Intravesical treatment with cis-urocanic acid improves bladder function in rat model of acute bladder inflammation

Konkol, Yvonne; Bernoulli, Jenni; Streng, Tomi; Jääskeläinen, Katarina; Laihia, Jarmo K.; Leino, Lasse

doi:10.1002/nau.22818

Cited by 11 publications

(9 citation statements)

References 22 publications

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“…The second was an ovalbumin-induced ocular allergy model in which cis-UCA, like olopatadine, could significantly inhibit conjunctival vascular leakage. For treatment of acute hydrochloride acideinduced bladder inflammation, 2% cis-UCA as an intravesical infusion was as efficient as a proven treatment, 0.5% hyaluronic acid, in improving bladder function in rats (Konkol et al, 2016). Histopathological studies suggested that the anti-inflammatory actions of cis-UCA were due to its tissueprotective properties in reducing the detrimental activity of acute phase immune cells already present in the bladder, without affecting the infiltration of inflammatory cells.…”

Section: Introductionmentioning

confidence: 94%

The Multiple Roles of Urocanic Acid in Health and Disease

Hart

Norval

2021

Journal of Investigative Dermatology

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Section: Introductionmentioning

confidence: 94%

The Multiple Roles of Urocanic Acid in Health and Disease

Hart

Norval

2021

Journal of Investigative Dermatology

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“…Despite the fact that the effects of cis-UCA may be cell type-dependent, many in vivo and in vitro studies have revealed its anti-inflammatory properties and potential to protect from cell injury. [25][26][27][28] Our previous studies have revealed that cis-UCA is well tolerated by human corneal epithelial (HCE) and conjunctival epithelial (HCEC) cells. 29 Additionally, pretreatment with cis-UCA prevents cell death and the secretion of IL-8 and IL-6 in UV-B-induced HCE and HCEC cells.…”

mentioning

confidence: 99%

UV-B-Induced Inflammasome Activation Can Be Prevented by Cis-Urocanic Acid in Human Corneal Epithelial Cells

Korhonen

Bisevac

Hyttinen

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. The cornea is continually exposed to highly energetic solar UV-B (280-320 nm). Our aim was to investigate whether UV-B triggers the activation of NLRP3 inflammasomes and the production of IL-1β and/or IL-18 in human corneal epithelial (HCE) cells. Additionally, we studied the capability of cis-urocanic acid (cis-UCA) to prevent inflammasome activation or alleviate inflammation through other signaling pathways. METHODS. HCE-2 cell line and primary HCE cells were primed using lipopolysaccharide or TNF-α. Thereafter, cells were exposed to UV-B before or after the addition of cis-UCA or caspase-1 inhibitor. Caspase-1 activity was measured from cell lysates by an enzymatic assay. IL-1β, IL-18, IL-6, IL-8, and NLRP3 levels were detected using the ELISA method from cell culture media. Additionally, intracellular NLRP3 levels were determined by the Western blot technique, and cytotoxicity was measured by the LDH assay. RESULTS. UV-B exposure significantly increased caspase-1 activity in TNF-α-primed HCE cells. This result was consistent with the concurrently induced IL-1β secretion. Both caspase-1 activity and release of IL-1β were reduced by cis-UCA. Additionally, UV-B stimulated the caspase-1-independent production of IL-18, an effect also reduced by cis-UCA. Cis-UCA decreased the release of IL-6, IL-8, and LDH in a time-dependent manner when administered to HCE-2 cells after UV-B exposure. CONCLUSIONS. Our findings demonstrate that UV-B activates inflammasomes in HCE cells. Cis-UCA can prevent the secretion of IL-1β and IL-18 and therapeutically reduces the levels of IL-6, IL-8, and LDH in UV-B-stressed HCE cells.

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“…At present, animal models of cystitis are mainly generated by injecting toxic irritants into the bladder of the animals. The toxic irritants include hydrochloric acid, acetic acid, acetone, allyl aldehyde, lipopolysaccharide, protamine sulfate and cyclophosphamide, among which hydrochloric acid is a relatively simple and stable agent that can be used to induce chemical cystitis [22,30]. Previous studies have demonstrated that HCl-induced chemical cystitis in SD rats causes apparent bladder mucosal injury, increased urination time and increased bladder-related pain [23].…”

Section: Discussionmentioning

confidence: 99%

“…This method refers to the previously published protocol of Konkol et al [22]. The animals were anesthetized with 4% chloral hydrate (4% chloral hydrate/rat body weight = 1 ml/100 g).…”

Section: Methods Of Drug Administrationmentioning

confidence: 99%

The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats

Chen

Gao

et al. 2021

Int Urol Nephrol

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Purpose To evaluate the effects of manganese superoxide dismutase (Mn-SOD) from thermophilic bacterium HB27 (name as Tt-SOD) on chemical cystitis. Methods Control and experimental rats were infused by intravesical saline or hydrochloric acid (HCl) on the first day of the experiments. Saline, sodium hyaluronate (SH) or Tt-SOD were infused intravesically once a day for three consequent days. On the fifth day, the rats were weighted and sacrificed following a pain threshold test. The bladder was harvested for histological and biochemical analyses. Results Tt-SOD could reduce the bladder index, infiltration of inflammatory cells in tissues, serum inflammatory factors and SOD levels, mRNA expression of inflammatory factors in tissues, and increase perineal mechanical pain threshold and serum MDA and ROS levels in HCl-induced chemical cystitis. Furthermore, Tt-SOD alleviated inflammation and oxidative stress by the negative regulation of the NF-κB p65 and p38 MAPK signaling pathway. Conclusions Intravesical instillation of Tt-SOD provides protective effects against HCl-induced cystitis.

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Intravesical treatment with cis-urocanic acid improves bladder function in rat model of acute bladder inflammation

Cited by 11 publications

References 22 publications

The Multiple Roles of Urocanic Acid in Health and Disease

The Multiple Roles of Urocanic Acid in Health and Disease

UV-B-Induced Inflammasome Activation Can Be Prevented by Cis-Urocanic Acid in Human Corneal Epithelial Cells

The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats

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