluated. Results: The median follow-up time was 32 months. Both univariate and multivariate analyses revealed that computer-counted hotspot MVD andTN/TA andTP/TA ratios correlated significantly with the outcome of chemoradiotherapy. Kaplan-Meier survival curves showed that patients with high computer-counted hotspot MVDs and highTN/TA andTP/TA ratios had better overall survival rate than patients with low MVDs or ratios (P = 0.025, 0.008, and 0.031, respectively). Combining computer-counted MVD orTN/TA ratio withTP/TA ratio proved more predictive than any single factor.Two researcher-counted hotspot MVDs had no significant relation with outcome. Conclusion: Computer-assisted tumor microvessel analysis is a powerful tool in predicting the outcome for patients with esophageal cancer treated with chemoradiotherapy because intraobserver and interobserver variability is minimized.

show abstract

Intratumoral microvessel density predicts local treatment failure of radically irradiated squamous cell cancer of the oropharynx

Cited by 50 publications

References 33 publications

Potential autocrine function of vascular endothelial growth factor in head and neck cancer via vascular endothelial growth factor receptor-2

Potential autocrine function of vascular endothelial growth factor in head and neck cancer via vascular endothelial growth factor receptor-2

Prognostic Factors in Squamous Cell Carcinoma of the Head and Neck

Computer-Assisted Analysis of Biopsy Specimen Microvessels Predicts the Outcome of Esophageal Cancers Treated with Chemoradiotherapy

Contact Info

Product

Resources

About