Introduction: 3D imaging in lung biology

Mühlfeld, Christian; Taatjes, Douglas J.

doi:10.1007/s00418-021-01968-z

Cited by 3 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 µm thick) with nm level axial resolution [ 152 ]. EM techniques are used extensively for structural imaging of the lung and to visualize cellular particle uptake and localization in different in vitro and ex vivo lung models [ 125 , 153 ]. TEM has been applied to visualize both the effects of PM exposure and to localize intracellular PM.…”

Section: High-resolution Imaging Of Respiratory Modelsmentioning

confidence: 99%

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

et al. 2023

View full text Add to dashboard Cite

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell–pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.

show abstract

Section: High-resolution Imaging Of Respiratory Modelsmentioning

confidence: 99%

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In addition to an overall reduced number of alveoli and alveolar surface, larger mean linear intercept (mean distance between two alveolar septae) and reduced radial alveolar count (fewer but larger alveoli) have been frequently reported [ 76 , 125 ], even though the latter two parameters are still debated. Therefore, it is of outstanding importance to analyze these heterogeneous structural alterations in the lungs using unbiased methods, for example, design-based stereology or whole-organ imaging approaches [ 126 , 127 ]. Further structural changes include an increased septal thickness, enhanced collagen and smooth muscle actin content, and diffuse distribution of elastin, all together impairing respiratory volume and function [ 11 , 60 , 119 , 128 , 129 ].…”

Section: Effect Of Hyperoxia On Vascular Formation and Structural Rem...mentioning

confidence: 99%

Perinatal Hyperoxia and Developmental Consequences on the Lung-Brain Axis

Obst

Herz

Alcázar

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Approximately 11.1% of all newborns worldwide are born preterm. Improved neonatal intensive care significantly increased survival rates over the last decades but failed to reduce the risk for the development of chronic lung disease (i.e., bronchopulmonary dysplasia (BPD)) and impaired neurodevelopment (i.e., encephalopathy of prematurity (EoP)), two major long-term sequelae of prematurity. Premature infants are exposed to relative hyperoxia, when compared to physiological in-utero conditions and, if needed to additional therapeutic oxygen supplementation. Both are associated with an increased risk for impaired organ development. Since the detrimental effects of hyperoxia on the immature retina are known for many years, lung and brain have come into focus in the last decade. Hyperoxia-induced excessive production of reactive oxygen species leading to oxidative stress and inflammation contribute to pulmonary growth restriction and abnormal neurodevelopment, including myelination deficits. Despite a large body of studies, which unraveled important pathophysiological mechanisms for both organs at risk, the majority focused exclusively either on lung or on brain injury. However, considering that preterm infants suffering from BPD are at higher risk for poor neurodevelopmental outcome, an interaction between both organs seems plausible. This review summarizes recent findings regarding mechanisms of hyperoxia-induced neonatal lung and brain injury. We will discuss common pathophysiological pathways, which potentially link both injured organ systems. Furthermore, promises and needs of currently suggested therapies, including pharmacological and regenerative cell-based treatments for BPD and EoP, will be emphasized. Limited therapeutic approaches highlight the urgent need for a better understanding of the mechanisms underlying detrimental effects of hyperoxia on the lung-brain axis in order to pave the way for the development of novel multimodal therapies, ideally targeting both severe preterm birth-associated complications.

show abstract

In focus in HCB

Taatjes

Roth

2022

Histochem Cell Biol

View full text Add to dashboard Cite

Introduction: 3D imaging in lung biology

Cited by 3 publications

References 39 publications

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

Perinatal Hyperoxia and Developmental Consequences on the Lung-Brain Axis

In focus in HCB

Contact Info

Product

Resources

About