Intrinsically disordered proteins and biomineralization

Boskey, Adele L.; Villarreal-Ramírez, Eduardo

doi:10.1016/j.matbio.2016.01.007

Cited by 130 publications

(139 citation statements)

References 158 publications

(185 reference statements)

Supporting

Mentioning

136

Contrasting

Order By: Relevance

“…Previously, Chou et al described that the bone sialoprotein (BSP) and osteocalcin (OCN) expression of MC3T3 cells (osteoblastic cell line) were affected by the size of apatite crystals. Importantly, greater expression of both BSP and OCN were shown on larger crystals . In our study, we demonstrate that both the polymer ratio and concentration affect the degree of mineralization and consequently, an optimal polymer‐calcium complex could be achieved to promote osteogenic differentiation and mineralization.…”

Section: Discussionmentioning

confidence: 60%

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

Wang

Wimpenny

Dey

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

There is a clear clinical need for a bioactive bone graft substitute. Poly(vinyl phosphonic acid‐co‐acrylic acid) (PVPA‐co‐AA) has been identified as a promising candidate for bone regeneration but there is little evidence to show its direct osteogenic effect on progenitor or mature cells. In this study mature osteoblast‐like cells (SaOS‐2) and human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) were cultured with PVPA‐co‐AA polymers with different VPA:AA ratio and at different concentrations in vitro. We are the first to report the direct osteogenic effect of PVPA‐co‐AA polymer on bone cells and, more importantly, this effect was dependent on VPA:AA ratio and concentration. Under the optimized conditions, PVPA‐co‐AA polymer not only has an osteoconductive effect, enhancing SaOS‐2 cell mineralization, but also has an osteoinductive effect to promote hBM‐MSCs’ osteogenic differentiation. Notably, the same PVPA‐co‐AA polymer at different concentrations could lead to differential osteogenic effects on both SaOS‐2 and hBM‐MSCs in vitro. This study furthers knowledge of the PVPA‐co‐AA polymer in osteogenic studies, which is critical when utilizing the PVPA‐co‐AA polymer for the design of novel bioactive polymeric tissue engineering scaffolds for future clinical applications. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 168–179, 2018.

show abstract

Section: Discussionmentioning

confidence: 60%

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

Wang

Wimpenny

Dey

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…[1,3] SMPs are often highly negatively charged acidic proteins, which undergo extensive post-translational modifications, and frequentlyb elong to the group of intrinsically disordered pro-teins (IDPs). [4][5][6][7] Additionally,p roteins that act indirectly create the required conditions for the formation of mineral deposits. For example, certain classes of proteins are responsible for appropriate ion-concentration maintenance, some enzymes are involved in post-translational modifications, whereas other macromolecules mayc hange the conformation of the nucleating proteins or organization of the mineralizing tissue.…”

Section: Introductionmentioning

confidence: 99%

Lattice Shrinkage by Incorporation of Recombinant Starmaker‐Like Protein within Bioinspired Calcium Carbonate Crystals

Różycka

Coronado

Brach

et al. 2019

Chemistry A European J

View full text Add to dashboard Cite

The biological mediation of mineral formation (biomineralization) is realized through diverse organic macromolecules that guide this process in a spatial and temporal manner. Although the role of these molecules in biomineralization is being gradually revealed, the molecular basis of their regulatory function is still poorly understood. In this study, the incorporation and distribution of the model intrinsically disordered starmaker‐like (Stm‐l) protein, which is active in fish otoliths biomineralization, within calcium carbonate crystals, is revealed. Stm‐l promotes crystal nucleation and anisotropic tailoring of crystal morphology. Intracrystalline incorporation of Stm‐l protein unexpectedly results in shrinkage (and not expansion, as commonly described in biomineral and bioinspired crystals) of the crystal lattice volume, which is described herein, for the first time, for bioinspired mineralization. A ring pattern was observed in crystals grown for 48 h; this was composed of a protein‐enriched region flanked by protein‐depleted regions. It can be explained as a result of the Ostwald‐like ripening process and intrinsic properties of Stm‐l, and bears some analogy to the daily growth layers of the otolith.

show abstract

“…Noncollagenous proteins (NCPs) are involved in formation of bones and teeth; a large proportion of these NCPs are intrinsically disordered [61]. Over the last several years, many researchers have focused on understanding the mechanism of these NCPs and IDPs in the control of HA crystal growth.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation regulates the secondary structure and function of dentin phosphoprotein peptides

Villarreal-Ramírez

Eliezer

Garduño‐Juárez³

et al. 2017

Bone

Self Cite

View full text Add to dashboard Cite

Dentin phosphoprotein (DPP) is the most acidic protein in vertebrates and structurally is classified as an intrinsically disordered protein. Functionally, DPP is related with dentin and with bone formation, however the specifics of such association remain unknown. Here, we used atomistic molecular dynamics simulations to screen selected binding domains of DPP onto hydroxyapatite (HA), which is one of its important interacting partners. From these results, we selected a functionally relevant peptide, Ace-SSDSSDSSDSSDSSD-NH2 (named P5) and its phosphorylated form (named P5P), for experimental characterization. SAXS experiments indicated that in solution P5 was disordered, possibly in an extended conformation while P5P displayed more compact globular conformations. Circular dichroism and FTIR confirmed that, either in the presence or absence of Ca2+/HA, P5 adopts a random coil structure, whereas its phosphorylated counterpart, P5P, has a more compact arrangement associated with conformations that display β-sheet and α-helix motifs when bound to HA. In solution, P5 inhibited HA crystal growth, whereas at similar concentrations, P5P stimulated it. These findings suggest that phosphorylation controls the transient formation of secondary and tertiary structure of DPP peptides, and, most likely of DPP itself, which in turn controls HA growth in solution and possibly HA growth in mineralized tissues.

show abstract

Intrinsically disordered proteins and biomineralization

Cited by 130 publications

References 158 publications

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

Lattice Shrinkage by Incorporation of Recombinant Starmaker‐Like Protein within Bioinspired Calcium Carbonate Crystals

Phosphorylation regulates the secondary structure and function of dentin phosphoprotein peptides

Contact Info

Product

Resources

About