Introduction of a putative biocontrol agent into a range of phytoplasma‐ and liberibacter‐susceptible crop plants

Lidor, Ofir; Dror, Orit; Hamershlak, Dor; Shoshana, Nofar; Belausov, Eduard; Zahavi, Tirtza; Mozes-Daube, Netta; Naor, Vered; Zchori-Fein, Einat; Iasur-Kruh, Lilach; Bahar, Ofir

doi:10.1002/ps.4775

Cited by 13 publications

(11 citation statements)

References 36 publications

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“…For instance, they have been proposed as probiotics for honeybees to sustain insect health against specific pathogens ( Crotti et al, 2013 ; Daisley et al, 2019 ) or as beneficial strains to improve the fitness of sterile male insects vs. conventional ones ( Ben Ami et al, 2010 ; Augustinos et al, 2015 ). Recently, insect symbionts have been suggested as biocontrol agents against phloem-limited pathogens ( Iasur-Kruh et al, 2016 , 2018 ; Gonella et al, 2018 , 2019 ; Lidor et al, 2018 ). In this work, we focused on the bacterial gut inhabitants of the BSF larvae fed on a nutritionally complete diet, on which BSF performance and fitness were previously showed to be higher than on nutritionally poor diets ( Jucker et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Hydrolytic Profile of the Culturable Gut Bacterial Community Associated With Hermetia illucens

et al. 2020

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Larvae of the black soldier fly (BSF) Hermetia illucens (L.) convert organic waste into high valuable insect biomass that can be used as alternative protein source for animal nutrition or as feedstock for biodiesel production. Since insect biology and physiology are influenced by the gut microbiome, knowledge about the functional role of BSFassociated microorganisms could be exploited to enhance the insect performance and growth. Although an increasing number of culture-independent studies are unveiling the microbiota structure and composition of the BSF gut microbiota, a knowledge gap remains on the experimental validation of the contribution of the microorganisms to the insect growth and development. We aimed at assessing if BSF gut-associated bacteria potentially involved in the breakdown of diet components are able to improve host nutrition. A total of 193 bacterial strains were obtained from guts of BSF larvae reared on a nutritious diet using selective and enrichment media. Most of the bacterial isolates are typically found in the insect gut, with major representatives belonging to the Gammaproteobacteria and Bacilli classes. The hydrolytic profile of the bacterial collection was assessed on compounds typically present in the diet. Finally, we tested the hypothesis that the addition to a nutritionally poor diet of the two isolates Bacillus licheniformis HI169 and Stenotrophomonas maltophilia HI121, selected for their complementary metabolic activities, could enhance BSF growth. B. licheniformis HI169 positively influenced the larval final weight and growth rate when compared to the control. Conversely, the addition of S. maltophilia HI121 to the nutritionally poor diet did not result in a growth enhancement in terms of larval weight and pupal weight and length in comparison to the control, whereas the combination of the two strains positively affected the larval final weight and the pupal weight and length. In conclusion, we isolated BSF-associated bacterial strains with potential positive properties for the host nutrition and we showed that selected isolates may enhance BSF growth, suggesting the importance to evaluate the effect of the bacterial administration on the insect performance.

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Section: Discussionmentioning

confidence: 99%

Hydrolytic Profile of the Culturable Gut Bacterial Community Associated With Hermetia illucens

et al. 2020

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“…Once endophytes enter into the plant, they move systemically (Nigris et al, 2018), spreading in different tissues as shown in Figure 2 , where green fluorescent protein (GFP)-tagged Bacillus licheniformis can be visualized within the stem of a grapevine-inoculated plant. Indeed, endophytes can colonize other tissues and organs, including reproductive organs, thus allowing their transfer through the vascular system (Lidor et al, 2018) or apoplastic spaces (Compant et al, 2011). An analysis of different aerial portions of grapevines was carried out and revealed differential colonization by endophytes; both Pseudomonas and Bacillus were found within the flower and ovules, while only the latter was present in the berries and seeds (Compant et al, 2011).…”

