2015
DOI: 10.1073/pnas.1424704112
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Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes

Abstract: Biocontainment may be required in a wide variety of situations such as work with pathogens, field release applications of engineered organisms, and protection of intellectual properties. Here, we describe the control of growth of the brewer’s yeast, Saccharomyces cerevisiae, using both transcriptional and recombinational “safeguard” control of essential gene function. Practical biocontainment strategies dependent on the presence of small molecules require them to be active at very low concentrations, rendering… Show more

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Cited by 65 publications
(76 citation statements)
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“…In addition, comparison of the three best SG strains identified here with the previously published ones (20) shows significantly lower background growth on YPD medium compared with YPGAL ( Fig. 2D), indicating that this screen enabled selection of better-performing SG strains.…”
Section: Resultssupporting
confidence: 59%
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“…In addition, comparison of the three best SG strains identified here with the previously published ones (20) shows significantly lower background growth on YPD medium compared with YPGAL ( Fig. 2D), indicating that this screen enabled selection of better-performing SG strains.…”
Section: Resultssupporting
confidence: 59%
“…2C). Similar to the previously described histone H3/H4 SG strain (20), all SG strains show a slight increase in doubling time compared with the shuffle strain, which we attribute to the influence of induction of endogenous GAL genes by estradiol (28). Of the 13 strains, we chose one SG strain for each essential gene to follow up with an escape (reversion) rate experiment as a final test for optimality and identification of the promoters.…”
Section: Resultsmentioning
confidence: 83%
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“…4 and 5) and insertions (27) of DNA. Thus, this system may have value for complex manipulations, including synthesis of designer chromosomes from scratch, as described by several groups (28)(29)(30). The ability of the IntXO protein to recognize and recombine PSL sites in vitro may also be useful for future work in the synthetic biology field.…”
Section: Discussionmentioning
confidence: 99%