Intravenous acetylsalicylic acid, magnesium and their combination in experimental arterial thrombosis in rats

Fuglsang, Jens; Hb, Ravn; Ge, Toft; Thorwest, Martin; Husted, Søren; Ve, Hjortdal

doi:10.1097/00001721-199909000-00005

Blood Coagulation & Fibrinolysis

1999

DOI: 10.1097/00001721-199909000-00005

|View full text |Cite

Intravenous acetylsalicylic acid, magnesium and their combination in experimental arterial thrombosis in rats

Jens Fuglsang

Ravn Hb

Toft Ge

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1999

2002

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heparin-saline is often used topically during microvascular procedures. 2 However, other antithrombotic drugs, including magnesium, 3,4 aspirin, and tissue factor (TF) pathway inhibitor, 5 have been evaluated in animal models of microvascular surgery. An overview of antithrombotic agents in animal experiments was reported by Cooley and Gould.…”

mentioning

confidence: 99%

Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

et al. 1999

View full text Add to dashboard Cite

Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.

show abstract

mentioning

confidence: 99%

Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

et al. 1999

View full text Add to dashboard Cite

show abstract

Local heparin is superior to systemic heparin in preventing arterial thrombosis

2002

View full text Add to dashboard Cite

Thrombosis poses a significant problem in microvascular surgery, despite antithrombotic therapy. The purpose of the present study was to investigate whether a topical application of unfractionated heparin is equally efficient as a systemic bolus in avoiding thrombosis. A rat femoral artery model was used. Three different doses of systemic heparin (50, 100, and 200 U/kg) and one dose of locally administered heparin (100 U/ml) were evaluated and compared to a control group receiving isotonic saline. A thrombogenic injury, simulating poor microsurgical technique, was applied to the artery. The thrombus area was visualized by transillumination, and recorded for 60 min on video, for subsequent measurement. In addition, the level of activated partial thromboplastin time (APTT) and of anti-activated clotting factor X (aXa) was determined. Local heparin significantly reduced thrombus size as compared to isotonic saline and systemic heparin (50 and 100 U/kg). High-dose systemic heparin (200 U/kg) was equally potent, but local heparin had significantly less influence on the hemostatic parameters.

show abstract

Reactive Thrombocytosis Alone Does Not Affect the Patency of Microvascular Anastomosis in the Splenectomy Rat

Kuo¹,

Yang²,

Yang³

et al. 2002

Plastic and Reconstructive Surgery

View full text Add to dashboard Cite

Vascular thrombosis is a harbinger of failure in microsurgery. However, there is still controversy regarding the correlation of the complications of thrombocytosis and thrombosis. Some evidence indicates that patients with elevated platelet counts tend to have a higher flap failure rate, and surgeons usually hesitate to operate on patients with thrombocytosis. Nevertheless, the authors have experienced successful free tissue transfer in seven patients with thrombocytosis resulting from traumatic splenectomy or multiple trauma. On the basis of clinical observation, the authors investigated whether reactive thrombocytosis contributes to the patency of a microvascular anastomosis. In a rodent splenectomy-induced thrombocytosis model (n = 40), stable reactive thrombocytosis occurred after postoperative days 5 to 10, with the peak on postoperative day 7. Femoral artery division and reanastomosis was performed in rats with or without splenectomy-induced thrombocytosis, and vascular patency was assessed. Platelet counts and platelet activation were studied in correlation to microvascular patency. Platelet activation as demonstrated by CD62P expression on platelets was not significantly different between rats with and without thrombocytosis (6.41 +/- 0.95 percent versus 4.51 +/- 0.55 percent, respectively; p = 0.089). As immature platelets were not increased (2.86 +/- 0.33 percent versus 1.99 +/- 0.32 percent, p = 0.074), it seems that the splenectomy-induced thrombocytosis is the result of redistribution of platelets instead of an increase in bone marrow production. There were no significant differences in the patency rates or perfusion units of femoral artery after arterial anastomosis between rats with and without thrombocytosis (90 percent and 95 percent, respectively; p = 0.561). In conclusion, this study demonstrates that microvascular anastomosis can be performed safely in patients with reactive thrombocytosis without platelet activation.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Intravenous acetylsalicylic acid, magnesium and their combination in experimental arterial thrombosis in rats

Cited by 3 publications

References 0 publications

Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

Local heparin is superior to systemic heparin in preventing arterial thrombosis

Reactive Thrombocytosis Alone Does Not Affect the Patency of Microvascular Anastomosis in the Splenectomy Rat

Contact Info

Product

Resources

About