Intrinsic GUS-like activities in seed plants

Hu, Chao; Chee, Paula P.; Chesney, Robert H.; Zhou, Junhui; Miller, Paul D.; O'brien, W. T.

doi:10.1007/bf00232123

Cited by 101 publications

(45 citation statements)

References 7 publications

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“…Subsequent handling of tissue sections (relaxation, binding to microscope slides, solubilization of paraffin with xylene) was performed as described by Schmelzer et al (1 989). Using these methods, endogenous GUS activity, as described in certain floral organs (Plegt and Bino, 1989;Hu et al, 1990), was not observed in any organs examined from untransformed tobacco plants or tobacco plants transgenic for a promoterless GUS gene.…”

Section: Histochemical Localization Of Gus Activitymentioning

confidence: 98%

A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco.

et al. 1991

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The 4CL-1 gene is one of two highly homologous parsley genes encoding 4-coumarate:coenzyme A ligase, a key enzyme of general phenylpropanoid metabolism. Expression of these genes is essential for the biosynthesis of both defense-related and developmentally required phenylpropanoid derivatives. We examined the developmental regulation of the 4CL-1 promoter by analyzing the expression of 4CL-1-8-glucuronidase fusions in transgenic tobacco plants. A 597-base pair 4CL-1 promoter fragment specified histochemically detectable expression in a complex array of vegetative and floral tissues and cell types. The activity of a series of 5' deleted promoter fragments was analyzed in parsley protoplasts and transgenic tobacco plants. Deletions past -210 base pairs led to a drastic decline in 8-glucuronidase activity in protoplasts and loss of tissue-specific expression in transgenic tobacco. These results were put into the context of potential protein-DNA interactions by in vivo footprint analysis of the 4CL-1 promoter in parsley cells. Loss of promoter activity in parsley protoplasts and transgenic tobacco was correlated with the deletion or disruption of the dista1 portion of a large (100-base pair) footprinted region within the first 200 base pairs of the 4CL-1 promoter.

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Section: Histochemical Localization Of Gus Activitymentioning

confidence: 98%

A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco.

et al. 1991

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“…Intrinsic GUS activity has been (MCAT) gave no significant changes in the composition and reported in B. napus leaves (Hodal et al 1992) and in mature quantity of storage lipids. However, by use of the seed-specific embryos of B. rapa and B. oleracea (Hu et al 1990).…”

Section: Assessment Of Transgenic Plantsmentioning

confidence: 99%

Genetic transformation of Brassica

Poulsen

1996

Plant Breeding

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Tissue culture regeneration of plants from Brassica species can be achieved from various explant types. Their performance, however, varies with the genotype and age of the explant and is sensitive to medium supplements and Agrobacterium cocultivation. Most Brassica transformations were carried out using Agrobacterium and it was established that nopaline and agropine strains of .4. tumefaciens and A. rhizogenes, respectively, are the most efficient. Application of virulence gene induction is ambiguous. Binary vectors are mostly apphed in spite of indications that cointegrate vectors are more efficient. Cotransformation is successful with two different bacteria or with one bacterium carrying both plasmids, however, it has proved problematic to integrate the two transgenes into separate loci. Transformations with wild type rol genes develop hairy roots, from which transgenic plants can be regenerated. Vectorless transformation is feasible with microprojectile bombardment of microspore derived embryos being the most promising.Antibiotic markers providing resistance to kanamycin, hygromycin, and spectinomycin are applicable as selectable markers, while chloramphenicol is not suitable. Among herbicides, phosphinotricin gives good results while chlorsulfuron is unsuitable for in vitro selection. Major therapeutic antibiotics being successfully applied are carbenicillin, cefotaxime, amoxycillin, and ticarcillin. Standard methods for evaluating transgenic plants also have been applied to Brassica species. Some breeding objectives obtained through genetic transformation are presented.

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“…Exposure of humans to the GUS protein is common, because GUS is present in intestinal epithelial cells, intestinal microflora bacteria, and numerous foods, and no harmful effects have been reported (Gilissen et al, 1998). GUS activity has been detected in over 50 plant species (Hu et al, 1990). These species include a number of human food sources, including potato, apple, almond, rye, rhubarb, and sugar beet (Schulz and Weissenbock, 1987;Hodal et al, 1992;Wozniak and Owens, 1994).…”

Section: Characterization and History Of Safe Consumption Of Cry2ab2 mentioning

confidence: 99%

Organisms with potential to assist in the control of Helicoverpa armigera in Australian cotton production systems

et al. 2016

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Executive SummaryBollgard II cotton event 15985 was developed by Monsanto Company to produce the Cry2Ab2 insect control protein, which provides effective season-long control of key lepidopteran insect pests. This product was produced by re-transformation of Bollgard ® cotton event 531, which produces the Cry1Ac insect-control protein and the NPTII selectable marker protein. Therefore, Bollgard II cotton produces two proteins for effective control of the major lepidopteran insect pests of cotton, including the cotton bollworm, tobacco budworm, pink bollworm, and armyworm. Bollgard II cotton also produces the β-D-glucuronidase (GUS) marker protein. In addition, Bollgard II cotton provides a more effective insect resistance management program compared to single gene products.

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Intrinsic GUS-like activities in seed plants

Cited by 101 publications

References 7 publications

A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco.

A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco.

Genetic transformation of Brassica

Organisms with potential to assist in the control of Helicoverpa armigera in Australian cotton production systems

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