Intravital microscopy reveals a novel mechanism of nanoparticles excretion in kidney

Naumenko, Victor; Никитин, А. А.; Kapitanova, Ksenia; Melnikov, Pavel; Vodopyanov, Stepan S.; Garanina, Anastasiia S.; Valikhov, Marat P.; Ilyasov, Artem; Vishnevskiy, Daniil A.; Markov, A. S.; Golyshev, Sergey A.; Жуков, Д. Г.; Alieva, Irina B.; Abakumov, Maxim; Chekhonin, V. P.; Majouga, Alexander G.

doi:10.1016/j.jconrel.2019.06.026

Cited by 45 publications

(30 citation statements)

References 69 publications

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“…Intravital imaging recorded NP transport from peritubular capillaries to the basal compartment of tubular cells, and subsequent excretion to the lumen within 60 min after systemic administration. The results suggest that translocation through the peritubular endothelium to tubular epithelial cells is an alternative mechanism of renal clearance, enabling excretion of NPs above the glomerular cut-off size [48] . The specific uptake of large diameter (350–400 nm) polymer-based mesoscale nanoparticles (MNPs) that targeted the renal proximal tubular epithelial cells has also been observed by IVM [49] .…”

Section: Intravital Imaging To Answer Fundamental Questions About Nanmentioning

confidence: 90%

“…), Tumour endothelial cells (CD31)-Neutrophils (Ly6G) [53] SiO2 nanoparticles Inorganic Pacific blue Spherical 70 PEG n/a Macrophages: within minutes Scavenger endothelial cells: with a time scale of hours in transgenic zebrafish embryos( fli1a:eGFP/mpeg1:mCherry ) [54] Amorphous silica nanoparticles Inorganic Red (569 / 585) Spherical 50 n/a Negative 2 min, 1 h after instillation, alveolar epithelial cells [47] Fe 3 O 4 nanoparticles Inorganic Cy5 Cube, Cluster 140 PEG Negative Within 60 min (i.v. ), Renal tubular epithelial cells [48] PLGA based nanoparticles Organic Cy5 Spherical 347.6 ± 21.0 PEG −19.0 ± 0.3 72 h after injection (i.v. ), Renal tubular epithelial cells [49] Ferumoxytol modified nanoparticles Inorganic FITC Spherical 25.33 33 ± ± 1.70 DEVD peptide conjugated, PEG −3.69 ± −1.29 Day1-Day 6 Labelling mouse mesenchymal stem cells and pig mesenchymal stem cells in vitro [50] Peptide and aptamer dual-functionalized nanoparticles Organic DiD, DiR n/a 106~122 K237 peptide, Ep23 aptamer modified −25.4~−31.4 Within 30 min, Circulating tumour cells (CTC) [52] Cowpea mosaic virus (CPMV) nanoparticles Organic Alexa Fluor 647 Spherical 30 ...…”

Section: Intravital Imaging To Answer Fundamental Questions About Nanmentioning

confidence: 99%

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Nanoparticle delivery in vivo: A fresh look from intravital imaging

2020

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Nanomedicine has proven promising in preclinical studies. However, only few formulations have been successfully translated to clinical use. A thorough understanding of how nanoparticles interact with cells in vivo is essential to accelerate the clinical translation of nanomedicine. Intravital imaging is a crucial tool to reveal the mechanisms of nanoparticle transport in vivo , allowing for the development of new strategies for nanomaterial design. Here, we first review the most recent progress in using intravital imaging to answer fundamental questions about nanoparticle delivery in vivo . We then elaborate on how nanoparticles interact with different cell types and how such interactions determine the fate of nanoparticles in vivo . Lastly, we discuss ways in which the use of intravital imaging can be expanded in the future to facilitate the clinical translation of nanomedicine.

