Introducing glycophage arrays: Facile production, purification and patterning of glycophages

Lajoie, Jason; Shusta, Eric V.

doi:10.1002/biot.201400591

Cited by 2 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aberrations in glycosylation of proteins have been implicated in several diseases and many array‐based techniques have emerged, including glycol arrays to study glycan–protein interaction . A recent study has made use of glycosphingolipid arrays to identify Aabs in patients with peripheral neuropathy .…”

Section: Application Of Protein Arrays In Ptm Studiesmentioning

confidence: 99%

Protein microarray applications: Autoantibody detection and posttranslational modification

et al. 2016

View full text Add to dashboard Cite

The discovery of DNA microarrays was a major milestone in genomics; however, it could not adequately predict the structure or dynamics of underlying protein entities, which are the ultimate effector molecules in a cell. Protein microarrays allow simultaneous study of thousands of proteins/peptides, and various advancements in array technologies have made this platform suitable for several diagnostic and functional studies. Antibody arrays enable researchers to quantify the abundance of target proteins in biological fluids and assess PTMs by using the antibodies. Protein microarrays have been used to assess protein-protein interactions, protein-ligand interactions, and autoantibody profiling in various disease conditions. Here, we summarize different microarray platforms with focus on its biological and clinical applications in autoantibody profiling and PTM studies. We also enumerate the potential of tissue microarrays to validate findings from protein arrays as well as other approaches, highlighting their significance in proteomics.

show abstract

Section: Application Of Protein Arrays In Ptm Studiesmentioning

confidence: 99%

Protein microarray applications: Autoantibody detection and posttranslational modification

et al. 2016

View full text Add to dashboard Cite

show abstract

Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures

Sekar

Taillefert

DiChristina

2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Improper disposal of 1,4-dioxane and the chlorinated organic solvents trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene [PCE]) has resulted in widespread contamination of soil and groundwater. In the present study, a previously designed microbially driven Fenton reaction system was reconfigured to generate hydroxyl (HO˙) radicals for simultaneous transformation of source zone levels of single, binary, and ternary mixtures of TCE, PCE, and 1,4-dioxane. The reconfigured Fenton reaction system was driven by fed batch cultures of the Fe(III)-reducing facultative anaerobe Shewanella oneidensis amended with lactate, Fe(III), and contaminants and exposed to alternating anaerobic and aerobic conditions. To avoid contaminant loss due to volatility, the Fe(II)-generating, hydrogen peroxide-generating, and contaminant transformation phases of the microbially driven Fenton reaction system were separated. The reconfigured Fenton reaction system transformed TCE, PCE, and 1,4-dioxane either as single contaminants or as binary and ternary mixtures. In the presence of equimolar concentrations of PCE and TCE, the ratio of the experimentally derived rates of PCE and TCE transformation was nearly identical to the ratio of the corresponding HO˙radical reaction rate constants. The reconfigured Fenton reaction system may be applied as an ex situ platform for simultaneous degradation of commingled TCE, PCE, and 1,4-dioxane and provides valuable information for future development of in situ remediation technologies. IMPORTANCE A microbially driven Fenton reaction system [driven by the Fe(III)-reducing facultative anaerobe S. oneidensis] was reconfigured to transform source zone levels of TCE, PCE, and 1,4-dioxane as single contaminants or as binary and ternary mixtures.The microbially driven Fenton reaction may thus be applied as an ex situ platform for simultaneous degradation of at least three (and potentially more) commingled contaminants. Additional targets for ex situ and in situ degradation by the microbially driven Fenton reaction developed in the present study include multiple combinations of environmental contaminants susceptible to attack by Fenton reaction-generated HO˙radicals, including commingled plumes of 1,4-dioxane, pentachlorophenol (PCP), PCE, TCE, 1,1,2-trichloroethane (TCA), and perfluoroalkylated substances (PFAS).T he chlorinated organic solvents trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene [PCE]) have been historically employed as solvents in a variety of industrial processes, including vapor degreasing of metal surfaces, paint stripping, and dry cleaning (1, 2). TCE and PCE are carcinogenic, and improper disposal practices at industrial sites have resulted in widespread contamination of soil and groundwater (1-6). Due to their high density and low aqueous phase solubility, TCE and PCE are also highly persistent in contaminated environments (3, 4). The potential carcinogen 1,4-dioxane is generally employed as a stabilizer for TCE and PCE i...

show abstract

Introducing glycophage arrays: Facile production, purification and patterning of glycophages

Cited by 2 publications

References 13 publications

Protein microarray applications: Autoantibody detection and posttranslational modification

Protein microarray applications: Autoantibody detection and posttranslational modification

Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures

Contact Info

Product

Resources

About