Intravital imaging reveals cell cycle-dependent satellite cell migration during muscle regeneration

Konagaya, Yumi; Takakura, K.; Sogabe, Maina; Bisaria, A. K.; Liu, Chad; Meyer, Tobias; Sehara-Fujisawa, Atsuko; Terai, Kenta

doi:10.1101/2020.05.22.111138

Cited by 2 publications

(2 citation statements)

References 72 publications

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“…In Several studies have performed intravital imaging of muscle tissue [24,[49][50][51], however, only two of them dynamically monitored the process of muscle regeneration [24,49]. These two studies focused on the progenitor population by labelling PAX7 + cells, but they did not report on the dynamics of differentiation.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Benavente-Diaz

Comai

Girolamo

et al. 2020

Preprint

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Background: Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during differentiation. Results: Here, we report a knock-in mouse line expressing the tdTOMATO fluorescent protein from the endogenous Myogenin locus. Expression of tdTOMATO in MyogntdTom mice recapitulated endogenous Myogenin expression during embryonic muscle formation and adult regeneration and enabled the isolation of the Myogenin+ cell population. We also show that tdTOMATO fluorescence allows tracking of differentiating myoblasts in vitro and by intravital imaging in vivo. Lastly, we monitored by live imaging the cell division dynamics of differentiating myoblasts in vitro and showed that a fraction of the MYOGENIN+ population can undergo one round of cell division, albeit at a much lower frequency than MYOGENIN- myoblasts. Conclusions: We expect that this reporter mouse will be a valuable resource for researchers investigating skeletal muscle biology in developmental and adult contexts.

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Section: Discussionmentioning

confidence: 99%

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Benavente-Diaz

Comai

Girolamo

et al. 2020

Preprint

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“…After several hours of in vivo imaging of regenerating muscle to monitor the initial response to muscle injury, cell migration, cell division, and cell fusion of MuSCs using two-photon microscopy or standard confocal microscopy. Konagaya et al (2020) utilized an intravital imaging system for measuring migration rate, extracellular signal-regulated kinase (ERK) activity, and cell cycle of MuSCs within mouse TA muscle. Jacobsen et al injected adult mice with fluorescent dextran and performed intravital imaging to assess in vivo microvascular perfusion of the gluteus maximus (GM) muscle.…”

Section: A Closer Look Into Musc Projections Fluorescent Intravital I...mentioning

confidence: 99%

Imaging analysis for muscle stem cells and regeneration

Karthikeyan,

Asakura

2024

Front. Cell Dev. Biol.

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Composed of a diverse variety of cells, the skeletal muscle is one of the body’s tissues with the remarkable ability to regenerate after injury. One of the key players in the regeneration process is the muscle satellite cell (MuSC), a stem cell population for skeletal muscle, as it is the source of new myofibers. Maintaining MuSC quiescence during homeostasis involves complex interactions between MuSCs and other cells in their corresponding niche in adult skeletal muscle. After the injury, MuSCs are activated to enter the cell cycle for cell proliferation and differentiate into myotubes, followed by mature myofibers to regenerate muscle. Despite decades of research, the exact mechanisms underlying MuSC maintenance and activation remain elusive. Traditional methods of analyzing MuSCs, including cell cultures, animal models, and gene expression analyses, provide some insight into MuSC biology but lack the ability to replicate the 3-dimensional (3-D) in vivo muscle environment and capture dynamic processes comprehensively. Recent advancements in imaging technology, including confocal, intra-vital, and multi-photon microscopies, provide promising avenues for dynamic MuSC morphology and behavior to be observed and characterized. This chapter aims to review 3-D and live-imaging methods that have contributed to uncovering insights into MuSC behavior, morphology changes, interactions within the muscle niche, and internal signaling pathways during the quiescence to activation (Q-A) transition. Integrating advanced imaging modalities and computational tools provides a new avenue for studying complex biological processes in skeletal muscle regeneration and muscle degenerative diseases such as sarcopenia and Duchenne muscular dystrophy (DMD).

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Intravital imaging reveals cell cycle-dependent satellite cell migration during muscle regeneration

Cited by 2 publications

References 72 publications

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Imaging analysis for muscle stem cells and regeneration

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