Section: Biodiversitymentioning

confidence: 99%

The Role of the Endophytic Microbiome in the Grapevine Response to Environmental Triggers

et al. 2019

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Endophytism within Vitis represents a topic of critical relevance due to the multiple standpoints from which it can be approached and considered. From the biological and botanical perspectives, the interaction between microorganisms and perennial woody plants falls within the category of stable relationships from which the plants can benefit in multiple ways. The life cycle of the host ensures persistence in all seasons, repeated chances of contact, and consequent microbiota accumulation over time, leading to potentially high diversity compared with that of herbaceous short-lived plants. Furthermore, grapevines are agriculturally exploited, highly selected germplasms where a profound man-driven footprint has indirectly and unconsciously shaped the inner microbiota through centuries of cultivation and breeding. Moreover, since endophyte metabolism can contribute to that of the plant host and its fruits’ biochemical composition, the nature of grapevine endophytic taxa identities, ecological attitudes, potential toxicity, and clinical relevance are aspects worthy of a thorough investigation. Can endophytic taxa efficiently defend grapevines by acting against pests or confer enough fitness to the plants to endure attacks? What are the underlying mechanisms that translate into this or other advantages in the hosting plant? Can endophytes partially redirect plant metabolism, and to what extent do they act by releasing active products? Is the inner microbial colonization necessary priming for a cascade of actions? Are there defined environmental conditions that can trigger the unleashing of key microbial phenotypes? What is the environmental role in providing the ground biodiversity by which the plant can recruit microsymbionts? How much and by what practices and strategies can these symbioses be managed, applied, and directed to achieve the goal of a better sustainable viticulture? By thoroughly reviewing the available literature in the field and critically examining the data and perspectives, the above issues are discussed.

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“…In conclusion, phloem-limited plant pathogenic bacteria such as Phytoplasma, Spiroplasma, and Liberibacter pose a major threat to a list of agricultural crops [12].…”

Section: The Effect Of Qc and Hmf On Phytoplasma In Periwinklementioning

confidence: 99%

“…However, unlike phytoplasma that inhabits the insect midgut, hemocoel, and salivary glands, F. defendens was found only in the insect gut, suggesting that it was not vertically transmitted, but rather acquired from the plant [11]. Indeed, upon foliage application, F. defendens was shown to penetrate the leaf surface and inhabit the vascular tissues of various plant crops including grapevine, orange, pomelo, cotton, cucumber, and carrot, with no apparent phytotoxic effect [12]. Further research showed that a supernatant of F. defendens culture inhibits the culture growth of Spiroplasma melliferum (Entomoplasmatales, Spiroplasmataceae), which is a cultivable model of phytopathogenic Mollicutes [9]; the secretion of metabolites, which directly inhibit phytoplasma growth, was suggested as one possible mechanism by which F. defendens may reduce yellows symptoms.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium Frateuria defendens

Naama-Amar

Gitman

Shoshana

et al. 2020

Plants

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Candidatus Phytoplasma, the causative agent of yellows disease, inflicts substantial damage on several hundred plant species including perennials and annual plants. The endophytic bacterium Frateuria defendens reduces the symptoms of yellows disease in a number of agricultural crops. One possible mode of action is that the bacterium secretes antimicrobial metabolites. To test this hypothesis, the substances secreted by the endophyte during 10 days of growth in an artificial medium were identified by GC-MS (gas chromatography–mass spectrometry). Synthetic analogues to these substances were then used on periwinkle, a nurse culture plant infected by phytoplasma. Phytoplasma quantities were evaluated by quantitative PCR, and disease symptoms were monitored and recorded. It was found that specific compounds identified by the biochemical analysis caused a significant reduction in both the titer of phytoplasma and the disease symptoms in periwinkle when compared to untreated infected plants. Further research is required to examine the potential of these compounds as an effective treatment against yellows disease.

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Introduction of a putative biocontrol agent into a range of phytoplasma‐ and liberibacter‐susceptible crop plants

Abstract: DLB is a potential biocontrol agent and its broad spectrum of host plants indicates the possibility of its future use against a range of diseases caused by phloem-limited bacteria. © 2017 Society of Chemical Industry.

Cited by 13 publications

References 36 publications

Hydrolytic Profile of the Culturable Gut Bacterial Community Associated With Hermetia illucens

Hydrolytic Profile of the Culturable Gut Bacterial Community Associated With Hermetia illucens

The Role of the Endophytic Microbiome in the Grapevine Response to Environmental Triggers

Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium Frateuria defendens

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