Intrauterine injection of human chorionic gonadotropin before embryo transfer significantly improves the implantation and pregnancy rates in in vitro fertilization/intracytoplasmic sperm injection: a prospective randomized study

Mansour, Ragaa; Tawab, Nevine A.; Kamal, O.; El-Faissal, Yahia; Serour, A.; Aboulghar, Mohamed; Serour, Gamal I.

doi:10.1016/j.fertnstert.2011.09.044

Cited by 115 publications

(143 citation statements)

References 21 publications

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“…hCG was shown having direct paracrine effects on the endometrium that precede its endocrine role of rescuing the corpus luteum, and regulates IGFBP-1 and prolactin in decidualized human endometrial stromal cells [51][52][53][54]. Here, hCG did not affect MFGE8 gene expression levels, consistent with previous results from our laboratory showing no change in primary human endometrial epithelial cells [31].…”

Section: Discussionsupporting

confidence: 91%

Human endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)

Sarhan¹,

Bocca²,

Yu³

et al. 2013

Cell Signal Traffic

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Objective: We have recently showed that MFGE8, a novel epithelial cell protein in the human endometrium, upregulated during the window of implantation. We hypothesized that MFGE8 may act as a key modulator of endometrial remodeling and trophoblast invasion. The aims of this study were (i) to investigate the in vitro regulation of human endometrial epithelial cells MFGE8 transcription, translation, and secretion by sex steroids and hCG; and (ii) to examine the possibility of MFGE8 secretion via microvesicles. Design: Experimental in vitro study using Ishikawa cells. Setting: University center. Interventions: Treatment with estradiol (E2), progesterone (P4), and human chorionic gonatropin (hCG). Main outcome measures: MFGE8 mRNA and protein expression, and identification of secreted microvesicles by mass spectrometry (MS) and immunoblotting. Results: E 2 , but not P4 or hCG, significantly upregulated MFGE8 mRNA expression. hCG significantly increased MFGE8 secretion. Microvesicels obtained after ultracentrifugation were visualized with atomic force microscopy ranging from ~100 to 200 nm. In addition to the expected 46 kD protein, the microvesicles contained a second form of secreted MFGE8 measuring ~30 kD which was confirmed by MS. Conclusions: We demonstrated (i) dual effects of E 2 and hCG on the regulation of MFGE8, and (ii) MFGE8 protein secretion in association with microvesicles. MFGE8 has the potential to modulate endometrial function and implantation via exocrine and/ or paracrine-autocrine effects. To the best of our knowledge, this is the first demonstration of microvesicular secretion of any regulatory protein by endometrial epithelial cells, providing initial evidence suggestive of microvesicular participation in cellular trafficking information in the non-pregnant and pregnant endometrium.

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Section: Discussionsupporting

confidence: 91%

Human endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)

Sarhan¹,

Bocca²,

Yu³

et al. 2013

Cell Signal Traffic

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“…However, since in the latter studies, both hCG and LIF were administered systemically, their direct local effect on the endometrium is not clear. On the other hand, when the uterine cavity was flushed with medium supplemented with hCG just before embryo transfer, the pregnancy rate as well as implantation rate were increased as compared to controls [60]. Although promising, it is yet to be determined whether local delivery before embryo transfer, of substances involved in the implantation process, increases implantation efficiency.…”

Section: The Endometriummentioning

confidence: 99%

Assessment and treatment of repeated implantation failure (RIF)

Simon

Laufer

2012

J Assist Reprod Genet

215

161

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Repeated implantation failure (RIF) is determined when embryos of good quality fail to implant following several in vitro fertilization (IVF) treatment cycles. Implantation failure is related to either maternal factors or embryonic causes. Maternal factors include uterine anatomic abnormalities, thrombophilia, non-receptive endometrium and immunological factors. Failure of implantation due to embryonic causes is associated with either genetic abnormalities or other factors intrinsic to the embryo that impair its ability to develop in utero, to hatch and to implant. New methods of time-lapse imaging of embryos and assessment of their metabolic functions may improve selection of embryos for transfer, and subsequent outcomes for IVF patients, as well as for those diagnosed with RIF. This review discusses the various causes associated with RIF and addresses appropriate treatments.

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“…It is being thought that severe military conflicts in Africa shorten the expected lifetime for more than 2 years. In general, WHO had calculated that 269 thousand people had died in 1999 due to the effect of wars and that loss of 8.44 million healthy years of life had occurred (2,3). Wars negatively affect the provision of health services.…”

Section: War and Women's Healthmentioning

confidence: 99%

“…Despite major advances in clinical and laboratory techniques of in vitro fertilization (IVF), fertility success rate is still around 30% per cycle (2). It is estimated that more than half of lost pregnancies are due to failure of embryo implantation (1).…”

Section: Introductionmentioning

confidence: 99%

“…Successful implantation, following IVF/ embryo transfer (IVF/ET) depends upon several factors, such as quality of the embryo and endometrial receptivity. One of the most important and well-known of these factors is human chorionic gonadotropin (hCG) hormone (1,2). hCG is one of the first hormones to be secreted by the embryo before implantation, and sends a message inducing growth of maternal blood vessel.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Intrauterine Injection of Human Chorionic Gonadotropin Before Frozen-Thawed Embryo Transfer on Implantation and Clinical Pregnancy Rate: A Randomized Controlled Trial

Hosseini¹,

Farzadi²,

Abdollahi³

et al. 2016

IJWHR

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Intrauterine injection of human chorionic gonadotropin before embryo transfer significantly improves the implantation and pregnancy rates in in vitro fertilization/intracytoplasmic sperm injection: a prospective randomized study

Cited by 115 publications

References 21 publications

Human endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)

Human endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)

Assessment and treatment of repeated implantation failure (RIF)

Effect of Intrauterine Injection of Human Chorionic Gonadotropin Before Frozen-Thawed Embryo Transfer on Implantation and Clinical Pregnancy Rate: A Randomized Controlled Trial

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