Introduction of Two Halo-Alkali-thermo-stable Biocatalysts: Purification and Characterization

Kinetics of Halophilic Enzymes

“…This interest is due to their capacity to efficiently catalyze a process and show optimal activities at different salt concentrations. Halophiles are the most probable source of extremozymes, since they are also capable of tolerating alkaline pH and high temperatures, as reported by several authors [68][69][70][71][72][73][74][75][76][77][78][79].…”

“…In a similar manner, Selim et al [74] showed that the protease activity of Natronolimnobius innermongolicus depends on high concentrations of salt to remain active and stable. Proteases purified by Faghihi et al [79] increased their activity in the presence of metallic ions such as Mn 2+ and Cu 2+ , while decreasing activity when exposed to Hg 2+ and Fe 2+ . Both proteases were strongly inhibited by SDS, while DDT, EDTA, and 2-mercaptoethanol may stimulate their activity.…”

“…Halorhabdus, Marinococcus, Micrococcus, Natronococcus, Bacillus, Halobacillus, and Halothermothrix produce extremozymes such as xylanases, amylases, proteases, and lipases. Halophile bacterium Idiomarina produces two extracellular proteases and was isolated in Badab-Sourt, Irán [79]. The same year, Hosseini et al [96] described the isolation of bacteria capable of nitrite reduction that belonged to five different genera: Bacillus, Halobacillus, Idiomarina, Oceanobacillus,…”