Intrauterine Infusion of Leukocyte Platelet-rich Plasma Enhances Fertility Outcomes in the Murine Model of Asherman\'s Syndrome

Abstract

Background: Platelet-rich plasma (PRP) contains many platelets and growth factors (GFs) that have been proven to promote wound healing, cell proliferation, and migration. The impact of leukocyte platelet-rich plasma (L-PRP) consequence in the murine model of Asherman's syndrome (AS) is considerable. Aim: To evaluate the effect of one dose of L-PRP in the murine model of AS. Materials and methods: Asherman mouse models were conducted by inserting the 25 gauge needle into the uterine horn through the lumen and stabilized for three estrous cycles. L-PRP (20 µL/horn) was injected into the uterine horn through the vagina of mice at the diestrus of the third estrous cycle. The function of the uterine was recorded at the eighth estrous cycle. Statistical analysis: Data were expressed as mean ± standard deviation (SD). Student's t-test and one-way analysis of variance (ANOVA) were performed by GraphPad 8.0. Results: L-PRP solution increases the number of uterine glands, increases the thickness of the endometrium, and expands the area of uterine lumen in the PRP group after one cycle of estrus. Besides that, using the L-PRP solution increased the rate of pregnancy in the mouse. Conclusion: One dose of L-PRP has a positive impact on improving uterine function in the murine model of AS. Clinical significance: This study provides a scientific basis for the potential application of the one dose-L-PRP in the clinical treatment of endometrial damage.