Introduction of Flocculation Into an Industrial Yeast Strain by Transfer of a Single Chromosome

Vezinhet, Françoise; Barré, Pierre; Laurent, Monique; Valade, Michel

doi:10.1002/j.2050-0416.1992.tb01114.x

Cited by 8 publications

(2 citation statements)

References 5 publications

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“…Kar1 mutant-mediated abortive mating allows the transfer of a single chromosome from one parent to another while losing the remaining chromosomes. ,,, To test the ability of the chromosome transfer method for delivering synthetic yeast chromosomes to nonsynthetic hosts, synthetic chromosomes III, V, X, and XII were respectively transferred into BY4741 or BY4742 based on their mating types (Figure a). To construct a kar1Δ15 mutant (deletion of the region between residues 106 and 192) for the essential gene KAR1 , a two-step method was applied in the wild-type strain BY4741, the synV strain (yML007), and the synX strain (yML004).…”

Section: Resultsmentioning

confidence: 99%

“…The essential gene KAR1 is involved in karyogamy during yeast mating, , and mutations of the KAR1 genes in parent strains can delay the process of nuclear fusion . In the progeny of zygote, occasionally one or more chromosomes are transferred from one parental nucleus to another. , This kar1 mutant-mediated method has been used to transfer YAC plasmids or to generate disomic yeast strains. − Here, using kar1 mutant-mediated abortive mating strategy, synthetic yeast chromosomes were directly transferred to wild-type strains BY4741 and an industrial strain Y12. Four synthetic yeast chromosomes (synIII, synV, synX, synXII) were separately transferred to BY4741, resulting in various tolerant traits.…”

Section: Introductionmentioning

confidence: 99%

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Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination

Guo

Yin

et al. 2022

ACS Synth. Biol.

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Large DNA transfer technology has been challenged with the rapid development of large DNA assembly technology. The research and application of synthetic yeast chromosomes have been mostly limited in the assembled host itself. The mutant of KAR1 prevents nuclear fusion during yeast mating, and occasionally single chromosome can be transferred from one parental nucleus to another. Using the kar1 mutant method, four synthetic yeast chromosomes of Sc2.0 (synIII, synV, synX, synXII) were transferred to wild-type yeasts separately. SynIII was also transferred into an industrial strain Y12, resulting in an improvement of thermotolerance. Moreover, by combining abortive mating and chromosome elimination by CRISPR-Cas9, which has been reported in our previous study, we developed a strategy for consolidation of multiple synthetic yeast chromosomes. Compared to the previous pyramidal strategy using endoreduplication backcross, our method is a linear process independent of meiosis, providing a convenient path for accelerating consolidation of Sc2.0 chromosomes. Overall, the method of transfer and consolidation of synthetic yeast chromosomes by abortive mating and chromosome elimination enables a novel route that large DNA was assembled in donor yeast and then in vivo directly transferred to receptor yeasts, enriching the manipulation tools for synthetic genomics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination

Guo

Yin

et al. 2022

ACS Synth. Biol.

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Another brick in the wall? Recent developments concerning the yeast cell envelope

Stratford

1994

Yeast

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To a yeast, the cell wall is an important living organelle performing a number of vital functions, including osmotic and physical protection, selective permeability barrier, immobilized enzyme support and cell-cell recognition and adhesion. Our basic model of wall structure involves attachment of secreted mannoproteins to a fibrillar inner layer of beta-glucan. Recent work has emphasised the importance of chitin in lateral walls, examined the mechanisms of attachment of mannoproteins to the various cell wall glucan fractions and elucidated the pathway of beta-glucan synthesis, by means of resistance to glucan-binding killer toxins. The conventional view of wall structure has been challenged by the discovery of a class of GPI-anchored, serine/threonine-rich wall-proteins. It has been suggested, that these proteins are anchored in the plasma membrane, spanning the wall with extended O-glycosylated structures and protruding out into the medium. Examination of these proteins shows a diversity of structures, sizes and behaviour that makes it improbable that these represent a new class of wall proteins. The possible roles of one of these proteins associated with flocculation, Flo1p, are discussed.

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Yeasts and Sparkling Wine Production

Alexandre

2019

Yeasts in the Production of Wine

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Introduction of Flocculation Into an Industrial Yeast Strain by Transfer of a Single Chromosome

Cited by 8 publications

References 5 publications

Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination

Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination

Another brick in the wall? Recent developments concerning the yeast cell envelope

Yeasts and Sparkling Wine Production

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