Introduction of Various Amine Groups onto Poly(glycidyl methacrylate)-g-MWNTs and their Application as Biosensor Supports

Abstract

A tyrosinase-immobilized biosensor was developed based on various amine-modified multi-walled carbon nanotube (MWNT) supports for the detection of phenolic compounds. MWNTs with various amine groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate (GMA) onto MWNT supports and the subsequent amination of poly(GMA) graft chains. The physical and chemical properties of the poly(GMA)-grafted MWNT supports and the aminated MWNT supports were investigated by SEM, XPS, and TGA. Furthermore, the electrochemical properties of the prepared tyrosinase-modified biosensor based on MWNT supports with amine groups were also investigated. The response of the enzymatic biosensor was in the range of 0.1-0.9 mM for the concentration of phenol in a phosphate buffer solution. Various parameters influencing biosensor performance have been optimized: binder effects, pH, temperature, and the response to various phenolic compounds. The biosensor was tested on phenolic compounds contained in two different commercial red wines.

Radiation‐Induced Graft Polymerization of Vinyl Monomers with Anion Groups onto MWNT Supports and Their Application as Electrogenerated Chemiluminescence (ECL) Biosensors

“…2+ complex onto the TEDA-modified MWNT electrode [10]. The results of this report show that the grafted poly(GMA) chain can act as a polymer binder and the unpaired electron of the immobilized Figure 1: Preparation overview of the ECL-GCE and ECL-SPE based on vinyl polymer-g-MWNT.…”

“…ADPA was synthesized in accordance with a previous paper [10]. In detail, diphosphoric acid (0.562 mol) was dried under reduced pressure for 12 hrs and then dissolved in tetrahydrofuran (THF, 210 mL).…”

A review on surface functionalization of carbon nanotubes: methods and applications

“…With the polymerization of GMA, the epoxy groups in poly-GMA can be modified to alcohol [ 50 ], amine [ 51 ], phosphonic acid, sulfonic acid [ 52 ], and so forth. [ 53 , 54 ]. The epoxy group of poly-GMA can be generated onto a CNT surface by radiation-induced polymerization.…”

“…The physical and chemical properties of the GMA-grafted MWNT and aminated MWNT have been investigated using IR, SEM, XPS, and TGA. However, Chung et al [ 54 ] reported that the increased diameter of the MWNTs indicated the successful attachment of epoxy groups by the radiation-induced graft polymerization of GMA. Amines can be covalently introduced onto MWNT supports with epoxy groups through an SN 2 reaction.…”

Preparation and Characterization of Poly(phenylene sulfide)-Functionalized MWNTs

“…5,6 예를 들어 폴리우레아, 폴리프로필렌, 폴리아크릴산 등 의 고분자를 CNT의 표면에 도입시키면 CNT의 손상이 적고 고분자 매질에 효과적으로 분산시킬 수 있는 것으로 보고되 고 있다. 7,8 한편, 폴리(페닐렌 설파이드)(PPS)는 필름과 섬유뿐만 아니 라 자동차 부품, 기계 부품, 전기•전자 등 다양한 전자제품 및 산업용 분야에 사용되는 고성능 엔지니어링 플라스틱이 다.…”