Introduction of a fungal NADP(H)-dependent glutamate dehydrogenase (<i>gdhA</i>) improves growth, grain weight and salt resistance by enhancing the nitrogen uptake efficiency in forage rice

Zhang, Haibo; Liang, Chen; Aoki, Naohiro; Kawai, Kiyoshi; Takane, Kenichi; Ohsugi, Ryu

doi:10.1080/1343943x.2015.1133237

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…GS is a major regulatory enzyme in ATPdependent assimilation of ammonium with glutamate (Zhang et al, 2016). The obtained glutamine was then incorporated with specific amino acids, producing precursors for other amino acids, proteins and nitrogen-containing compounds (Forde & Lea, 2007).…”

Section: Resultsmentioning

confidence: 99%

Biocontrol of wilt-inducing Fusarium oxysporum by aqueous leaf extract from Egyptian Ammi majus and Ammi visnaga

El-Sharkawy

Alshora

2020

Egypt. J. Bot.

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Section: Resultsmentioning

confidence: 99%

Biocontrol of wilt-inducing Fusarium oxysporum by aqueous leaf extract from Egyptian Ammi majus and Ammi visnaga

El-Sharkawy

Alshora

2020

Egypt. J. Bot.

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“…3 d). Lightfoot et al 58 found that gdhA transgenic plants had improved seed germination rate, growth level, and grain biomass production and showed enhanced plant resistance by promoting nitrogen uptake 59 . In our results, four glutamate dehydrogenase genes were induced by uniconazole under drought stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)

Jiang

Sun

Zheng

et al. 2021

Sci Rep

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Uniconazole (S-(+)-uniconazole), a plant growth retardant, exerts key roles in modulating growth and development and increasing abiotic stress tolerance in plants. However, the underlying mechanisms by which uniconazole regulates drought response remain largely unknown. Here, the effects of exogenous uniconazole on drought tolerance in hemp were studied via physiological and transcriptome analyses of the drought-sensitive industrial hemp cultivar Hanma No. 2 grown under drought stress. Exogenous uniconazole treatment increased hemp tolerance to drought-induced damage by enhancing chlorophyll content and photosynthesis capacity, regulating activities of enzymes involved in carbon and nitrogen metabolism, and altering endogenous hormone levels. Expression of genes associated with porphyrin and chlorophyll metabolism, photosynthesis-antenna proteins, photosynthesis, starch and sucrose metabolism, nitrogen metabolism, and plant hormone signal transduction were significantly regulated by uniconazole compared with that by control (distilled water) under drought stress. Numerous genes were differentially expressed to increase chlorophyll content, enhance photosynthesis, regulate carbon–nitrogen metabolism-related enzyme activities, and alter endogenous hormone levels. Thus, uniconazole regulated physiological and molecular characteristics of photosynthesis, carbon–nitrogen metabolism, and plant hormone signal transduction to enhance drought resistance in industrial hemp.

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“…PP2C9TL overexpressed the protein phosphatase that could dephosphorylate NR to keep active on nitrate availability. Glutamine synthetase (GS) catalyzes ammonia produced by photorespiration, protein catabolism, nitrate, and ammonia metabolism and takes part in N assimilation and transportation [ 79 , 80 ]. Glutamine synthetase (GS) protein (Spot 19) was upregulated in PP2C9TL relative to WT during low nitrogen.…”

Section: Discussionmentioning

confidence: 99%

Protein Phosphatase (PP2C9) Induces Protein Expression Differentially to Mediate Nitrogen Utilization Efficiency in Rice under Nitrogen-Deficient Condition

Waqas

Feng

Amjad

et al. 2018

IJMS

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Nitrogen (N) is an essential element usually limiting in plant growth and a basic factor for increasing the input cost in agriculture. To ensure the food security and environmental sustainability it is urgently required to manage the N fertilizer. The identification or development of genotypes with high nitrogen utilization efficiency (NUE) which can grow efficiently and sustain yield in low N conditions is a possible solution. In this study, two isogenic rice genotypes i.e., wild-type rice kitaake and its transgenic line PP2C9TL overexpressed protein phosphatase gene (PP2C9) were used for comparative proteomics analysis at control and low level of N to identify specific proteins and encoding genes related to high NUE. 2D gel electrophoresis was used to perform the differential proteome analysis. In the leaf proteome, 30 protein spots were differentially expressed between the two isogenic lines under low N level which were involved in the process of energy, photosynthesis, N metabolism, signaling, and defense mechanisms. In addition, we have found that protein phosphatase enhances nitrate reductase activation by downregulation of SnRK1 and 14-3-3 proteins. Furthermore, we showed that PP2C9TL exhibits higher NUE than WT due to higher activity of nitrate reductase. This study provides new insights on the rice proteome which would be useful in the development of new strategies to increase NUE in cereal crops.

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Introduction of a fungal NADP(H)-dependent glutamate dehydrogenase (gdhA) improves growth, grain weight and salt resistance by enhancing the nitrogen uptake efficiency in forage rice

Cited by 4 publications

References 38 publications

Biocontrol of wilt-inducing Fusarium oxysporum by aqueous leaf extract from Egyptian Ammi majus and Ammi visnaga

Biocontrol of wilt-inducing Fusarium oxysporum by aqueous leaf extract from Egyptian Ammi majus and Ammi visnaga

Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)

Protein Phosphatase (PP2C9) Induces Protein Expression Differentially to Mediate Nitrogen Utilization Efficiency in Rice under Nitrogen-Deficient Condition

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