Introduction: Antibody Structure and Function

Rajpal, Arvind; Strop, Pavel; Yeung, Yik Andy; Chaparro‐Riggers, Javier; Pons, Jaume

doi:10.1002/9783527675272.ch01

Cited by 7 publications

(10 citation statements)

References 213 publications

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“…Fcfusion products have demonstrated inferior stability compared to naturally existing antibodies and thus require additional stabilizers in their lyophilized formulations. The structural compatibility between Tc and Fc regions has been discussed among possible sources of instability [6,19]. Likewise, the conjugation of antibodies with small molecule pharmaceuticals results in less stable molecules than the original antibodies.…”

Section: Strategy For Different Modality Of Biologicsmentioning

confidence: 98%

“…These justifications must be based on relevant methods and results to evaluate the effect of the change as related to the product's safety or effectiveness (Appendix Regulatory Documents [18][19][20]. Although changes in degradation profiles after formulation modifications are undesirable, if the degradation profile changes qualitatively or quantitatively it is recommended to follow the impurity-related guidelines (Appendix Regulatory Documents 2,3).…”

Section: Strategy To Switch Formulationsmentioning

confidence: 99%

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Lyophilized Biologics

Chang¹,

Reilly²,

Chang³

2015

Lyophilized Biologics and Vaccines

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Section: Strategy For Different Modality Of Biologicsmentioning

confidence: 98%

Section: Strategy To Switch Formulationsmentioning

confidence: 99%

Lyophilized Biologics

Chang¹,

Reilly²,

Chang³

2015

Lyophilized Biologics and Vaccines

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“…Antibodies are composed of two light chains and two heavy chains that assemble into two antigen-binding regions and a single constant region in a Y-shaped configuration . There are two types of light chains (λ and κ) and five types of heavy chains (α, δ, ε, γ, and μ) .…”

Section: Introductionmentioning

confidence: 99%

Proteomic Characterization of Immunoglobulin Content in Dermal Interstitial Fluid

et al. 2019

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Microneedles have been demonstrated to be a minimally invasive technique for sampling dermal interstitial fluid (ISF). Shotgun quantitative proteomics has already identified hundreds of proteins in ISF and quantitatively compared the proteome to matching serum and plasma. Interstitial fluid was determined to be a viable minimally invasive alternative to blood-derived fluids. In this communication, we re-examined the proteomic data from previous work to determine the diversity of immunoglobulins present compared with serum and plasma. Similar to our previous findings regarding the proteomic content across fluid types, ISF had a similar composition of IgG, IgA, IgD, and IgE antibodies as plasma or serum and lower quantities of IgM, which reflects the relative concentrations of dermal tissue T-cell and B-cell populations, indicating that the Ig's were likely locally derived. This work has significant implications for the utility of measuring Ig's in ISF for the clinical diagnosis of immunological diseases and skin infections. Data are available via ProteomeXchange with identifier PXD012658.

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“…FAB is the antigen binding fragment which consists of variable region of heavy chain and light chain (VH and VL) and constant region [5]. Complementary determining region of variable region have loops involve in the interaction to the antigen [6,7].…”

Section: Introduction Vdj Recombinationmentioning

confidence: 99%

Recombinant Antibodies and Vaccines for Viruses

Rk¹

2019

J Biomol Res Ther

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Immunity produced when variable, diverse, and joining exons recombine and form diverse B-cell receptors. Somatic rearrangement of immunoglobulin genes termed VDJ recombination. This activity is controlled by RAG1 and RAG2 proteins, binds to the signal sequences and start cleavage. Double stranded breaks are produced by ROS, Nuclear enzymes and ATM. RAG protein induces cleavage activity. Segments exchange by CSR. After cleavage activity shuffling of segments occurs. TDT cause gain or loss of nucleotide bases. AID and RAG begins the process of CSR that shuffle exons of constant region. The core of NHEJ have catalytic subunit (DNA-Pkcs). Ku-DNA complex is important for the attachment of nuclease, polymerase and ligase of NHEJ. RAG2 with histone H3K4Me3 start recombination activity. HMG1 and HMG2 promote synapsis and cleavage. RNA Helicase of the DExD\H induces conformational changes. ZnA have ligase activity. Ku involved in the attachment of NHEJ factor DExD/H box who induce conformational changes. NHEJ machinery has XRCC4, XLF, and PAXX who ligate DNA ends. Protein kinase B and phosphoinositide-3 kinase involved in RNA expression. TOR69-3A2 is antibodies that neutralized Western equine encephalitis virus. AMMO1 is the anti gH/gL monoclonal antibody prevent to the Epstein bar virus infection. Antibodies also used for Ebola virus and Hepatitis. WT and HVR1 gpE1/gpE2 produce antibodies which target any type of cross-genotype neutralizing epitopes for HCV. GPE118, GPE325, GPE534 are targeted to different epitopes for Ebola virus.

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Introduction: Antibody Structure and Function

Cited by 7 publications

References 213 publications

Lyophilized Biologics

Lyophilized Biologics

Proteomic Characterization of Immunoglobulin Content in Dermal Interstitial Fluid

Recombinant Antibodies and Vaccines for Viruses

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