InTray GC Medium Versus Modified Thayer-Martin Agar Plates for Diagnosis of Gonorrhea from Endocervical Specimens

Beverly, A.; Bailey-Griffin, Jill R.; Schwebke, Jane R.

doi:10.1128/jcm.38.10.3825-3826.2000

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…This study clearly demonstrates that a nutritive system such as the InTray GC would be optimal to transport viable N. gonorrhoeae to the laboratory. These results support a previous study that demonstrated equivalent recovery of N. gonorrhoeae from InTray GC and MTM plates inoculated with endocervical specimens from women presenting at a sexually transmitted disease clinic (Beverly et al, 2000). Acceptability of a non-nutritive transport system was limited to Amies medium inoculated with either the Copan Eswab or BD CultureSwab MaxV.…”

supporting

confidence: 91%

Recovery of Neisseria gonorrhoeae from 4 commercially available transport systems

Papp

Henning

Khubbar

et al. 2016

Diagnostic Microbiology and Infectious Disease

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supporting

confidence: 91%

Recovery of Neisseria gonorrhoeae from 4 commercially available transport systems

Papp

Henning

Khubbar

et al. 2016

Diagnostic Microbiology and Infectious Disease

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“…Further, N. gonorrhoeae isolates were confirmed by Real Time PCR assay. For preservation, gonococcal strains were subcultured onto plain MTM agar plates, incubated in similar conditions [38], harvested after a 24 h. incubation and preserved in duplicates in skimmed milk with 10% glycerol. The duplicates were stored at −20 • C freezer until the time for analysis.…”

Section: Clinical Isolatesmentioning

confidence: 99%

“…The overall prevalence of PPNG was 73.7% (95% CI: 65.7, 80.8%), with 56.7% and 43.3% observed in women and men, respectively. The overall prevalence of QRNG was 87.8% and those having high-level resistance [HLR], low-level resistance [LLR] and susceptible (NQR) were 52.4% (86), 23.2% (38), 24.4% (40), respectively. Prevalence of TRNG, non-TRNG and tetracycline-susceptible were 89.3% (125), 9.3% (13) and 1.4% (2), respectively.…”

Section: Prevalence Of Antimicrobial and Multi-drug Resistancementioning

confidence: 99%

“…Thirteen groups had LLR, to quinolone and the remaining fourteen had non-quinolone resistance (NQR-series) with MDR and XDR, respectively. The Q[H]-series that consisted of the predominant group accounted for 52.3% (83) of all isolates, followed by the Q[L]-series 23.2% (38) and NQR-series 24.4% (40) (Figure 1).…”

Section: Prevalence Of Antimicrobial and Multi-drug Resistancementioning

confidence: 99%

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Antimicrobial Susceptibility Testing Patterns of Neisseria gonorrhoeae from Patients Attending Sexually Transmitted Infections Clinics in Six Regions in Tanzania

et al. 2022

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Antimicrobial resistance (AMR) is global health threat that is on the increase, and it has been adversely affecting the proper management of sexually transmitted infections (STI). Data on antimicrobial susceptibility testing patterns of N. gonorrhoeae are limited in local settings. We determined in vitro antimicrobial susceptibility and phenotypic profiles of N. gonorrhoeae isolated from STI/Outpatient Department (OPD) clinics. Minimum Inhibitory Concentrations (MIC) (µg/mL) were determined using E-Test and agar dilution methods for previously and currently recommended antimicrobial agents. A total of 164 N. gonorrhoeae isolates from urethral discharge and endocervical swabs were tested. The prevalence of resistant N. gonorrhoeae to tetracycline, norfloxacin, penicillin and ciprofloxacin were 98.6%, 82.2%, 84.3% and 75.6%, respectively. None of the isolates was resistant to kanamycin. Penicillinase producing N. gonorrhoeae (PPNG) was found to be 73.7%, with 56.7% and 43.3% observed among isolates from women and men, respectively. Tetracycline resistant-N. gonorrhoeae (TRNG) was found to be 34.0%, and QRNG with HLR to ciprofloxacin was 79.9%. The overall MDR-NG was 79.9%, and XDR-NG was 3.6%. MIC50 and MIC90 were 4.0 and 8.0 and 2.0 and 4.0 µg/mL for ciprofloxacin and norfloxacin, respectively. Dendrograms showed that 44 phenotypic groups are associated with a high rate of AMR among high MDR-NG and moderate XDR-NG isolates. The predominant groups of quinolone-resistant N. gonorrhoeae (QRNG)+PPNG (34.7%) and QRNG+PPNG+TRNG (32.9%) were observed among the isolates having HLR to ciprofloxacin. We reported a high prevalence of AMR (>90%) to previously recommended antimicrobials used for the treatment of gonorrhoea. Multidrug resistant N. gonorrhoeae (MDR-NG) was highly reported, and extensively drug resistant (XDR-NG) has gradually increased to the currently recommended cephalosporins including ceftriaxone and cefixime. Heterogeneous groups of QRNG+PPNG+ and QRNG+PPNG+TRNG were highly resistant to penicillin, tetracycline, ciprofloxacin and norfloxacin. A surveillance program is imperative in the country to curb the spread of AMR.

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“…Prior studies have examined the difference in viability between different commercially available transport systems and generally found that the InTray GC1, a CO 2 generating packaging medium manufactured by BioMed Diagnostics, had a high rate of recovery after extended transport times [7]. Additionally, InTray GC plates have a shelf life of approximately one year, so are well-suited for use in settings where standard chocolate agar plates may not be widely available or viable after storage [8]. In an effort to build upon these previous studies, we assessed bacterial recovery of clinical isolates of N. gonorrhoeae in transport conditions in the InTray GC 72 hours after an initial period of incubation.…”

Section: Introductionmentioning

confidence: 99%

72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray® GC method

et al. 2022

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Recovery of Neisseria gonorrhoeae isolates exposed to a range of transport times and temperatures was quantitatively assessed for two transport devices, BioMed Diagnostics’ InTray GC® and Copan Diagnostics’ Liquid Amies Elution Swab (ESwab®) Collection and Transport System. Respective devices were inoculated with N. gonorrhoeae, exposed to simulated transport conditions and spread-plated from serial dilutions in duplicates onto chocolate agar in order to count CFU (colony-forming units) in the range of 25–250. Baseline CFU/mL averages of time-zero transport for each device was compared to either 24 hour (Eswab) or 72 hour (InTray GC) CFU/mL average to assess recovery of six clinical isolates of N. gonorrhoeae, and differences showing no greater than a 3 log10 (± 10%) decline between comparative time points qualified as acceptable. Our findings suggest that the InTray GC system has the potential to transport clinical isolates for ≤72 hours with acceptable N. gonorrhoeae recovery.

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InTray GC Medium Versus Modified Thayer-Martin Agar Plates for Diagnosis of Gonorrhea from Endocervical Specimens

Abstract: A new self-contained medium system for the cultivation ofNeisseria gonorrhoeae was compared to modified Thayer-Martin medium for the diagnosis of gonorrhea from endocervical specimens. There was no difference in the ability of the two methods to support the growth of N. gonorrhoeae.

Cited by 10 publications

References 8 publications

Recovery of Neisseria gonorrhoeae from 4 commercially available transport systems

Recovery of Neisseria gonorrhoeae from 4 commercially available transport systems

Antimicrobial Susceptibility Testing Patterns of Neisseria gonorrhoeae from Patients Attending Sexually Transmitted Infections Clinics in Six Regions in Tanzania

72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray® GC method

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