Intrinsic Wavelength Standard Absorption Bands in Holmium Oxide Solution for UV/visible Molecular Absorption Spectrophotometry

Travis, John C.; Acosta, Joaquín Campos; Andor, G; Bastié, Jean; Blattner, Peter; Chunnilall, Christopher J.; Crosson, Steven C.; Duewer, David L.; Early, E A.; Hengstberger, F.; Kim, Chang-Soon; Liedquist, Leif; Manoocheri, Farshid; Mercader, Flora; Monard, L. A. G.; Nevas, Saulius; Mito, Akihiro; Nilsson, Morgan; Noël, Mario; Rodriguez, Antonio C.; Ruı́z, Arquı́medes; Schirmacher, A.; Smith, Melody V.; Valencia, Guillermo; Tonder, Natasha van; Zwinkels, Joanne C.

doi:10.1063/1.1835331

Cited by 24 publications

(9 citation statements)

References 2 publications

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“…Comparison against the NIST standard spectrum of holmium (III) oxide (4%, w/v) in perchloric acid (10%, w/v) [14] shows that, within the operating range of the spectrometer, the main calibration bands (Bands 9-14) are present in both spectra, confirming the ability of the instrument to measure absorbance to a high degree of wavelength accuracy. The results show that the working range of the beam splitter in this instrument is approximately λ = 350 to 720 nm.…”

Section: Assessing Wavelength Accuracy Against a Reference Standardmentioning

confidence: 73%

“…This solution is the National Institute of Standards and Technology (NIST) recognised standard solution for spectrometer calibration as its absorbance spectrum has fourteen traceable peaks in the wavelength range 240 to 650 nm. The instrument was calibrated using this standard and peak centres observed were compared with their corresponding reference wavelengths (bands 9-14), as stated by NIST [13,14].…”

Section: = =mentioning

confidence: 99%

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An Inexpensive, Pulsed, and Multiple Wavelength Bench-Top Light Source for Biological Spectroscopy

Holman

Skidmore

Yates

2018

Plasma

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Since signal/noise ratios are proportional to the square root of the intensity, high intensity light sources are advantageous for many forms of UV–Vis and IR spectroscopy particularly with very low or high absorbance samples. We report the construction of a low-cost (≈ £6500 GBP, ca. 2016) bench-top spectrometer suitable for biological spectroscopy, which utilizes a hot plasma, generated with a pulsed Nd:YAG laser (λ = 1064 nm). The properties (reliability, intensity, and spectral profiles) of light generated with the plasma in different gaseous media (helium, neon, argon, and krypton) were investigated. Argon provided high intensity broadband light and was the most cost effective. The instrument was compared for spectral accuracy to a commercially available spectrometer (Thermo Scientific, GENESYS 10S) by measurement of the absorbance spectrum of the UV–Vis calibration standard holmium (III) oxide (4%, w/v) in perchloric acid (10%, w/v) and accurately replicated the results of the commercial spectrometer. This economical instrument can record consecutive absorbance spectra (between λ = 380 and 720 nm) for each laser pulse (6 Hz; ~160 ms/pulse), evinced by investigations into lysozyme aggregation in the presence of heparin. This instrument is suitable for use with lasers of a higher pulse power and repetition rates that would induce higher temperature plasmas. Higher temperature plasma sources offer increased signal to noise ratios due to the higher intensity emission generated.

