Intrinsic Flexibility of Nucleic Acid Chaperone Proteins from Pathogenic RNA Viruses

Ivanyi‐Nagy, Roland; Makowska, Zuzanna; Darlix, Jean‐Luc

doi:10.1002/9781118183373.ch10

Cited by 1 publication

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“…But at the same time these NC-based oligomeric structures are accessible to small ODNs and to the RT enzyme which can gain access to the RNA and perform cDNA synthesis with high efficiency. Therefore such NC-based oligomers are either porous or else highly dynamic, the latter being probably the case due to the rapid on and off binding kinetics of NC to the nucleic acid (Cruceanu et al, 2006a) together with different modes of interactions driven by the ZFs and/or the disordered basic domains (reviewed in Darlix et al, 2011;Ivanyi-Nagy et al, 2012). Taken together, these findings favor the notion that NC-RNA ribonucleoprotein complexes can be regarded as fuzzy flexible molecular assemblages where multiple different interactions between the viral NC and the NA (Fig.…”

Section: Viral Nc-rna Rnps Fuzziness and Functionsmentioning

confidence: 99%

Retrospective on the all-in-one retroviral nucleocapsid protein

et al. 2014

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This review aims at briefly presenting a retrospect on the retroviral nucleocapsid protein (NC), from an unspecific nucleic acid binding protein (NABP) to an all-in-one viral protein with multiple key functions in the early and late phases of the retrovirus replication cycle, notably reverse transcription of the genomic RNA and viral DNA integration into the host genome, and selection of the genomic RNA together with the initial steps of virus morphogenesis. In this context we will discuss the notion that NC protein has a flexible conformation and is thus a member of the growing family of intrinsically disordered proteins (IDPs) where disorder may account, at least in part, for its function as a nucleic acid (NA) chaperone and possibly as a protein chaperone vis-à-vis the viral DNA polymerase during reverse transcription. Lastly, we will briefly review the development of new anti-retroviral/AIDS compounds targeting HIV-1 NC because it represents an ideal target due to its multiple roles in the early and late phases of virus replication and its high degree of conservation.

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Section: Viral Nc-rna Rnps Fuzziness and Functionsmentioning

confidence: 99%