Amber, fossilized tree resin, found at the Oise River area of the Paris basin (France) was dated as being
55 million years old. Quesnoin, a novel unique pure organic compound, was isolated from Oise amber.
1H and 13C NMR spectroscopic analysis indicated an unknown diterpene skeleton, quesnane. The absolute
configurations of the eight chiral centers of quesnoin were determined to be 4S, 5S, 8R, 9S, 10S, 13S,
14R, and 16S by chiral auxiliary (R)- and (S)-phenylglycine methyl ester derivatization. Quesnoin allowed
us to disclose the tree producer, corresponding to modern Hymenaea oblongifolia, Fabaceae, a subfamily
of Caesalpiniaceae, one of the oldest angiosperm. The presence of the Amazon rainforest tree, H.
oblongifolia, indicated that the climate of the Paris basin might have been tropical in the early Eocene
period, 55 million years ago.