Intravenous iron administration induces oxidation of serum albumin in hemodialysis patients

Anraku, Makoto; Kitamura, Kenichiro; Shinohara, Ayumi; Adachi, Masataka; Suenaga, Atsushi; Maruyama, Toru; Miyanaka, Kei; Miyoshi, Takuji; Shiraishi, Naoki; Nonoguchi, Hiroshi; Otagiri, Masaki; Tomita, Kimio

doi:10.1111/j.1523-1755.2004.00813.x

Cited by 100 publications

(92 citation statements)

References 42 publications

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“…We monitored the ratio of oxidized to unoxidized albumin as a marker for oxidative stress in the systemic circulation due to its simplicity, rapidity and sensitivity for evaluating oxidative stress in vivo because the oxidation of albumin accounts for almost all of the excess plasma protein oxidation in uremic patients. In fact, we previously demonstrated that intravenous iron induced oxidative stress in hemodialysis patients by this technique (16).…”

Section: Discussionmentioning

confidence: 99%

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An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

Shimoishi

Anraku²,

Kitamura³

et al. 2007

Pharm Res

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104

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Purpose. The effect of AST-120, an oral adsorbent, on oxidative stress in the systemic circulation in chronic renal failure (CRF) was examined and the potential role of indoxyl sulfate (IS), an uremic toxin adsorbed by AST-120, in inducing the formation of reactive oxygen species (ROS) in the vascular system was studied, in vitro and in vivo. Materials and methods. The level of oxidized albumin, a marker for oxidative stress in the systemic circulation was determined by HPLC, as previously reported. The mRNA levels of TGF-b 1 and Oat1 were measured by quantitative RT-PCR. The IS induced ROS generation in cultured human umbilical vein endothelial cells (HUVECs) was estimated using a fluorescence microplate reader.Results. An increase in the ratio of oxidized to unoxidized albumin was determined using 5/6 nephrectomized rats (CRF rats) compared to a control group. The ratio was significantly reduced in the group that received AST-120 of 4 weeks, suggesting that AST-120 inhibits oxidative stress in CRF. An anti-oxidative effect of AST-120 was also observed in CRF rats with a similar renal function. The ratio of oxidized albumin was correlated with serum IS levels in vivo. The same relationship was also observed in CRF rats with the continued administration of IS. In addition, IS dramatically increased the generation of ROS in both a dose-and time-dependent manner in HUVEC, suggesting that accumulated IS may play an important role in enhancing intravascular oxidative stress. Conclusion. We propose that AST-120 reduces IS concentrations in the blood that induces ROS production in endothelial cells, thereby inhibiting the subsequent occurrence of oxidative stress in the systemic circulation in renal failure.

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Section: Discussionmentioning

confidence: 99%

“…Oxidative stress was evaluated by monitoring oxidized serum albumin levels, a sensitive marker for protein oxidation in the systemic circulation (16). Furthermore, we investigated the effect of IS on ROS production in endothelial cells to verify the mechanism of the antioxidant activity of AST-120 in CRF.…”

Section: Introductionmentioning

confidence: 99%

An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

Shimoishi

Anraku²,

Kitamura³

et al. 2007

Pharm Res

Self Cite

104

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“…27 Thiol concentrations in plasma were measured by means of the Ellman's method. 28 Reaction mixtures were prepared by adding 20 ml of plasma to 100 ml of 5 mM 5, 5 0 -dithiobis (2-nitrobenzoic acid) in 100 mM potassium phosphate (pH 7.0).…”

Section: Determination Of Thiol Contentmentioning

confidence: 99%

“…27 Frozen plasma samples obtained from healthy volunteers and heterozygotic FAP ATTR V30M patients were thawed, and then 5 ml aliquots were analyzed with a Shodex Asahipak ES-502N column (Showa Denko, Tokyo, Japan). The values of the reduced form (HMA) and the oxidized form (HNA) of plasma albumin were estimated from HPLC chromatograms by dividing the area of each fraction by the total area corresponding to albumin.…”

Section: Chromatography Of Oxidized Albuminmentioning

confidence: 99%

Loss of functional albumin triggers acceleration of transthyretin amyloid fibril formation in familial amyloidotic polyneuropathy

Kugimiya

Jono

Saito

et al. 2011

Laboratory Investigation

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Transthyretin (TTR)-related familial amyloidotic polyneuropathy (FAP) is characterized by systemic accumulation of amyloid fibrils caused by a point mutation in the TTR gene. Despite the urgent need for alternative therapeutic strategies, the pathogenesis of FAP still remains elusive. In our study reported here, we focused on albumin, the most abundant protein in plasma, and described the role of albumin in the TTR amyloid-formation process. Patients with FAP evidenced significantly decreased serum albumin levels as the disease progressed. Biacore analysis showed that albumin had a binding affinity for TTR and exhibited higher affinity for TTR amyloid than native TTR. Albumin functioning as an antioxidant effectively suppressed TTR amyloid formation. In patients with FAP, albumin was significantly oxidized as the disease progressed. Moreover, loss of functional albumin accelerated TTR deposition in analbuminemic rats possessing a human variant TTR gene. Taken together, these results indicate that albumin may have an inhibitory role in the TTR amyloid-formation process.

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“…The aim of this study was to examine the possible antioxidant and free radical-scavenging properties of olmesartan in in vitro studies. We also evaluated the effect of once daily administration of 40 mg of olmesartan on BP and on oxidized serum albumin, a marker of protein oxidation, in HD patients (16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients

et al. 2007

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Intravenous iron administration induces oxidation of serum albumin in hemodialysis patients

Cited by 100 publications

References 42 publications

An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

Loss of functional albumin triggers acceleration of transthyretin amyloid fibril formation in familial amyloidotic polyneuropathy

Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients

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