Intrinsic Factor

Ellenbogen, Leon; Highley, Derek R.

doi:10.1016/s0083-6729(08)60237-x

Cited by 9 publications

(6 citation statements)

References 226 publications

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“…The trace amount of protein with 12.7 S may be polymers of hog IF. Moreover, the forms of hog IF, either in B12 uncomplexed or in B12 complexed preparation, give different values of the molecular weight by interconver -sions of monomer and polymer (7). Based on the finding, the purified IF in the present study is likely to be a monomer.…”

Section: Discussionmentioning

confidence: 63%

“…However, some investi gators have reported two B12 binding sub stances, IF active and inactive, in the pre paration of purified hog IF (7,12,13). This discrepancy may come from the differences in the chromatography systems used.…”

Section: Discussionmentioning

confidence: 99%

“…The procedures were devised from the combinations of chemical fraction a tion, (3)(4)(5), enzyme treatment, (6, 7), prepara tive electrophoresis (8,9), preparative ultra -centrifugation (10,11), and ion exchange column chromatography (7,(11)(12)(13).…”

mentioning

confidence: 99%

“…In 1961, Holdsworth isolated the highly purified homogeneous fraction II active at 0.5 mg by cellulose column chromatography and analyzed its properties in detail (12). Recently, Ellenbogen et al, reported more potent hog IF by cellulose column chromatography com bined with the enzymatic digestion method (7), and also studied the characteristics of hog IF in detail (17).…”

mentioning

confidence: 99%

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Studies on Hog Intrinsic Factor

Inada

1970

J. Vitaminol

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Studies on Hog Intrinsic Factor

Inada

1970

J. Vitaminol

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“…It is generally accepted that IF is a glycoprotein secreted from the stomach and participates in the mechanism of uptake of vitamin B12 from the small intestine in animals (1)(2)(3)(4)(5).…”

mentioning

confidence: 99%

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Sasaki

1971

J. Vitaminol

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The B12-binding principles isolated from cellular components of Lacotobacillus leichmannii ATCC 7830 were studied for the activity to augment vitamin B12 uptake by intestinal mucosa homogenates (IMH) of the guinea pig, hog, rat, hamster, and man. It was found that the uptake of B12 by the IMH of these species was markedly augmented by these principles. The degrees of augmenta tion were much greater than that effected by the homologous intrinsic factor (IF). The IF-augmented uptake of B12 by guinea pig IMH was reduced when calcium ion was deleted from the incubation medium, while the deletion of calcium ion had no effect on the uptake augmented by the B12-binding principle. Boiling of IMH for 10min completely abolished the IF-augmented uptake of B12 but the effect of the B12-binding principle was not affected at all. These results indicate that difference exists between IF and the B12-binding principle in the mode of attachment to the adsorptive surface of the small intestine. The B12 uptake by everted sacs prepared from the distal portion of guinea pig ileum was slightly augmented by guinea pig IF but not by the B12-binding princinciple. However, the mucous materials liberated into the medium from the everted sacs during incubation took up large amounts of B12 in the presence of the B12 binding principle as well as IF.

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Malnutrition

Lancker¹

1976

Molecular and Cellular Mechanisms in Disease

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Intrinsic Factor

Cited by 9 publications

References 226 publications

Studies on Hog Intrinsic Factor

Studies on Hog Intrinsic Factor

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Malnutrition

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