Intravesical Injection of Abobotulinumtoxin-A in Patients with Bladder Pain Syndrome/Interstitial Cystitis

Rahnama’i, Mohammad-Sajjad; Salehi‐Pourmehr, Hanieh; Saeedi, Sana; Tayebi, Sona; Hajebrahimi, Sakineh

doi:10.5152/tud.2023.22243

Cited by 1 publication

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“…In the urinary bladder, mast cells have been implicated in the pathogenesis of various disorders, such as overactive bladder [ 9 , 10 ], bladder outlet obstruction [ 11 , 12 ], urinary tract infections [ 13 ], bladder carcinoma [ 14 ] and various forms of cystitis, namely interstitial cystitis [ 10 , 15 , 16 ], radiation-induced cystitis [ 17 ] and bacterial cystitis [ 18 ]. In such pathologies, abnormalities have been reported in their activation state, prevalence and distribution.…”

Section: Introductionmentioning

confidence: 99%

Mast cell distribution and prevalence in the murine urinary bladder

Smith,

Tan,

Moro

2024

BMC Urol

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Background Mast cells have been implicated in the pathology of various urinary bladder disorders. However, the distribution of mast cells throughout urinary bladder tissue remains uncertain despite mast cell prevalence being relatively well-defined. Using a mouse tissue model, this study aims to characterise the prevalence and distribution of mast cells throughout the urinary bladder. Methods Bladder tissues were collected from six C57BL/6J female mice. Mast cell prevalence was quantified by flow cytometry, based on the expression of the following characteristic markers: CD45, CD117 and FcɛRIα. The toluidine blue stain assessed mast cell distribution, size, and proximity to vasculature. A repeated measures one-way ANOVA was used to evaluate the density of mast cells between the discrete layers of the urinary bladder, and an ordinary one-way ANOVA was used to assess potential differences between mast cell size across the urinary bladder wall. Results It was determined that mast cells compose less than 4% of all live leukocytes in the urinary bladder. They were also found to be more prominent in the lamina propria and detrusor muscle layers, compared to the urothelium and adventitia. In addition, 20.89% of mast cells were located near vasculature, which may be an important factor in consideration of their function and potential to contribute to various bladder pathologies, such as cystitis or overactive bladder. Conclusion These findings provide a baseline understanding of mast cell prevalence and distribution throughout the urinary bladder.

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