Introduction of the carrot HSP17.7 into potato (<i>Solanum tuberosum</i> L.) enhances cellular membrane stability and tuberization <i>in vitro</i>

Ahn, Yeh‐Jin; Zimmerman, J. Lynn

doi:10.1111/j.1365-3040.2005.01403.x

Cited by 89 publications

(48 citation statements)

References 48 publications

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“…In line with this concept, recently the carrot sHsp Hsp17.7 was introduced into potato, which exhibited significantly improved cellular membrane stability at high temperatures as evidenced by electrolyte leakage (42). Our findings imply the potential to genetically engineer enhanced stress tolerance in important agronomic crop plants by introducing an Hsp gene encoding Hsps with well defined membrane-interacting features.…”

Section: Discussionmentioning

confidence: 58%

A Mutant Small Heat Shock Protein with Increased Thylakoid Association Provides an Elevated Resistance Against UV-B Damage in Synechocystis 6803

Balogi

Cheregi

Giese

et al. 2008

Journal of Biological Chemistry

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Besides acting as molecular chaperones, the amphitropic small heat shock proteins (sHsps) are suggested to play an additional role in membrane quality control. We investigated sHsp membrane function in the model cyanobacterium Synechocystis sp. PPC 6803 using mutants of the single sHsp from this organism, Hsp17. We examined mutants in the N-terminal arm, L9P and Q16R, for altered interaction with thylakoid and lipid membranes and examined the effects of these mutations on thylakoid functions. These mutants are unusual in that they retain their oligomeric state and chaperone activity in vitro but fail to confer thermotolerance in vivo. We found that both mutant proteins had dramatically altered membrane/lipid interaction properties. Whereas L9P showed strongly reduced binding to thylakoid and model membranes, Q16R was almost exclusively membrane-associated, properties that may be the cause of reduced heat tolerance of cells carrying these mutations. Among the lipid classes tested, Q16R displayed the highest interaction with negatively charged SQDG. In Q16R cells a specific alteration of the thylakoid-embedded Photosystem II (PSII) complex was observed. Namely, the binding of plastoquinone and quinone analogue acceptors to the Q B site was modified. In addition, the presence of Q16R dramatically reduced UV-B damage of PSII activity because of enhanced PSII repair. We suggest these effects occur at least partly because of increased interaction of Q16R with SQDG in the PSII complex. Our findings further support the model that membrane association is a functional property of sHsps and suggest sHsps as a possible biotechnological tool to enhance UV protection of photosynthetic organisms.

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Section: Discussionmentioning

confidence: 58%

A Mutant Small Heat Shock Protein with Increased Thylakoid Association Provides an Elevated Resistance Against UV-B Damage in Synechocystis 6803

Balogi

Cheregi

Giese

et al. 2008

Journal of Biological Chemistry

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“…It is worth adding that expression of some heat shock proteins (eg HSPs 70) can be enhanced by abscisic acid (ABA) (Pareek et al, 1998). Expression of genes inducing HSPs can be an important mechanism of increasing stress tolerance (thermotolerance) through getting better photosynthesis and water and nutrient use efficiency (Camejo et al, 2005) and cellular membrane stability (Ahn and Zimmerman, 2006) or hydration of cellular structures (Wahid and Close, 2007).…”

