Introduction of ligated vessels promote the retention and regeneration of free fat: constructing a fat flap in tissue engineering chamber

Chen, Lei; Cai, Beichen; Chen, Xiaobin; Huang, Zhiyong; Wang, Biao

doi:10.1080/21623945.2020.1735025

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…A further challenge to the TEC method was made by Lei et al 26 They inserted ligated vessels with fat grafts into a chamber, using fat grafts instead of less available native flaps. This study demonstrated that ligated vessels can serve as a nourishing source to fat flap creation inside a TEC.…”

Section: Tissue Engineering Chambermentioning

confidence: 99%

Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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Background: Currently, the gold standard for complex defect reconstruction is autologous tissue flaps, with vascularized composite allografts as its highest level. Good clinical results are obtained despite considerable obstacles, such as limited donor sites, donor site morbidity, and complex operations. Researchers in the field of tissue engineering are trying to generate novel tissue flaps requiring small or no donor site sacrifice. At the base of existing technologies is the tissue’s potential for regeneration and neovascularization. Methods: A review was conducted identifying relevant published articles in PubMed on the subject of flap engineering, with the focus on plastic surgery. This review article surveys contemporary technologies in flap engineering, including cell sheet technology, prefabricated flaps, and tissue engineering chambers. Conclusions: Some of the described procedures, though not yet ready for clinical use, are certainly ready for trial in large animal models and even human studies. Tissue engineering is a promising field for the handling of large and complex tissue defects.

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Section: Tissue Engineering Chambermentioning

confidence: 99%

Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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“…These drawbacks can be resolved by restoration of the breast shape through tissue engineering. Formation of a stable soft tissue on the recipient site requires sufficient vascularization and adipose tissue regeneration [ 2 , 3 ], and this is a major challenge in breast tissue engineering. Stem cells act as seed cells in tissue engineering whereby they can regenerate expected tissue via direct differentiation into proper cell type and paracrine regulation [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cell promotes angiogenesis via integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway for off-the-shelf breast tissue engineering

Chen

et al. 2022

Stem Cell Res Ther

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Background Mesenchymal stem cells (MSC)-based tissue engineered breast represent the visible future for breast reconstruction after mastectomy. However, autologous MSCs might not be appropriate for the large graft construction due to cell senescence during excessive cell expansion, thus hindering its further off-the-shelf application. The human umbilical cord mesenchymal stem cells (hUCMSCs) have been found to induce low immune response and can be easily stored, making them ideal for off-the-shelf tissue engineering application. Here, we explored the feasibility of using umbilical cord mesenchymal stem cells as tissue-engineered breast seed cells. Methods The allogenic hUCMSCs were injected into transplanted fat tissue with or without breast scaffolds as an alternative for breast tissue engineering in vivo, and its potential mechanism of angiogenesis in vitro was explored. Results Transplantation of hUCMSCs promoted proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs) through paracrine mechanism by activating the integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway. Histological examination of grafted fat revealed that the group which received hUCMSCs transplantation had more fat tissue [(93.60 ± 2.40) %] and fewer MAC2+CD206− M1 macrophages [(0.50 ± 0.47) cells/field] compared to the control group [fat tissue (45.42 ± 5.96) and macrophage cells/field (5.00 ± 2.23)]. Moreover, the hUCMSCs- labeled with a tracing dye differentiated into adipocytes and vascular endothelial cells in the adipose tissue. When applied to the tissue-engineered breast with a scaffold, the group treated with hUCMSCs had more adipose tissues and CD31+ cells than the control group. Conclusions These results demonstrate that allogeneic hUCMSCs promote the regeneration of adipose tissue and can be used to construct a tissue engineered breast.

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Clinically Relevant and Precisely Printable Live Adipose Tissue–Based Bio‐Ink for Volumetric Soft Tissue Reconstruction

Jeong,

Son,

Choi

et al. 2024

Adv Healthcare Materials

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Autologous fat is widely used in soft tissue reconstruction; however, significant volume reduction owing to necrosis and degradation of the transplanted adipose tissue (AT) remains a major challenge. To address this issue, a novel live AT micro‐fragment–based bio‐ink (ATmf bio‐ink) compatible with precision 3D printing, is developed. Live AT micro‐fragments of ≈280 µm in size are prepared using a custom tissue micronizer and they are incorporated into a fibrinogen/gelatin mixture to create the ATmf bio‐ink. AT micro‐fragments exhibit high viability and preserve the heterogeneous cell population and extracellular matrix of the native AT. The developed bio‐ink enables precise micropatterning and provides an excellent adipo‐inductive microenvironment. AT grafts produced by co‐printing the bio‐ink with polycaprolactone demonstrate a 500% improvement in volume retention and a 300% increase in blood vessel infiltration in vivo compared with conventional microfat grafts. In vivo engraftment of AT grafts is further enhanced by using a stem cell–laden ATmf bio‐ink. Last, it is successfully demonstrated that the bio‐ink is enabled for the creation of clinically relevant and patient‐specific AT grafts for patients undergoing partial mastectomy. This novel ATmf bio‐ink for volumetric soft tissue reconstruction offers a pioneering solution for addressing the limitations of existing clinical techniques.

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Introduction of ligated vessels promote the retention and regeneration of free fat: constructing a fat flap in tissue engineering chamber

Cited by 4 publications

References 38 publications

Techniques and Innovations in Flap Engineering: A Review

Techniques and Innovations in Flap Engineering: A Review

Human umbilical cord mesenchymal stem cell promotes angiogenesis via integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway for off-the-shelf breast tissue engineering

Clinically Relevant and Precisely Printable Live Adipose Tissue–Based Bio‐Ink for Volumetric Soft Tissue Reconstruction

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