Introduction of phospholipids to cultured cells with cyclodextrin

Efficient replacement of plasma membrane outer leaflet phospholipids and sphingolipids in cells with exogenous lipids

“…CDs, cyclic oligomers of glucose with a hydrophobic cavity, have been long used to transfer and modify cell sterols (9), but their use to exchange sphingolipids and phospholipids in cells has been little explored. Prior studies have shown that phospholipids can be delivered into cells by methyl-β-cyclodextrin (MβCD), and that delivered lipids are not rapidly turned over (10,11). However, the amount of exchange was low, except for lipids with short acyl chains.…”

Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice

“…The correct insertion of the respective targeting vector into the SMSr locus was verifi ed by Southern blot hybridization as described previously ( 35 ), using genomic DNA from the liver by guest, on www.jlr.org ethanolamine (PE), phosphatidylserine] scanning as described previously ( 41 ). The data were analyzed with Microsoft Excel using the LIMSA add-on ( 42 ).…”

Interaction of fluorescent phospholipids with cyclodextrins

“…As stated above, the role of the lipid head groups should be minor since one would expect only a limited interaction, if at all, with the hydrophobic CD cavities. In line with this, the MbCD-mediated transfer of isotope-labeled phospholipids between vesicles did not depend on the structure of the head groups but rather on the hydrophobicity of the acyl chains (Kainu et al, 2010). Therefore, the binding of CDs to (endogenous) phospholipids should be mediated via an interaction with the fatty acyl chains.…”

“…CDs are also able to bind phospholipids (Ohtani et al, 1989;Puglisi et al, 1996;Rawyler and Siegenthaler, 1996;Fauvelle et al, 1997;Debouzy et al, 1998;Nishijo and Mizuno, 1998;Nishijo et al, 2000;Leventis and Silvius, 2001;Duchêne and Siegenthaler, 2002;Anderson et al, 2004;Giocondi et al, 2004;Hatzi et al, 2007;Piel et al, 2007;Kainu et al, 2010;Huang and London, 2013). This interaction has a different CD-specificity compared with that observed for sterols.…”

“…This interaction has a different CD-specificity compared with that observed for sterols. Somerharju and coworkers have characterized the binding of endogenous phospholipids to MßCD and applied those CD-lipid complexes to successfully incorporate endogenous phospholipids into plasma membranes (Kainu et al, 2010). The incorporation of phospholipids with long chain fatty acyl chains into membranes is a challenging task.…”