Section: Expression Of Heat Shock Proteinsmentioning

confidence: 99%

Effect of drought and heat stresses on plant growth and yield: a review

Lipiec¹,

Doussan²,

Nosalewicz³

et al. 2013

International Agrophysics

469

225

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A b s t r a c t. Drought and heat stresses are important threat limitations to plant growth and sustainable agriculture worldwide. Our objective is to provide a review of plant responses and adaptations to drought and elevated temperature including roots, shoots, and final yield and management approaches for alleviating adverse effects of the stresses based mostly on recent literature. The sections of the paper deal with plant responses including root growth, transpiration, photosynthesis, water use efficiency, phenotypic flexibility, accumulation of compounds of low molecular mass (eg proline and gibberellins), and expression of some genes and proteins for increasing the tolerance to the abiotic stresses. Soil and crop management practices to alleviate negative effects of drought and heat stresses are also discussed. Investigations involving determination of plant assimilate partitioning, phenotypic plasticity, and identification of most stress-tolerant plant genotypes are essential for understanding the complexity of the responses and for future plant breeding. The adverse effects of drought and heat stress can be mitigated by soil management practices, crop establishment, and foliar application of growth regulators by maintaining an appropriate level of water in the leaves due to osmotic adjustment and stomatal performance.K e y w o r d s: water stress, high temperature, root and shoot growth, tolerance mechanisms, management practices INTRODUCTIONPlants are frequently exposed to drought and heat stresses that reduce crop yield worldwide. The combined effect of both heat and drought on yield of many crops is stronger than the effects of each stress alone (Dreesen et al., 2012;Rollins et al., 2013).Agricultural water deficit arises from both insufficient rainfall and soil water during the growing season to sustain a high crop yield (Sekhon et al., 2010;Vadez et al., 2011;2012;). Projections show an increase in intense rain events and at the same time reduction in the number of rain days that leads to increased risk of drought (Trenberth, 2011;Vadez et al., 2011). Therefore, under rainfed conditions water scarcity is one of the most widespread limitations to crop production.A period of dry weather, injurious to crops, is often defined as 'drought' that is related to changes in soil and meteorological conditions and not with plant and tissue hydration. Drought stress occurs when the humidity of the soil and the relative air humidity are low and the ambient temperature is high.Predisposition of plants to maintain a high potential of water in the tissues under drought is called dehydration avoidance, and tolerance that determines plant predisposition to survive water deficiency is called drought resistance (Blum, 2005;Vadez et al., 2011). Molecular biologists often report the effect of an exotic gene towards 'drought tolerance' and advertise its expected value in breeding (Blum, 2005).Heat stress or heat wave is defined as the rise in temperature beyond a threshold level for a period sufficient to cause permanent ...

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“…However, only single alleles were tested, and no complementation experiments were performed. Further evidence for the stress-protective role of CI and CII sHSPs in plants has primarily involved constitutive expression or overexpression of a single sHSP in different plant species, including Arabidopsis, rice (Oryza sativa), maize (Zea mays), and carrot (Daucus carota), as well as others (Malik et al, 1999;Ahn and Zimmerman, 2006;Jiang et al, 2009;Sun et al, 2012;Zhou et al, 2012;Mu et al, 2013;Wang et al, 2015). A very restricted set of stress-resistant phenotypes has been reported for sHSP-overexpressing transgenic materials, and these studies have not provided mechanistic insight into sHSP function or distinguished differences between CI and CII sHSPs.…”

mentioning

confidence: 99%

Class I and II small heat-shock proteins protect protein translation factors during heat stress

et al. 2016

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The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones to prevent the irreversible aggregation of heat-sensitive proteins. However, the in vivo activities of sHSPs remain unclear. To investigate the two most abundant classes of plant cytosolic sHSPs (class I [CI] and class II [CII]), RNA interference (RNAi) and overexpression lines were created in Arabidopsis (Arabidopsis thaliana) and shown to have reduced and enhanced tolerance, respectively, to extreme heat stress. Affinity purification of CI and CII sHSPs from heat-stressed seedlings recovered eukaryotic translation elongation factor (eEF) 1B (a-, b-, and g-subunits) and eukaryotic translation initiation factor 4A (three isoforms), although the association with CI sHSPs was stronger and additional proteins involved in translation were recovered with CI sHSPs. eEF1B subunits became partially insoluble during heat stress and, in the CI and CII RNAi lines, showed reduced recovery to the soluble cell fraction after heat stress, which was also dependent on HSP101. Furthermore, after heat stress, CI sHSPs showed increased retention in the insoluble fraction in the CII RNAi line and vice versa. Immunolocalization revealed that both CI and CII sHSPs were present in cytosolic foci, some of which colocalized with HSP101 and with eEF1Bg and eEF1Bb. Thus, CI and CII sHSPs have both unique and overlapping functions and act either directly or indirectly to protect specific translation factors in cytosolic stress granules.

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Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro

Cited by 89 publications

References 48 publications

A Mutant Small Heat Shock Protein with Increased Thylakoid Association Provides an Elevated Resistance Against UV-B Damage in Synechocystis 6803

A Mutant Small Heat Shock Protein with Increased Thylakoid Association Provides an Elevated Resistance Against UV-B Damage in Synechocystis 6803

Effect of drought and heat stresses on plant growth and yield: a review

Class I and II small heat-shock proteins protect protein translation factors during heat stress

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