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Section: Assessing Wavelength Accuracy Against a Reference Standardmentioning

confidence: 73%

Section: = =mentioning

confidence: 99%

An Inexpensive, Pulsed, and Multiple Wavelength Bench-Top Light Source for Biological Spectroscopy

Holman

Skidmore

Yates

2018

Plasma

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“…The transition of the holmium oxide glass standard to one that does not need direct involvement of NIST through a calibration service parallels the transition which was described by Travis, et al for the holmium oxide solution [ 5 ]. That paper discusses the bands as intrinsic values with the establishment of traceability through atomic absorption lines of the Hg lamps that were used to establish the certified bands.…”

Section: Traceabilitymentioning

confidence: 79%

Holmium oxide glass wavelength standards

Allen

2007

J. Res. Natl. Inst. Stand. Technol.

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Holmium oxide glass has been used as a wavelength standard for over four decades. These standards have shown insignificant spectral variation from batch to batch and from one manufacturer to another. The National Institute of Standards and Technology (NIST) has certified and recertified holmium oxide glass samples for over four decades. Over this period of time there has been no recorded instance of a spectral shift of the certified bands for any of the samples measured. Moreover, these samples are known to be robust and relatively insensitive to a normal range of temperature and humidity. Based on the extensive experience that NIST has with this material and its long-term stability, NIST will no longer recommend the recertification of these standards. Furthermore, traceability may be established either through the supplier or by the end user without the need for NIST involvement.

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“…Determination of Spectrophotometer Wavelength Offsets. The wavelength calibrations of five Agilent 8453 UV−visible spectrophotometersall of which had been previously calibrated according to manufacturer's instructions and some of which had been used on recent research cruises were assessed using SRM 2034, 38 a National Institute of Standards and Technology (NIST) holmium oxide wavelength standard. Dilute holmium oxide solution exhibits several distinct absorbance peaks between 240 and 650 nm.…”

Section: −mentioning

confidence: 99%

“…First, for a given spectrophotometer, the wavelength offset (Δλ 241.1 ) is determined using SRM 2034 38 ] spec absorbance data collected with this instrument. If the instrument is recalibrated, the wavelength offset must be redetermined.…”

Section: −mentioning

confidence: 99%

Spectrophotometric Determination of Carbonate Ion Concentrations: Elimination of Instrument-Dependent Offsets and Calculation of In Situ Saturation States

Sharp

Byrne

Liu

et al. 2017

Environ. Sci. Technol.

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This work describes an improved algorithm for spectrophotometric determinations of seawater carbonate ion concentrations ([CO]) derived from observations of ultraviolet absorbance spectra in lead-enriched seawater. Quality-control assessments of [CO] data obtained on two NOAA research cruises (2012 and 2016) revealed a substantial intercruise difference in average Δ[CO] (the difference between a sample's [CO] value and the corresponding [CO] value calculated from paired measurements of pH and dissolved inorganic carbon). Follow-up investigation determined that this discordance was due to the use of two different spectrophotometers, even though both had been properly calibrated. Here we present an essential methodological refinement to correct [CO] absorbance data for small but significant instrumental differences. After applying the correction (which, notably, is not necessary for pH determinations from sulfonephthalein dye absorbances) to the shipboard absorbance data, we fit the combined-cruise data set to produce empirically updated parameters for use in processing future (and historical) [CO] absorbance measurements. With the new procedure, the average Δ[CO] offset between the two aforementioned cruises was reduced from 3.7 μmol kg to 0.7 μmol kg, which is well within the standard deviation of the measurements (1.9 μmol kg). We also introduce an empirical model to calculate in situ carbonate ion concentrations from [CO]. We demonstrate that these in situ values can be used to determine calcium carbonate saturation states that are in good agreement with those determined by more laborious and expensive conventional methods.

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Intrinsic Wavelength Standard Absorption Bands in Holmium Oxide Solution for UV/visible Molecular Absorption Spectrophotometry

Cited by 24 publications

References 2 publications

An Inexpensive, Pulsed, and Multiple Wavelength Bench-Top Light Source for Biological Spectroscopy

An Inexpensive, Pulsed, and Multiple Wavelength Bench-Top Light Source for Biological Spectroscopy

Holmium oxide glass wavelength standards

Spectrophotometric Determination of Carbonate Ion Concentrations: Elimination of Instrument-Dependent Offsets and Calculation of In Situ Saturation States

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