Intravitreal Saline Injection Ameliorates Laser-Induced Retinal Damage in Rats

Belokopytov, Mark; Shulman, Shiri; Dubinsky, Galina; Belkin, Michael; Rosner, Mordechai

doi:10.1097/iae.0b013e318234942f

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…In the current study, the wavelength, power and exposure time of the laser were precisely controlled to avoid breaking Bruch’s membrane. Simillar laser lesions (spot size 200 μm) had been produced in rats in other studies 33 34 . In our experiments, twenty laser spots were generated on each retina in such a manner that every laser spot is at least two-spot diameters (200 μm) away from others.…”

Section: Discussionmentioning

confidence: 99%

Exosomes derived from MSCs ameliorate retinal laser injury partially by inhibition of MCP-1

Shao

et al. 2016

Sci Rep

169

125

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Although accumulated evidence supports the notion that mesenchymal stem cells (MSCs) act in a paracrine manner, the mechanisms are still not fully understood. Recently, MSC-derived exosomes (MSC-Exos), a type of microvesicle released from MSCs, were thought to carry functional proteins and RNAs to recipient cells and play therapeutic roles. In the present study, we intravitreally injected MSCs derived from either mouse adipose tissue or human umbilical cord, and their exosomes to observe and compare their functions in a mouse model of laser-induced retinal injury. We found that both MSCs and their exosomes reduced damage, inhibited apoptosis, and suppressed inflammatory responses to obtain better visual function to nearly the same extent in vivo. Obvious down-regulation of monocyte chemotactic protein (MCP)-1 in the retina was found after MSC-Exos injection. In vitro, MSC-Exos also down-regulated MCP-1 mRNA expression in primarily cultured retinal cells after thermal injury. It was further demonstrated that intravitreal injection of an MCP-1-neutralizing antibody promoted the recovery of retinal laser injury, whereas the therapeutic effect of exosomes was abolished when MSC-Exos and MCP-1 were administrated simultaneously. Collectively, these results suggest that MSC-Exos ameliorate laser-induced retinal injury partially through down-regulation of MCP-1.

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Section: Discussionmentioning

confidence: 99%