Introduction of sense constructs of cinnamate 4-hydroxylase (CYP73A24) in transgenic tomato plants shows opposite effects on flux into stem lignin and fruit flavonoids

Millar, David J.; Long, Marianne T.; Donovan, Georgina; Fraser, Paul D.; Boudet, Alain‐Michel; Danoun, Saı̈da; Bramley, Peter M.; Bolwell, G. Paul

doi:10.1016/j.phytochem.2007.03.018

Cited by 39 publications

(21 citation statements)

References 30 publications

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“…The PKMF model predicts that a severe reduction in the C4H protein abundance results in a reduction in lignin content. The lignin content of C4H transgenic plants of tobacco (Sewalt et al, 1997), alfalfa (Reddy et al, 2005), tomato (Solanum lycopersicum; Millar et al, 2007), Arabidopsis (Schilmiller et al, 2009), and hybrid aspen (Populus tremula 3 tremuloides; Bjurhager et al, 2010) is consistent with our predictions. The S/G ratios of C4H transgenic plants are inconsistent.…”

Section: The Pkmf Model Strengthens Previous Knowledge and Predicts Rsupporting

confidence: 88%

Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa

et al. 2014

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We established a predictive kinetic metabolic-flux model for the 21 enzymes and 24 metabolites of the monolignol biosynthetic pathway using Populus trichocarpa secondary differentiating xylem. To establish this model, a comprehensive study was performed to obtain the reaction and inhibition kinetic parameters of all 21 enzymes based on functional recombinant proteins. A total of 104 Michaelis-Menten kinetic parameters and 85 inhibition kinetic parameters were derived from these enzymes. Through mass spectrometry, we obtained the absolute quantities of all 21 pathway enzymes in the secondary differentiating xylem. This extensive experimental data set, generated from a single tissue specialized in wood formation, was used to construct the predictive kinetic metabolic-flux model to provide a comprehensive mathematical description of the monolignol biosynthetic pathway. The model was validated using experimental data from transgenic P. trichocarpa plants. The model predicts how pathway enzymes affect lignin content and composition, explains a longstanding paradox regarding the regulation of monolignol subunit ratios in lignin, and reveals novel mechanisms involved in the regulation of lignin biosynthesis. This model provides an explanation of the effects of genetic and transgenic perturbations of the monolignol biosynthetic pathway in flowering plants.

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Section: The Pkmf Model Strengthens Previous Knowledge and Predicts Rsupporting

confidence: 88%

Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa

et al. 2014

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“…Chlorogenic acid was increased in plants with higher PAL activity while the flavonoid rutin was not increased. Over-expression of C4H in tomato gave rise to some plants with increased rutin and naringenin, though increased level of those compounds was not directly correlated to increased level of C4H (Millar et al 2007). Some reports revealed an increase in flavonoids by activating transcription factors.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that overexpression of RsTAL has no visibly adverse effect on plants. In some cases, altered accumulation levels of secondary metabolites cause adverse effects on growth, morphology, and fertility (Ylstra et al 1994;Fischer et al 1997;Sharma and Dixon 2005;Millar et al 2007). Normal growth and fertility are advantageous characteristics in metabolic engineering.…”

Section: Discussionmentioning

confidence: 99%

Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis

Nishiyama

Yun

Mizutani

et al. 2010

Planta

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Some flavonoids are considered as beneficial compounds because they exhibit anticancer or antioxidant activity. In higher plants, flavonoids are secondary metabolites that are derived from phenylpropanoid biosynthetic pathway. A large number of phenylpropanoids are generated from p-coumaric acid, which is a derivative of the primary metabolite, phenylalanine. The first two steps in the phenylpropanoid biosynthetic pathway are catalyzed by phenylalanine ammonia-lyase and cinnamate 4-hydroxylase, and the coupling of these two enzymes forms a rate-limiting step in the pathway. For the generation of p-coumaric acid, the conversion from phenylalanine to p-coumaric acid that is catalyzed by two enzymes can be theoretically performed by a single enzyme, tyrosine ammonia-lyase (TAL) that catalyzes the conversion of tyrosine to p-coumaric acid in certain bacteria. To modify the p-coumaric acid pathway in plants, we isolated a gene encoding TAL from a photosynthetic bacterium, Rhodobacter sphaeroides, and introduced the gene (RsTAL) in Arabidopsis thaliana. Analysis of metabolites revealed that the ectopic over-expression of RsTAL leads to higher accumulation of anthocyanins in transgenic 5-day-old seedlings. On the other hand, 21-day-old seedlings of plants expressing RsTAL showed accumulation of higher amount of quercetin glycosides, sinapoyl and p-coumaroyl derivatives than control. These results indicate that ectopic expression of the RsTAL gene in Arabidopsis enhanced the metabolic flux into the phenylpropanoid pathway and resulted in increased accumulation of flavonoids and phenylpropanoids.

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“…In the present time, the social demand and an increasing interest for practical applications has stimulated a wide range of biological and epidemiological studies aiming at characterizing the health-promoting properties of specific phenolic compounds with antioxidant activities towards cancer, cardiovascular and neurodegenerative diseases, or for use in anti-aging or cosmetic products (Millar et al 2007).…”

Section: Introductionmentioning

confidence: 99%

A New Approach for the Electrochemical Detection of Phenolic Compounds. Part I: Modification of Graphite Surface by Plasma Polymerization Technique and Characterization by Raman Spectroscopy

Günaydın

Şir

Kavlak

et al. 2009

Food Bioprocess Technol

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This paper describes the early stages of preparation of an electrochemical sensor for the detection of phenolic compounds. For this aim, graphite leads were modified by plasma polymerization (PlzP) technique utilizing the monomer N-vinylpyrrolidone (VP) with its conductive property as precursor. The influence of different experimental parameters such as plasma discharge power and exposure time was investigated to optimize the proposed electrode. After the modification process, graphite leads (electrodes) were investigated by differential pulse voltammetry. The voltammograms were recorded between 0 and +1.0 V at 16 mVs −1 . The surface characteristics of the PlzP-VP modified carbon lead surfaces were determined by Raman spectroscopy. It was clearly observed that VP monomer was polymerized to form polyvinylpyrrolidone that is a very well-known adsorbant for phenolics. As expected, it was clearly pointed out that the electrochemical conductivity of the modified carbon leads were varied due to adsorption of a model phenolic compound "gallic acid" at a certain concentration.

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Introduction of sense constructs of cinnamate 4-hydroxylase (CYP73A24) in transgenic tomato plants shows opposite effects on flux into stem lignin and fruit flavonoids

Cited by 39 publications

References 30 publications

Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa

Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa

Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis

A New Approach for the Electrochemical Detection of Phenolic Compounds. Part I: Modification of Graphite Surface by Plasma Polymerization Technique and Characterization by Raman Spectroscopy

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