Intratumoural expression of IL-6/STAT3, IL-17 and FOXP3 immune cells in the immunosuppressive tumour microenvironment of colorectal cancer<i>Immune cells-positive for IL-6, STAT3, IL-17 and FOXP3 and colorectal cancer development</i>

Gulubova, Maya; Chonov, Dimitur Chavdarov; Ivanova, Koni; Hristova, Mariyna Kostova; Krasimirova-Ignatova, Maria Magdalena; Vlaykova, Tatyana

doi:10.1080/13102818.2022.2072765

Cited by 4 publications

(12 citation statements)

References 75 publications

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“…In a study with colorectal cancer patients, a positive correlation was found between STAT3 + cells in the TME and patient survival. The auhtors also showed that IL-6 + immune cells were found significantly more often in early-stage tumors than in later-stage tumors [68].…”

Section: Indirect Effectsmentioning

confidence: 87%

Effects of Exercise-Induced Changes in Myokine Expression on the Tumor Microenvironment

Gunasekara,

Clauss,

Bloch

2024

Sports Med Int Open

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In this narrative review, we summarize the direct and indirect effects that myokines have on the tumor microenvironment. We took studies of various cancer types and species into account. Systematic reviews and metananalysees that matched the search terms were also considered. We searched databases for six months. As a narrative approach was chosen, no data was analyzed or reanalyzed. The goal of this narrative review is to create an overview on the topic to identify research gaps and answer the questions wheather myokine expression may be relevant in cancer research in regard to the tumor microenvironment. Six commonly known myokines were chosen. We found strong links between the influence exercise has on interleukin-6, oncostatin M, secreted protein acidic and cysteine rich and irisin in the context of tumor progression and inhibition via interactions with the tumor microenvironment. It became clear, that the effects of myokines on the tumor microenvironment can vary and contribute to disease progression or regression. Interactions among myokines and immune cells must also be considered and require further investigation. To date, no study has shown a clear connection, while multiple studies suggest further investigation of the topic, similar to the effects of exercise on myokine expression.

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Section: Indirect Effectsmentioning

confidence: 87%

Effects of Exercise-Induced Changes in Myokine Expression on the Tumor Microenvironment

Gunasekara,

Clauss,

Bloch

2024

Sports Med Int Open

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“…The sections were counterstained by Mayer’s hematoxylin. The method has been described previously 32 . We chose tumor tissue sections that do not contain necrosis.…”

Section: Methodsmentioning

confidence: 99%

“…MSI was evaluated as has been previously described 31,32 . Samples that lack nuclear staining of lymphocytes, some stromal cells, or the nuclei of adjacent normal epithelial cells are considered as uninformative 37,38 .…”

Section: Methodsmentioning

confidence: 99%

“…Immunоhistochemistry for Mismatch Repair (MMR) Proteins (MLH1, MSH2, MSH6, and PMS2) MSI was evaluated as has been previously described. 31,32 Samples that lack nuclear staining of lymphocytes, some stromal cells, or the nuclei of adjacent normal epithelial cells are considered as uninformative. 37,38 Cancer biopsies were considered MSI when tumor cell nuclei were negative for one or more of the proteins MLH1, MSH2, MSH6, and PMS2.…”

Section: Quantitative Analysis Of Immunoreactivity In Tumor Tissue (T...mentioning

confidence: 99%

“…IL-6 + immune cells in CRC tissue have been described by a few authors 2,10,25,32 . As an indispensable regulatory signaling molecule, IL-6 mediates T-helper 17 (Th17) and T-regulatory (Treg) cell function in TME 2,33 .…”

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confidence: 99%

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Interleukin-6-Positive Immune Cells as a Possible New Immunologic Marker Associated With the Colorectal Cancer Prognosis

Gulubova,

Chonov,

Aleksandrova

et al. 2024

Applied Immunohistochemistry &Amp; Molecular Morphology

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Chronic inflammation creates tumor microenvironment (TME) that facilitates colorectal cancer (CRC) cell proliferation, migration, metastasis, and tumor progression. Interleukin-6 (IL-6) is a proinflammatory cytokine with a pleiotropic effect on CRC development. We aimed to evaluate IL-6 expression in tumor cells and in immune cells in TME, to assess the serum level and IL6 -174 G/C genotype distribution and to correlate the results with selected morphologic and clinical parameters that may add useful information in understanding the mechanisms of human CRC progression. A total of 153 patients with CRC were recruited in the current study. We assessed the IL-6 serum concentration through the ELISA method, the expression of IL-6 in tumor and in immune cells by immunohistochemical and double immunofluorescence staining, the MSI status by immunоhistochemistry for 4 mismatch repair (MMR) proteins, and the genotype distributions for IL6 -174G/C (rs1800795) single-nucleotide polymorphism through PCR-RFLP method. Our results showed that serum IL-6 level were increased in CRC patients as compared with healthy controls (P<0.0001), and in patients with cancers with advanced histologic type (type IV). However, the higher concentration (above the median of 55.71 pg/mL) was with borderline association with longer survival of the patients after surgical therapy (P=0.055, Log rank test). We also found that IL-6+ immune cells prevailed in the invasive front (IF) of tumors compared with the tumor stroma (TS) (P<0.0001). More IL-6+ cells were recruited in the tumors with less advanced histologic type (I+II), with stronger inflammatory infiltrate in the IF, in early pTNM stages (I+II), without lymph node and distant metastases and the higher levels of IL-6+ cells, especially in the IF, were associated with longer survival (P=0.012). The results of our study suggest that although the serum levels of IL-6 are higher in CRC, the increased IL-6+ cells in tumor microenvironment, both in the invasive front and in tumor stroma, as well as the higher serum levels are associated with good prognostic variables and longer survival of the patients mainly in the early stages of CRC.

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Microsatellite unstable colorectal cancers are associated with increased CD1a- and CD83-positive dendritic cell infiltration

Gulubova,

Aleksandrova,

Yovchev

et al. 2023

Biotechnology & Biotechnological Equipment

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Intratumoural expression of IL-6/STAT3, IL-17 and FOXP3 immune cells in the immunosuppressive tumour microenvironment of colorectal cancerImmune cells-positive for IL-6, STAT3, IL-17 and FOXP3 and colorectal cancer development

Cited by 4 publications

References 75 publications

Effects of Exercise-Induced Changes in Myokine Expression on the Tumor Microenvironment

Effects of Exercise-Induced Changes in Myokine Expression on the Tumor Microenvironment

Interleukin-6-Positive Immune Cells as a Possible New Immunologic Marker Associated With the Colorectal Cancer Prognosis

Microsatellite unstable colorectal cancers are associated with increased CD1a- and CD83-positive dendritic cell infiltration

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