Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion

Abstract

Interspecific protoplast fusion between two monokaryotic strains (a methionine auxotrophic and chloramphenicol-resistant Pleurotus ostreatus strain and a wild type strain of Pleurotus pulmonarius) was carried out to introduce mitochondrial DNA (mtDNA) from P. ostreatus into P. pulmonarius. Because mycelial colonies 1 regenerated on minimum medium containing chloramphenicol only after the treatment of protoplast electrofusion, the regenerants were regarded as protoplast fusants. The fusants isolated from regenerated colonies were uninucleate, and their resistance of chloramphenicol seemed to be a stable trait. The fusants mated with P. pulmonarius but not with P. ostreatus, and showed almost identical RAPD patterns to that of the parental P. pulmonarius monokaryon. The sizes of mitochondrial genomes were estimated to be 81.5kb for P. ostreatus monokaryon, 54.9 kb for P. pulmonarius monokaryon, and 84.5 or 86.0 kb for the fusants. BamHI and EcoRI digest patterns of the fusant mtDNAs were almost similar to the parental P. ostreatus monokaryon. From the above results, the fusants seemed to carry nuclei derived from the monokaryon of P. pulmonarius and mtDNA including chloramphenicol-resistance gene from the P. ostreatus monokaryon, suggesting that the P. ostreatus mtDNA had been introduced into P. pulmonarius cells by interspecific protoplast fusion.2

Improving biological efficiency of Pleurotus strain through protoplast fusion between P. ostreatus var. florida and P. djamor var. roseus

“…Fukuda et al (4) have reported the successful mitochondrial DNA transmission in interspecific fusion protoplasts of edible mushrooms Pleurotus ostreatu (amethionine auxotrophic and chloramphenicol-resistant strain) and Pleurotus pulmonarius (wildtype strain), thus increasing the genetic variability of economically important mushrooms. This possibility is useful in mushroom breeding, because the mitochondrial genome may affect phenotypical properties of edible mushrooms.…”

Interspecies hybridization between Auricularia cornea cv. Yu Muer and Auricularia heimuer cv. Bai Muer through protoplast fusion

“…In addition, protoplast fusion may result in interactions between nuclear and exonuclear genes, such as mitochondrial genes (Stasz and Harman, 1990;Harman and Stasz, 1991;Harman and Hayes, 1993). Fukuda has reported the successful mitochondrial DNA transmission in interspecific fusion protoplasts of Pleurotus, which increased the genetic variability of economically significant mushrooms (Fukuda et al, 2007). Because mitochondrial genomes may influence the phenotypic characteristics of edible mushrooms, this possibility is useful in mushroom breeding (Zhao and Chang, 1997).…”