Intrauterine insemination enhances fertility of frozen semen in superovulated ewes

Armstrong, David T.; Evans, G.

doi:10.1530/jrf.0.0710089

Cited by 43 publications

(17 citation statements)

References 7 publications

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“…The present experiments were undertaken to compare the extent to which these oestrus-synchronizing agents, alone and in combination with two gonadotrophin preparations, PMSG and (Armstrong & Evans, 1984).…”

Section: Introductionmentioning

confidence: 99%

Reduction of sperm transport in ewes by superovulation treatments

Evans¹,

Armstrong²

1984

Reproduction

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At 24 h after insemination one uterine horn and one oviduct were flushed for recovery of spermatozoa and ova. When compared with naturally cyclic ewes, PG synchronization resulted in a marked reduction in the numbers of spermatozoa recovered (P < 0\m=.\05), and sponge synchronization led to a small, non-significant, reduction. Within the synchronized ewes, PMSG and FSH resulted in an equivalent superovulatory response, but there was a marked reduction in sperm recovery when compared with unstimulated animals (P < 0\m=.\01),with the greatest reduction attributable to PMSG treatment. Spermatozoa were recovered from fewer ewes treated with PMSG than with FSH (P < 0\m=.\05).Despite the observed impairment of sperm transport, a high fertilization rate was observed within each group and there were no differences between treatments.

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Section: Introductionmentioning

confidence: 99%

Reduction of sperm transport in ewes by superovulation treatments

Evans¹,

Armstrong²

1984

Reproduction

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“…The present experiments and data in the literature on the importance of sperm motility for invivo fertilization (Gould et ai, 1983;Hunter & Nichol, 1983;Armstrong & Evans, 1984) have shown why it is not logical to relate results from the sperm penetration assay with fertilizing ability in vivo. The zona-free hamster egg penetration assay appears to be more important as a useful method for the experimental study of the characteristics of gamete fusion and of the factors which support or inhibit it.…”

Section: Discussionmentioning

confidence: 66%

Some factors influencing the interaction of ram spermatozoa with zona-free hamster eggs

Pavlok¹,

Fléchon²

1985

Reproduction

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Variability in the interaction of ram spermatozoa with zona-free hamster eggs was recorded not only amongst individual males but also between the first and second ejaculates of the same male collected 30 min apart. Fusion ability also differed according to the conditions of gamete mixing. This ability decreased after in-vitro storage of undiluted ejaculates at room temperature but lasted for 48-192 h. The kinetics of sperm-egg fusion during the time of gamete incubation varied not only with the time of sperm storage in vitro but also with the ejaculate. When the semen was frozen, the ability of the spermatozoa to fuse was markedly reduced.

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“…Laparoscopy has been successfully used for insemination in deer (Asher et al., ; Dradjat, ), goats (Kulaksiz & Daskin, ; Vallet, Baril, Leboeuf, & Perin, ) and sheep (Gourley & Riese, ; Hiwasa, Kohno, Togari, Okabe, & Fukui, ), generating pregnancy rates over 70% with fresh semen and 50% using frozen semen. The technique has also been used to inseminate superovulated ewes with conventional (Armstrong & Evans, ) and SS (de Graaf et al., ) generating over 75% fertilization rates. This could suggest the possibility of using a laparoscopic insemination technique is cattle, particularly when SS is used and fertility results have been shown to be considerably low compared to the conventional insemination technique (DeJarnette et al., ).…”

Section: Discussionmentioning

confidence: 99%

A new device to inseminate cows at the base of the uterine horns

García-Peña

Rangel-Santos

Lara

et al. 2016

Reprod Domestic Animals

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A new device (Chapingo device) to deposit semen at the base of the uterine horns of cattle was developed at Universidad Autonoma Chapingo, Mexico. Nine Holstein heifers were inseminated by transvaginal laparoscopy, using a laparoscope for cattle and the Chapingo device. A dose of sexed semen (2.1 × 10 spermatozoa) was deposited at the base of the uterine horn ipsilateral to the ovary where the preovulatory follicle was identified. Insemination was achieved in all the heifers, taking on average 13.7 ± 3.1 min per animal. In all cases, it was possible to see both ovaries, the base of the uterine horns and the oviducts. After the procedure, none of the heifers showed any type of complications such as haemorrhage, adhesions or trauma. On days 21 and 22 after insemination, four of the nine heifers (44.4%) returned into oestrus; on day 30 after insemination, one heifer was found to be pregnant by ultrasound. The results show the feasibility of generating pregnancies by transvaginal laparoscopy in heifers inseminated with sexed semen.

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Intrauterine insemination enhances fertility of frozen semen in superovulated ewes

Cited by 43 publications

References 7 publications

Reduction of sperm transport in ewes by superovulation treatments

Reduction of sperm transport in ewes by superovulation treatments

Some factors influencing the interaction of ram spermatozoa with zona-free hamster eggs

A new device to inseminate cows at the base of the uterine horns

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