Intrinsic regulation of hemoglobin expression by variable subunit interface strengths

Manning, James M.; Popowicz, Anthony; Padovan, Júlio C.; Chait, Brian T.; Manning, Lois R.

doi:10.1111/j.1742-4658.2011.08437.x

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…Acetylation of ADP-ribosylation factor-like protein 8B (ARL8B) by NatC targets the protein to the lysosomal membrane (Starheim et al 2009 ). Aberrant N-terminal acetylation of hemoglobin can cause clinical diseases related to resulting abnormal oxygenbinding capacity of the holoprotein (Starheim et al 2009 ;Manning et al 2012 ).…”

Section: Protein Acetylationmentioning

confidence: 99%

Post-translational Modifications in the Human Proteome

Lichti

Wildburger

Emmett

et al. 2014

Translational Bioinformatics

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The Chromosome-Centric Human Proteome Project (C-HPP) is a global project aimed to identify at least one protein isoform encoded by the approximately 20, 300 human genes. In addition, protein post-translational modifi cations will be characterized, with the initial goal of detecting phosphorylation, acetylation, and glycosylation sites in each protein. In this chapter, we provide an overview of known post-translational modifi cations, their known biological functions, and present strategies to detect them on both a single protein and proteomic scales. In future proteomic studies, global characterization of post-translation modifi cations, splice variants, and variants caused by single nucleotide polymorphisms (SNPs) will be necessary to fully understand the role of proteins in human biology and disease.

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Section: Protein Acetylationmentioning

confidence: 99%

Post-translational Modifications in the Human Proteome

Lichti

Wildburger

Emmett

et al. 2014

Translational Bioinformatics

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“…During gestation, a sequential activation of genes responsible for the production of globin chains occurs, leading to the formation of different types of embryonic and fetal Hb that differ from each other according to their constitutive chains (66). These newly synthesized chains are capable of binding to heme and iron, forming the Hb molecule.…”

Section: Hemoglobin Role Of Erythropoietin In Erythropoiesis and Thementioning

confidence: 99%

Erythropoiesis in vertebrates from ontogeny to clinical relevance

et al. 2016

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Erythropoiesis is a complex process that starts in the course of embryo formation and it is maintained throughout the life of an organism. During the fetal development, erythropoiesis arises from different body sites and erythroblast maturation occurs in the fetal liver. After birth, erythropoiesis and erythroblast maturation take place exclusively in the bone marrow, generating a lifetime reservoir of red blood cells (RBCs), which are responsible for transporting oxygen through the bloodstream to tissues and organs. Several transcription factors and cytokines, such as GATA-1, GATA-2, FOG-1 and erythropoietin (EPO), constitute an elaborated molecular network that regulates erythropoiesis as they are involved in the differentiation and maturation of RBCs. The profound understanding of erythropoiesis is fundamental to avoid, treat or even soften the effects of erythropoietic clinical disorders and may be useful to improve patients' well-being.

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“…The transition of Hb from embryonic protein isoforms, known as fetal Hb, to adult isoforms is typically assayed by gel electrophoresis (31)(32)(33). Fetal Hb binds oxygen with greater affinity than does adult Hb, which allows the former to compete effectively for oxygen with adult Hb in the placental blood.…”

Section: Significancementioning

confidence: 99%

Microscopy ambient ionization top-down mass spectrometry reveals developmental patterning

Hsu¹,

White

Hayashi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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There is immense cellular and molecular heterogeneity in biological systems. Here, we demonstrate the utility of integrating an inverted light microscope with an ambient ionization source, nanospray electrospray desorption ionization, attached to a highresolution mass spectrometer to characterize the molecular composition of mouse spinal cords. We detected a broad range of molecules, including peptides and proteins, as well as metabolites such as lipids, sugars, and other small molecules, including S-adenosyl methionine and glutathione, through top-down MS. Top-down analysis revealed variation in the expression of Hb, including the transition from fetal to adult Hb and heterogeneity in Hb subunits consistent with the genetic diversity of the mouse models. Similarly, temporal changes to actin-sequestering proteins β-thymosins during development were observed. These results demonstrate that interfacing microscopy with ambient ionization provides the means to perform targeted in situ ambient top-down mass spectral analysis to study the pattern of proteins, lipids, and sugars in biologically heterogeneous samples.

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Intrinsic regulation of hemoglobin expression by variable subunit interface strengths

Cited by 6 publications

References 30 publications

Post-translational Modifications in the Human Proteome

Post-translational Modifications in the Human Proteome

Erythropoiesis in vertebrates from ontogeny to clinical relevance

Microscopy ambient ionization top-down mass spectrometry reveals developmental patterning

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