Intravital mesoscopic fluorescence molecular tomography allows non-invasive in vivo monitoring and quantification of breast cancer growth dynamics

Ozturk, Mehmet S.; Montero, Marta G.; Wang, Ling; Chaible, Lucas Martins; Jechlinger, Martin; Prevedel, Robert

doi:10.1038/s42003-021-02063-8

Cited by 7 publications

(3 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Skin samples included the epidermis, dermis, and adipose tissue. The measured attenuation length was up to 3.5 mm, which is 7 times higher than the experimental values obtained by S. Golovynskyi et al [25] and M. Ozturk et al [34]. Here, the attenuation length values for skin can also depend significantly on the age of the animals, sample storage conditions, and measurement approach used.…”

Section: Introductioncontrasting

confidence: 49%

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Galiakhmetova

Dremin

Koviarov

et al. 2023

IEEE J. Select. Topics Quantum Electron.

View full text Add to dashboard Cite

We present a prototype and verification of a multichannel laser system applicable to optogenetic research. In vivo photostimulation of neural cells expressing photoconvertible phytochromes or opsins requires enough light irradiation delivery to the brain that cannot be supported by continues-wave (CW) light sources. The use of ultra-short pulsed (USP) lasers operating in the second near-infrared region (II-NIR) and allowing nonlinear activation and deactivation of the photoactuators is a promising method that allows to increase the penetration depth and provide spatio-temporal localisation of radiation in tissues. This study aimed to investigate the efficiency of USP light propagation in the skin, skull, and brain of the mouse head, as well as to compare it with the corresponding CW radiation propagation in the 750-830 nm and 1086-1183 nm wavelength ranges. The experimental results and computer modelling demonstrate that about 10-12% of the initial laser radiation can reach the brain tissues. These results prove that under certain conditions, the USP laser radiation can reach a penetration depth with required power that will be sufficient for non-linear activation of opsins/phytochromes in the brain of living animals.

show abstract

Section: Introductioncontrasting

confidence: 49%

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Galiakhmetova

Dremin

Koviarov

et al. 2023

IEEE J. Select. Topics Quantum Electron.

View full text Add to dashboard Cite

show abstract

“…However, the future of imaging must include approaches to mitigate the movement of even more complex specimens. Intravital imaging 198 , 199 is exceptionally powerful for imaging within living vertebrates such as mice or rats. By opening a window to the brain, kidneys, or other organs, one can visualize dynamic processes live within the animal 200 .…”

Section: Introductionmentioning

confidence: 99%

Imagining the future of optical microscopy: everything, everywhere, all at once

Balasubramanian,

Hobson,

Chew

et al. 2023

Commun Biol

View full text Add to dashboard Cite

The optical microscope has revolutionized biology since at least the 17th Century. Since then, it has progressed from a largely observational tool to a powerful bioanalytical platform. However, realizing its full potential to study live specimens is hindered by a daunting array of technical challenges. Here, we delve into the current state of live imaging to explore the barriers that must be overcome and the possibilities that lie ahead. We venture to envision a future where we can visualize and study everything, everywhere, all at once – from the intricate inner workings of a single cell to the dynamic interplay across entire organisms, and a world where scientists could access the necessary microscopy technologies anywhere.

show abstract

“…As a three-dimensional (3D) molecular imaging modality, FMT demonstrates its distinct performance and wide applications in oncology studies, due to its advantages of low cost, high throughput, high specificity, and high sensitivity (5)(6)(7)(8). For example, we previously developed a small animal multimodality imaging and radiotherapy platform (SAMMI-RT), by integrating FMT, x-ray computed tomography (CT) and radiation treatment (9).…”

Section: Introductionmentioning

confidence: 99%

In vivo active-targeting fluorescence molecular imaging with adaptive background fluorescence subtraction

et al. 2023

View full text Add to dashboard Cite

Using active tumor-targeting nanoparticles, fluorescence imaging can provide highly sensitive and specific tumor detection, and precisely guide radiation in translational radiotherapy study. However, the inevitable presence of non-specific nanoparticle uptake throughout the body can result in high levels of heterogeneous background fluorescence, which limits the detection sensitivity of fluorescence imaging and further complicates the early detection of small cancers. In this study, background fluorescence emanating from the baseline fluorophores was estimated from the distribution of excitation light transmitting through tissues, by using linear mean square error estimation. An adaptive masked-based background subtraction strategy was then implemented to selectively refine the background fluorescence subtraction. First, an in vivo experiment was performed on a mouse intratumorally injected with passively targeted fluorescent nanoparticles, to validate the reliability and robustness of the proposed method in a stringent situation wherein the target fluorescence was overlapped with the strong background. Then, we conducted in vivo studies on 10 mice which were inoculated with orthotopic breast tumors and intravenously injected with actively targeted fluorescent nanoparticles. Results demonstrated that active targeting combined with the proposed background subtraction method synergistically increased the accuracy of fluorescence molecular imaging, affording sensitive tumor detection.

show abstract

Intravital mesoscopic fluorescence molecular tomography allows non-invasive in vivo monitoring and quantification of breast cancer growth dynamics

Cited by 7 publications

References 61 publications

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Imagining the future of optical microscopy: everything, everywhere, all at once

In vivo active-targeting fluorescence molecular imaging with adaptive background fluorescence subtraction

Contact Info

Product

Resources

About