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Section: Intravital Imaging To Answer Fundamental Questions About Nanmentioning

confidence: 90%

Section: Intravital Imaging To Answer Fundamental Questions About Nanmentioning

confidence: 99%

Nanoparticle delivery in vivo: A fresh look from intravital imaging

2020

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“…IO-cubes and IO-clusters were synthetized and functionalized as previously described [64,65]. The physicochemical properties of the IO-cubes and IO-clusters investigated in this study are summarized in Figs.…”

Section: Characterization Of the Nanoparticlesmentioning

confidence: 99%

Progressive lysosomal membrane permeabilization induced by iron oxide nanoparticles drives hepatic cell autophagy and apoptosis

et al. 2020

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Iron oxide nanoparticles (IONs) are frequently used in various biomedical applications, in particular as magnetic resonance imaging contrast agents in liver imaging. Indeed, number of IONs have been withdrawn due to their poor clinical performance. Yet comprehensive understanding of their interactions with hepatocytes remains relatively limited. Here we investigated how iron oxide nanocubes (IO-cubes) and clusters of nanocubes (IO-clusters) affect distinct human hepatic cell lines. The viability of HepG2, Huh7 and Alexander cells was concentration-dependently decreased after exposure to either IO-cubes or IO-clusters. We found similar cytotoxicity levels in three cell lines triggered by both nanoparticle formulations. Our data indicate that different expression levels of Bcl-2 predispose cell death signaling mediated by nanoparticles. Both nanoparticles induced rather apoptosis than autophagy in HepG2. Contrary, IO-cubes and IO-clusters trigger distinct cell death signaling events in Alexander and Huh7 cells. Our data clarifies the mechanism by which cubic nanoparticles induce autophagic flux and the mechanism of subsequent toxicity. These findings imply that the cytotoxicity of ION-based contrast agents should be carefully considered, particularly in patients with liver diseases.

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“…In particular, reports show that 10nm diameter silver nanoparticles are enriched in several organs (e.g., liver, spleen, kidney, testis, thymus, heart, lung and brain, etc.). Larger nanoparticles are specific to blood, liver, and spleen, implicating that 2-6 nm diameter silver nanoparticles are more evenly distributed and less enriched in tissues [32][33][34] . In vivo, the nanoparticles continuously induce inflammation and other toxic reactions.…”

Section: Introductionmentioning

confidence: 99%

“…The renal filtration threshold of nanoparticle size is generally 5.5nm. However, there is no report on whether larger nanoparticles are metabolized by the kidney, which may be related to its rigidity [33,[35][36][37] .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of ultra-small GSH-AgNCs with excellent eschar penetration and antibacterial properties for the healing of burn infection wounds

Yang

Liu

et al. 2021

Preprint

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There is evidence of bioburden as a barrier to chronic burn wound healing. Compared to traditional therapy, nanotechnology has availed a revolutionary approach to therapeutic and diagnostic applications in burns. In this article, we developed the glutathione-protected Ag nanoclusters (GSH-AgNCs) to manage burn wound infection. Owing to the specific structure, the GSH-AgNCs emitted strong red fluorescence under UV excitation, quantified via both in vivo and in vitro techniques. The GSH-AgNCs showed a significant inhibition potential on the proliferation of Staphylococcus aureus ( S. aureus ), Escherichia coli ( E. coli ) , Pseudomonas aeruginosa ( P. aeruginosa ) , and methicillin-resistant staphylococcus aureus (MRSA), hiding under the eschar. Of note, with 2-6nm particle size, GSH-AgNCs are effected in renal excretion, advocating for their biomedical and pharmacological applications.

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Intravital microscopy reveals a novel mechanism of nanoparticles excretion in kidney

Cited by 45 publications

References 69 publications

Nanoparticle delivery in vivo: A fresh look from intravital imaging

Nanoparticle delivery in vivo: A fresh look from intravital imaging

Progressive lysosomal membrane permeabilization induced by iron oxide nanoparticles drives hepatic cell autophagy and apoptosis

Fabrication of ultra-small GSH-AgNCs with excellent eschar penetration and antibacterial properties for the healing of burn infection wounds

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