Intrauterine growth restriction induced by maternal low protein diet causes long-term alterations of thymic structure and function in adult male rat offspring – CORRIGENDUM

Armengaud, Jean-Baptiste; Dennebouy, Zelie; Labes, Danny; Fumey, Catherine; Candotti, Fabio; Yzydorczyk, Catherine; Simeoni, Umberto

doi:10.1017/s0007114520003293

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used a rat model of IUGR (Swiss Veterinarian Animal Care committee-VD3050-31.01.2017) [ 103 ]. Pregnant rats were randomly allocated during gestation to a control diet (23% casein (version 0001 210 SAFE, Augy, France); CTRL group) or to an isocaloric low-protein diet (9% casein (version 0040), SAFE); IUGR group).…”

Section: Methodsmentioning

confidence: 99%

Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction

Simoncini

Coppola

Rocca

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Infants born after intrauterine growth restriction (IUGR) are at risk of developing arterial hypertension at adulthood. The endothelium plays a major role in the pathogenesis of hypertension. Endothelial colony-forming cells (ECFCs), critical circulating components of the endothelium, are involved in vasculo-and angiogenesis and in endothelium repair. We previously described impaired functionality of ECFCs in cord blood of low-birth-weight newborns. However, whether early ECFC alterations persist thereafter and could be associated with hypertension in individuals born after IUGR remains unknown. A rat model of IUGR was induced by a maternal low-protein diet during gestation versus a control (CTRL) diet. In six-month-old offspring, only IUGR males have increased systolic blood pressure (tail-cuff plethysmography) and microvascular rarefaction (immunofluorescence). ECFCs isolated from bone marrow of IUGR versus CTRL males displayed a decreased proportion of CD31+ versus CD146+ staining on CD45− cells, CD34 expression (flow cytometry, immunofluorescence), reduced proliferation (BrdU incorporation), and an impaired capacity to form capillary-like structures (Matrigel test), associated with an impaired angiogenic profile (immunofluorescence). These dysfunctions were associated with oxidative stress (increased superoxide anion levels (fluorescent dye), decreased superoxide dismutase protein expression, increased DNA damage (immunofluorescence), and stress-induced premature senescence (SIPS; increased beta-galactosidase activity, increased p16INK4a, and decreased sirtuin-1 protein expression). This study demonstrated an impaired functionality of ECFCs at adulthood associated with arterial hypertension in individuals born after IUGR.

show abstract

Section: Methodsmentioning

confidence: 99%