Introduction of N-Linked Glycans in the Lectin Domain of Surfactant Protein D

Eijk, Martin van; Bruinsma, Laurie; Hartshorn, Kevan L.; White, Mitchell R.; Rynkiewicz, Michael J.; Seaton, Barbara A.; Hemrika, Wieger; Romijn, Roland A.; Balkom, Bas Wm van; Haagsman, Henk P.

doi:10.1074/jbc.m111.224469

Cited by 22 publications

(23 citation statements)

References 45 publications

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“…For this purpose, we applied the HEK293 expression system that was previously used successfully for the production of full-length wild-type pSP-D, wild-type hSP-D, and various h/pSP-D derivatives (26). Biochemical characterization showed that both RpNCRD and RpNCRD-dNG were expressed as trimeric proteins, and N-glycanase digestions confirmed the absence of N-linked glycans in the RpNCRD-dNG preparation.…”

Section: Discussionmentioning

confidence: 99%

“…Chemical crosslinking of RpNCRD and RpNCRD-dNG was achieved with a BS3 cross-linker as described previously (26).…”

Section: Methodsmentioning

confidence: 99%

“…Construction of pSP-D-NCRD cDNA-A full-length pSP-D cDNA clone in pCR4-TOPO vector (26) was used as a template in a standard 50-l PCR using proofstart DNA polymerase to amplify the NCRD region of pSP-D starting at the codon coding for Gly-223 (numbers according to Ref. 20) and ending 142 nucleotides downstream from the stop codon (total length, 613 bp).…”

Section: Methodsmentioning

confidence: 99%

“…Endotoxin values of RhNCRD preparations were below 0.1 pg/g protein. Recombinant full-length porcine SP-D (RpSP-D) was produced and purified as described (26).…”

Section: Methodsmentioning

confidence: 99%

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A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D

Eijk

Rynkiewicz

White

et al. 2012

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

“…Chemical crosslinking of RpNCRD and RpNCRD-dNG was achieved with a BS3 cross-linker as described previously (26).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Endotoxin values of RhNCRD preparations were below 0.1 pg/g protein. Recombinant full-length porcine SP-D (RpSP-D) was produced and purified as described (26).…”

Section: Methodsmentioning

confidence: 99%

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A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D

Eijk

Rynkiewicz

White

et al. 2012

Journal of Biological Chemistry

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“…It is speculated that the SGA‐loop region is involved in generating enhanced binding properties of the CRD of pSP‐D to viral glycans. In addition, site‐directed mutagenesis studies with recombinant human SP‐D have also indicated that this porcine‐specific SGA‐loop is likely to be involved in mediating the antiviral activity of the N‐linked glycan in the CRD of pSP‐D [65].…”

Section: Collectinsmentioning

confidence: 99%

The carbohydrate recognition domain of collectins

2011

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Collectins are effector molecules of the innate immune system that play an important role in the first line of defence against bacteria, viruses and fungi. Most of their interactions with microorganisms are mediated through their carbohydrate recognition domain (CRD), which binds in a Ca2+‐dependent manner to glycoconjugates. This domain is a well‐known structure that is present in a larger group of proteins comprising the C‐type lectin domain family. Collectins form a subgroup within this family based on the presence of a collagen domain and the trimerization of CRDs, which are essential for the ligand‐binding properties of these proteins. The ligand specificity among the nine collectin members is significantly different as a result of both the structural organization of the trimers and specific sequence changes in the binding pocket of the CRD. In addition, some collectin members have additional features, such as N‐linked glycosylation of CRD residues and additional loop structures within the CRD that have a large impact on their interaction with the glycoconjugates present on microorganisms or host cells. The availability of crystal structures of three members of the collectin family (surfactant proteins A and D and mannan‐binding protein) provides an important tool for addressing the impact of these CRD differences on ligand binding. In this review, the structural differences and similarities between the CRDs of collectins are summarized and their relationship with their ligand‐binding characteristics is discussed.

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Emerging Antiviral Strategies to Interfere with Influenza Virus Entry

2013

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Influenza A and B viruses are highly contagious respiratory pathogens with a considerable medical and socioeconomical burden and known pandemic potential. Current influenza vaccines require annual updating and provide only partial protection in some risk groups. Due to the global spread of viruses with resistance to the M2 proton channel inhibitor amantadine or the neuraminidase inhibitor oseltamivir, novel antiviral agents with an original mode of action are urgently needed. We here focus on emerging options to interfere with the influenza virus entry process, which consists of the following steps: attachment of the viral hemagglutinin to the sialylated host cell receptors, endocytosis, M2-mediated uncoating, low pH-induced membrane fusion, and, finally, import of the viral ribonucleoprotein into the nucleus. We review the current functional and structural insights in the viral and cellular components of this entry process, and the diverse antiviral strategies that are being explored. This encompasses small molecule inhibitors as well as macromolecules such as therapeutic antibodies. There is optimism that at least some of these innovative concepts to block influenza virus entry will proceed from the proof of concept to a more advanced stage. Special attention is therefore given to the challenging issues of influenza virus (sub)type-dependent activity or potential drug resistance.

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Introduction of N-Linked Glycans in the Lectin Domain of Surfactant Protein D

Cited by 22 publications

References 45 publications

A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D

A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D

The carbohydrate recognition domain of collectins

Emerging Antiviral Strategies to Interfere with Influenza Virus Entry

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