Introduction: Conservation of Small Carnivores

Glatston, Angela R.; Duplaix, Nicole

doi:10.1111/izy.12282

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Remote cameras have improved the quality of small carnivore surveys, but have limitations where morphologically similar species such as Asian otters co‐occur. There is an enormous lack of information about small carnivore distribution and ecology, with no robust and scientifically validated information on distribution ranges and population size for a number of species, owing to the lack of research studies (Glatston & Duplaix, 2020 ; Torres‐Romero & Giordano, 2022 ). Without this baseline information, assessment of the impacts and extent of threats to these species and the effectiveness of conservation measures is difficult.…”

Section: Discussionmentioning

confidence: 99%

Identification of three Asian otter species (Aonyx cinereus, Lutra sumatrana, and Lutrogale perspicillata) using a novel noninvasive PCR‐RFLP analysis

Sharma

Chee‐Yoong

Kannan

et al. 2022

Ecology and Evolution

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Four species of otters occur in tropical Asia, and all face multiple threats to their survival. Studies of distribution and population trends of these otter species in Asia, where they occur sympatrically, are complicated by their elusive nature and difficulties with reliable identification of species in field surveys. In Malaysia, only three species, the smooth‐coated otter, Asian small‐clawed otter, and hairy‐nosed otter have been reliably reported as residents. We designed a replicable and cost‐efficient PCR‐RFLP protocol to identify these three species. Using published reference sequences of mitochondrial regions, we designed and tested three PCR‐RFLP protocols on DNA extracted from reference samples and 33 spraints of wild otters collected along the North Central Selangor Coast of Malaysia. We amplified and sequenced two fragments (450 and 200 bp) of the mt D‐loop region and a 300‐bp fragment of the mt ND4 gene using primer sets TanaD, TanaD‐Mod, and OTR‐ND4, respectively. Amplification products were digested with restriction enzymes to generate species‐specific RFLP profiles. We analyzed the costs of all three protocols and compared these with the costs of sequencing for species identification. Amplification success was highest for the smallest PCR product, with the TanaD‐Mod primer amplifying DNA from all 33 spraints. TanaD and OTR‐ND4 primers amplified DNA from 60.6% and 63.6% spraints, respectively. PCR products of TanaD‐Mod provided the expected species‐specific RFLP profile for 32 (97%) of the spraints. PCR products of OTR‐ND4 provided the expected RFLP profile for all 21 samples that amplified, but TanaD produced spurious bands and inconsistent RFLP profiles. The OTR‐ND4 primer–enzyme protocol was the least expensive (437 USD) for processing 100 samples, followed by TanaD‐Mod (455 USD). We suggest the use of both OTR‐ND4 and TanaD‐Mod protocols that show potential for highly efficient and reliable species identification from noninvasive genetic sampling of three Asian otter species. We expect our novel noninvasive PCR‐RFLP analysis methods to facilitate population monitoring, ecological and behavioral studies on otters in tropical and subtropical Asia.

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Section: Discussionmentioning

confidence: 99%

Identification of three Asian otter species (Aonyx cinereus, Lutra sumatrana, and Lutrogale perspicillata) using a novel noninvasive PCR‐RFLP analysis

Sharma

Chee‐Yoong

Kannan

et al. 2022

Ecology and Evolution

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“…Since 2019, the status of small-clawed otters in CITES has been upgraded from Appendix II to Appendix I (Okamoto et al 2020;UNEP-WCMC 2021). According to Glatston and Duplaix (2020), illegal trade is hard to control because it is difficult to trace an individual's origin or identify the species. According to the Act of the Republic of Indonesia No.…”

Section: Introductionmentioning

confidence: 99%

The existence of Asian small-clawed otter (Aonyx cinereus Illiger, 1815) in the UCPS Hydropower, Cianjur, West Java, Indonesia

et al. 2021

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Abstract. Dirgantara AP, Megantara EN, Husodo T, Febrianto P, Wulandari I, Shanida SS. 2021. The existence of Asian small-clawed otter (Aonyx cinereus Illiger, 1815) in the UCPS Hydropower, Cianjur, West Java. Biodiversitas 22: 4391-4401. Little information about the existence of small-clawed otters (Aonyx cinereus) in Indonesia is available. Small-clawed otter is the top predator and biological indicator of wetland health. However, the small-clawed otter population in the world has decreased due to anthropogenic factors. This study aims to reveal 1) estimate the population size of small-clawed otters and 2) the small-clawed otter signs at the UCPS Hydropower construction site, Cianjur, West Java. This study used a qualitative approach in February-April 2017. Sign surveys (feces, footprints, leftovers, and dens) and camera trapping were applied in this study. There were 32 signs with details of 21 feces, eight footprints, and three dens. The estimation of small-clawed otter population size at UCPS Hydropower ranges from 5-14 individuals in total scattered in the Citali-Cisokan, Cilengkong, and Cirumamis rivers. In addition, the discovery of infant and bedding repair activities shows that the habitat in the UCPS Hydropower area supports the daily needs of small-clawed otters, especially in terms of breeding and shelter. Further studies are needed regarding the impact of the UCPS Hydropower construction on the existence of small-clawed otters, especially in the overlapping between small-clawed otter habitat and the UCPS Hydropower construction sites, by monitoring and making wildlife management policies.

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Introduction: Conservation of Small Carnivores

Cited by 2 publications

References 19 publications

Identification of three Asian otter species (Aonyx cinereus, Lutra sumatrana, and Lutrogale perspicillata) using a novel noninvasive PCR‐RFLP analysis

Identification of three Asian otter species (Aonyx cinereus, Lutra sumatrana, and Lutrogale perspicillata) using a novel noninvasive PCR‐RFLP analysis

The existence of Asian small-clawed otter (Aonyx cinereus Illiger, 1815) in the UCPS Hydropower, Cianjur, West Java, Indonesia

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