Intravaginal treatment with Marantodes pumilum (Kacip Fatimah) ameliorates vaginal atrophy in rats with post-menopausal condition

Tan, Nur; Giribabu, Nelli; Karim, Kamarulzaman; Nyamathulla, Shaik; Salleh, Naguib

doi:10.1016/j.jep.2019.02.027

Cited by 7 publications

(2 citation statements)

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“…The observed uterotonic action of MPE could be attributed to the presence of bioactive compounds in the extract. We recently published a LCMS/MS data analysis which showed that the same extract contains decarboxylated gallic acid (pyrogallol), gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, vanillic acid, myricetin, kaempferol-3-O-rutinoside, kaempferol-7-O-glucoside, 3-3-Di-O-methyellagic acid, apigenin and phytol (Tan et al, 2019). Pyrogallol has been reported to affect prostaglandin synthesis in the rat and human uterus (Kelly and Abel, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, other parts of M. pumilum such as roots and stems have also been used as medication, often in the form of decoction (Ibrahim and Jaafar, 2011). Previously, our study has shown that the leaves of M. pumilum contain high levels of phenolic acids, flavanoids and diterpenoid which might contribute to its health beneficial effects (Tan et al, 2019).…”

Section: Introductionmentioning

confidence: 87%

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Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period

Omar

Giribabu

Karim

et al. 2019

Journal of Ethnopharmacology

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Ethnopharmacological relevance: Marantodes pumilum (Blume) Kuntze has traditionally been used to firm the uterus after delivery, however scientific evidences behind this claim is still lacking. Aims of study: To demonstrate Marantodes pumilum leaves aqueous extract (MPE) has an effect on uterine contraction after delivery and to elucidate the molecular mechanisms involved. Methods: Day-1 post-delivery female rats were given MPE (100, 250 and 500 mg/kg/day) orally for seven consecutive days. A day after the last treatment (day-8), rats were sacrificed and uteri were harvested and subjected for ex-vivo contraction study using organ bath followed by protein expression and distribution study by Western blotting and immunohistochemistry techniques, respectively. The proteins of interest include calmodulin-CaM, myosin light chain kinase-MLCK, sarcoplasmic reticulum Ca 2+-ATPase (SERCA), G-protein α and β (Gα and Gβ), inositol-triphosphate 3-kinase (IP3K), oxytocin receptor-OTR, prostaglandin (PGF)2α receptor-PGFR, muscarinic receptor-MAChR and estrogen receptor (ER) isoforms α and β. Levels of estradiol and progesterone in serum were determined by enzyme-linked immunoassay (ELISA). Results: Ex-vivo contraction study revealed the force of uterine contraction increased with increasing doses of MPE. In addition, expression of CaM, MLCK, SERCA, Gα, Gβ, IP3K, OTR, PGF2α, MAChR, Erα and ERβ in the uterus increased with increasing doses of MPE. Serum analysis indicate that estradiol levels decreased while progesterone levels remained low at day-8 post-partum in rats receiving 250 and 500 mg/kg/day MPE. Conclusions: These findings support the claims that MPE help to firm the uterus and pave the way for its use as a uterotonic agent after delivery.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%